The effect of different dietary zinc sources on mineral deposition and antioxidant indices in rabbit tissues

Klaudia Čobanová, Ľubica Chrastinová, Mária Chrenková, Mária Polačiková, Zuzana Formelová, Oksana Ivanišinová, Miroslav Ryzner, Ľubomíra Grešáková

Abstract

The purpose of this study was to compare the effect of dietary zinc from inorganic and organic sources on the concentration of Zn, Cu, Mn and Fe in plasma, tissues and faeces of rabbits. Simultaneously, the activities of total superoxide dismutase (SOD), specific Cu/Zn SOD, glutathione peroxidase (GPx), lipid peroxidation and total antioxidant capacity (TAC) in liver and kidney were also determined. Ninety-six 49-day-old broiler rabbits were allocated to 4 dietary treatments, each replicated 6 times with 4 animals per replicate. For the subsequent 6 wk, the rabbits were fed an identical basal diet (BD) supplemented with an equivalent dose of Zn (100 mg/kg) from different sources. Group 1 (control) received the unsupplemented BD, while the BD for groups 2, 3 and 4 was supplemented with Zn from Zn sulphate, Zn chelate of glycine hydrate (Zn-Gly) and Zn chelate of protein hydrolysate (Zn-Pro), respectively. The intake of dietary Zn sulphate resulted in an increase in Zn plasma concentration (1.85 vs. 1.48 mg/L; P<0.05) compared to the control group. Feeding the diets enriched with Zn increased the deposition of Zn in the liver (P<0.05), irrespective of the Zn source. The addition of Zn-Pro resulted in significantly higher Cu uptake in liver (P<0.05) than in the control and Zn sulphate group (56.0 vs. 35.0 and 36.7 mg/kg dry matter (DM), respectively). Neither Mn nor Fe concentration in plasma and tissues were affected by dietary Zn supplementation, with the exception of Fe deposition in muscle, which was significantly decreased (P<0.05) in rabbits supplemented with inorganic Zn sulphate compared to control and Zn-Gly group (9.8 vs. 13.3 and 12.2 mg/kg DM, respectively). Intake of organic Zn-Gly significantly increased the activities of total SOD (43.9 vs. 35.9 U/mg protein; P<0.05) and Cu/Zn SOD (31.1 vs. 23.8 U/mg protein; P<0.01) as well as TAC (37.8 vs. 31.2 μmol/g protein; P<0.05) in the kidney when compared to that of the control group. The presented results did not indicate any differences between dietary Zn sources in Zn deposition and measured antioxidant indices in rabbit tissues. Higher dietary Zn intake did not cause any interactions with respect to Mn, Cu and Fe deposition in liver and kidney tissues, but did increase the faecal mineral concentrations. Dietary organic Zn-Gly improved the antioxidant status in rabbit kidney.


Keywords

zinc; rabbit; mineral concentration; antioxidant enzymes; lipid peroxidation

Full Text:

PDF

References

Alscher D.M., Braun N., Biegger D., Stuelten C., Gawronski K., Mürdter T.E., Kuhlmann U., Fritz P. 2005. Induction of metallothionein in proximal tubular cells by zinc and its potential as an endogenous antioxidant. Kidney Blood Press Res., 28: 127-133. https://doi.org/10.1159/000084921

Ao T., Pierce J.L., Power R., Pescatore A.J., Cantor A.H., Dawson K.A., Ford M.J. 2009. Effects of feeding different forms of zinc and copper on the performance and tissue mineral content of chicks. Poultry Sci., 88: 2171-2175. https://doi.org/10.3382/ps.2009-00117

AOAC 2005. Official Methods of Analysis. 18th Edition. Association of Official Analytical Chemists, Gaithersburg, USA.

Bao Y.M., Choct M., Iji P.A., Brueton K. 2007. Effect of organically complexed copper, iron, manganese and zinc on broiler performance, mineral excretion and accumulation in tissues. J. Appl. Poult, Res., 16: 448-455. https://doi.org/10.1093/japr/16.3.448

Benzie I.F.F., Strain J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant Power": The FRAP Assay. Anal. Biochem., 239: 70–76. https://doi.org/10.1006/abio.1996.0292

Bulbul A.T., Bulbul S., Kucukersan M., Sireli M., Eryavuz A. 2008. Effect of dietary supplementation of organic and inorganic Zn, Cu and Mn on oxidant/antioxidant balance in laying hens. Kafkas Univ. Vet. Fak., 14: 19-24.

Bradford M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem., 72: 248-254.

https://doi.org/10.1016/0003-2697(76)90527-3

Casado C., Moya V.J., Pascual J.J., Blas E., Cervera C. 2011. Effect of oxidation state of dietary sunflower oil and dietary zinc and α-tocopheryl acetate supplementation on performance of growing rabbits. World Rabbit Sci., 19: 191-202. https://doi.org/10.4995/wrs.2011.940

Cortese M.M., Suschek C.V., Wetzel W., Kroncke K.D., Kolb-Bachofen V. 2008. Zinc protects endothelial cells from hydrogen peroxide via Nrf2-dependent stimulation of glutathione biosynthesis. Free Radic Biol Med., 44: 2002-2012. https://doi.org/10.1016/j.freeradbiomed.2008.02.013

Farombi E.O., Hansen M., Raven-Haren G., Moller P., Dragsted L.O. 2004. Commonly consumed and naturally occuring dietary substances affect biomarkers of oxidative stress and DNA damage in the healthy rats. Food Chem. Toxicol., 2: 15-22.

Gresakova L., Venglovska K., Cobanova K. 2016. Dietary manganese source does not affect Mn, Zn and Cu tissue deposition and the activity of manganese-containing enzymes in lambs. J. Trace Elem. Med. Biol. 38: 138-143. https://doi.org/10.1016/j.jtemb.2016.05.003

Chrastinová Ľ., Čobanová K., Chrenková M., Poláčiková M., Formelová Z., Lauková A., Ondruška Ľ., Pogány Simonová M., Strompfová V., Mlyneková Z., Kalafová A., Grešáková Ľ. 2016. Effect of dietary zinc supplementation on nutrient digestibility and fermentation characteristics of caecal content in physiological experiment with young rabbits. Slovak J. Anim. Sci., 49: 23-31.

Ivanišinová O., Grešáková Ľ., Ryzner M., Oceľová V., Čobanová K. 2016. Effects of feed supplementation with various zinc sources on mineral concentration and selected antioxidant indices in tissues and plasma of broiler chickens. Acta Vet. Brno, 85: 285-291. https://doi.org/10.2754/avb201685030285

Jo C., Ahn D.U. 1998. Fluorometric analysis of 2-thiobarbituric acid reactive substances in turkey. Poultry Sci., 77: 475-480. https://doi.org/10.1093/ps/77.3.475

King J.C., Brown K.H., Gibson R.S., Krebs N.F., Lowe N.M., Siekmann J.H., Raiten D.J. 2016. Biomarkers of nutrition for development (BOND) – Zinc review. J. Nutr., 146: 858S-885S. https://doi.org/10.3945/jn.115.220079

King J.C., Shames D.M., Woodhouse L.R. 2000. Zinc homeostasis in humans. J. Nutr., 130: 1360S-1366S. https://doi.org/10.1093/jn/130.5.1360S

Kwiecien M., Winiarska-Mieczan A., Milczarek A., Klebaniuk R. 2017. Biological response of broiler chickens to decreasing dietary inclusion levels of zinc glycine chelate. Biol. Trace Elem. Res., 175: 204-213. https://doi.org/10.1007/s12011-016-0743-y

Ma W., Niu H., Feng J., Wang Y., Feng J. 2011. Effects of zinc glycine chelate on oxidative stress, contents of trace elements, and intestinal morphology in broilers. Biol. Trace Elem. Res., 142: 546-556. https://doi.org/10.1007/s12011-010-8824-9

Marklund S., Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47: 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x

Nessrin S., Abdel-Khalek A.M., Gad S.M. 2012. Effect of supplemental zinc, magnesium or iron on performance and some physiological traits of growing rabbits. Asian J. Poult. Sci., 6: 23-30. https://doi.org/10.3923/ajpsaj.2012.23.30

Nutritional Research Council (NRC), 1977. Nutrient requirements of rabbits. National Academies of Science, Washington DC, USA.

Oteiza P.I. 2012. Zinc and the modulation of redox homeostasis. Free Radic. Biol. Med., 53: 1748-1759. https://doi.org/10.1016/j.freeradbiomed.2012.08.568

Paglia D.E., Valentine W.N. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med., 70: 158-169.

Powell S.R. 2000. The antioxidant properties of zinc. J. Nutr., 130: 1447S-1454S. https://doi.org/10.1093/jn/130.5.1447S

Salomonsson A.C., Theander O., Westerlund O. 1984. Chemical characterization of some Swedish cereals whole meal and bran fractions. Swedish J. Agric. Res. 14: 11-117.

Skřivan M., Skřivanová V., Marounek M. 2005. Effects of dietary zinc, iron, and copper in layer feed on distribution of these elements in eggs, liver, excreta, soil, and herbage. Poultry Sci. 84: 1570-1575. https://doi.org/10.1093/ps/84.10.1570

Spears, J.W. 1996. Optimizing mineral levels and sources for farm animals. In Kornegay E.T. (ed). Nutrient Management of Food Animals to Enhance and Protect the Environment, CRC Press, Inc., Boca Raton, FL, 259-275.

Sunder G.S., Kumar V.C., Panda A.K., Raju M.V.L.N., Rao S.V.R. 2013. Effect of supplemental organic Zn and Mn on broiler performance, bone measures, tissue mineral uptake and immune response at 35 d of age. Curr. Res. Poult. Sci., 3: 1-11. https://doi.org/10.3923/crpsaj.2013.1.11

Suttle N.F. 2010. Mineral nutrition of livestock, 4th Edition. CABI Publishing, Wallingford, Oxfordshire, UK. https://doi.org/10.1079/9781845934729.0000

Swiatkiewicz S., Arczewska-Wlosek A., Jozefiak D. 2014. The efficacy of organic minerals in poultry nutrition: review and implications of recent studies. World Poultry Sci. J., 70:475-485. https://doi.org/10.1017/S0043933914000531

Van Soest P.J., Robertson J.B., Lewis B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Diary Sci., 74: 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Wiseman J., Villamide M.J., De Blas C., Carabaño M.J., Carabaño R.M. 1992. Prediction of the digestible energy and digestibility of gross energy of feed for rabbits. 1. Individual classes of feeds. Anim. Feed Sci. Technol., 39: 27-38. https://doi.org/10.1016/0377-8401(92)90029-6

Yan J.Y., Zhang G.W., Zhang C., Tang L., Kuang S.Y. 2017. Effect of dietary organic zinc sources on growth performance, incidence of diarrhoea, serum and tissue zinc concentrations, and intestinal morphology in growing rabbits. World Rabbit Sci., 25: 43-49. https://doi.org/10.4995/wrs.2017.5770

Abstract Views

1412
Metrics Loading ...

Metrics powered by PLOS ALM


 

Cited-By (articles included in Crossref)

This journal is a Crossref Cited-by Linking member. This list shows the references that citing the article automatically, if there are. For more information about the system please visit Crossref site

1. Does Herbal and/or Zinc Dietary Supplementation Improve the Antioxidant and Mineral Status of Lambs with Parasite Infection?
Klaudia Čobanová, Zora Váradyová, Ľubomíra Grešáková, Katarína Kucková, Dominika Mravčáková, Marián Várady
Antioxidants  vol: 9  issue: 12  first page: 1172  year: 2020  
doi: 10.3390/antiox9121172



 

 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs