In vitro caecal fermentation of carbohydrate-rich feedstuffs in rabbits as affected by substrate pre-digestion and donors' diet

C. Ocasio-Vega, R. Abad-Guamán, R. Delgado, R. Carabaño, M.D. Carro, Javier Garcia

Abstract

The influence of substrate pre-digestion and donors’ diet on in vitro caecal fermentation of different substrates in rabbits was investigated. Eight crossbreed rabbits were fed 2 experimental diets containing either low (LSF; 84.0 g/kg dry matter [DM]) or high soluble fibre (HSF; 130 g/kg DM) levels. In vitro incubations were conducted using batch cultures with soft faeces as inoculum and four fibrous or fibre-derived, low-starch and low-protein substrates: D-cellobiose (CEL), sugar beet pectin (PEC), sugar beet pulp (SBP) and wheat straw (WS). Substrates in half of the cultures were subjected to a 2-step pepsin/pancreatin in vitro digestion without filtration, and the whole residue (soluble, insoluble and added enzymes) was incubated at 39°C. Gas production was measured until 144 h, and volatile fatty acid (VFA) production at 24 h incubation was determined. Cultures without substrate (blanks) were included to correct gas production values for gas released from endogenous substrates and added enzymes. Pre-digestion had no influence on in vitro gas production kinetic of WS, and only reduced the time before gas production begins (lag time; by 31%; P=0.042) for SBP, but for both substrates the pre-digestion decreased the molar proportion of acetate (by 9%; P≤0.003) and increased those of propionate and butyrate (P≤0.014). For CEL, the pre-digestion increased the gas and total VFA production (by 30 and 114%), shortened the lag time (by 32%), and only when it was combined with LSF inoculum 38 percentage units of acetate were replaced by butyrate (P≤0.039). Treatments had a minor influence on in vitro fermentation traits of SBP pectin. The results showed that the pre-digestion process influenced the in vitro caecal fermentation in rabbits, but the effects were influenced by donors’ diet and the incubated substrate. Pre-digestion of substrate is recommended before conducting in vitro caecal fermentations. The level of soluble fibre in the donors’ diet also influenced the in vitro caecal fermentation, but its effect depended on the type of substrate.

Keywords

gas production; processing procedure; donors’ diet; rabbits; caecal fermentation; volatile fatty acids

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References

Abad R., Ibáñez M.A., Carabaño R., García J. 2013. Quantification of soluble fibre in feedstuffs for rabbits and evaluation of the interference between the determinations of soluble fibre and intestinal mucin. Anim. Feed Sci. Technol., 182: 61-70. https://doi.org/10.1016/j.anifeedsci.2013.04.001

Abad-Guamán R., Carabaño R., Gómez-Conde M.S., García J. 2015. Effect of type of fiber, site of fermentation, and method of analysis on digestibility of soluble and insoluble fiber in rabbits. J. Anim. Sci., 93: 2860-2871. https://doi.org/10.2527/jas.2014-8767

AOAC. 2000. Official methods of analysis of AOAC International. AOAC: Arlington, VA.

Bauer E., Williams B.A., Voigt C., Mosenthin R., Verstegen M.W.A. 2003. Impact of mammalian enzyme pretreatment on the fermentability of carbohydrate-rich feedstuffs. J. Sci. Food Agric., 83: 207–214. https://doi.org/10.1002/jsfa.1293

Bindelle J., Buldgen A., Boudry C., Leterme P. 2007a. Effect of inoculum and pepsin–pancreatin hydrolysis on fibre fermentation measured by the gas production technique in pigs. Anim. Feed Sci. Technol., 132: 111-122. https://doi.org/10.1016/j.anifeedsci.2006.03.009

Bindelle J., Buldgen A., Lambotte D., Wavreille J., Leterme P. 2007b. Effect of pig faecal donor and of pig diet composition on in vitro fermentation of sugar beet pulp. Anim. Feed Sci. Technol., 132: 212-226. https://doi.org/10.1016/j.anifeedsci.2006.03.010

Bobleter O., Schwald W., Concin R., Binder H. 1986. Hydrolysis of Cellobiose in Dilute Sulpuric Acid and Under Hydrothermal Conditions. J. Carboh. Chem., 5: 387-399. https://doi.org/10.1080/07328308608058843

Bovera F., D'Urso S., Di Meo C., Piccolo G., Calabrò S., Nizza A. 2006. Comparison of rabbit caecal content and rabbit hard faeces as source of inoculum for the in vitro gas production technique. Asian Australas. J. Anim. Sci., 19: 1649. https://doi.org/10.5713/ajas.2006.1649

Bovera F., D’urso S., Di Meo C., Tudisco R., Nizza A. 2009. A model to assess the use of caecal and faecal inocula to study fermentability of nutrients in rabbit. J. Anim. Physiol. Anim. Nutr., 93: 147-156. https://doi.org/10.1111/j.1439-0396.2007.00795.x

Calabrò S., Nizza A., Pinna W., Cutrignelli M.I., Piccolo V. 2010. Estimation of digestibility of compound diets for rabbits using the in vitro gas production technique. World Rabbit Sci., 7: 197-201. https://doi.org/10.4995/wrs.1999.401

Carro M.D., Lebzien P., Rohr K. 1992. Influence of yeast culture on the in vitro fermentation (Rusitec) of diets containing variable portions of concentrates. Anim. Feed Sci. Technol., 37: 209-220. 10.1016/0377-8401(92)90005-Q.

Carro M.D., Ranilla M.J., Tejido M.L. 2005. Using an in vitro gas production technique to examine feed additives: Effects of correcting values for different blanks. Anim. Feed Sci. Technol., 123-124: 173-184. https://doi.org/10.1016/j.anifeedsci.2005.04.045

Coles L.T., Moughan P.J., Darragh A.J. 2005. In vitro digestion and fermentation methods, including gas production techniques, as applied to nutritive evaluation of foods in the hindgut of humans and other simple-stomached animals. Anim. Feed Sci. Technol., 123-124: 421-444. https://doi.org/10.1016/j.anifeedsci.2005.04.021

Falcão-e-Cunha L., Peres H., Freire J.P.B., Castro-Solla L. 2004. Effects of alfalfa, wheat bran or beet pulp, with or without sunflower oil, on caecal fermentation and on digestibility in the rabbit. Anim. Feed Sci. Technol., 117: 131-149. https://doi.org/10.1016/j.anifeedsci.2004.07.014

García J., Gidenne T., Falcão-e-Cunha L., de Blas C. 2002. Identification of the main factors that influence caecal fermentation traits in growing rabbits. Anim. Res., 51: 165-173. https://doi.org/10.1051/animres:2002011

Goering H.K., Van Soest P.J., 1970. Forage fiber analyses (apparatus, reagents, procedures, and some applications). USDA Agr Handb.

Gómez-Conde M.S., García J., Chamorro S., Eiras P., Rebollar P.G., Pérez de Rozas A., Badiola I., de Blas C., Carabaño R. 2007. Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old weaned rabbits. J. Anim. Sci., 85: 3313-3321. https://doi.org/10.2527/jas.2006-777

Gómez-Conde M.S., de Rozas A.P., Badiola I., Pérez-Alba L., de Blas C., Carabaño R., García J. 2009. Effect of neutral detergent soluble fibre on digestion, intestinal microbiota and performance in twenty five day old weaned rabbits. Lives. Sci., 125: 192-198. https://doi.org/10.1016/j.livsci.2009.04.010

Kermauner A., Lavrenčič A. 2013. Relationship between in vitro gas production parameters between predigested and intact commonly used feeds for rabbits. 18th International Symposium on housing and diseases of rabbits, fur providing animals and pet animals. 109-120. Justus-Liebig-Universität Gieβen, Gieβen, Germany.

Lavrenčič A. 2007. The effect of rabbit age on in vitro caecal fermentation of starch, pectin, xylan, cellulose, compound feed and its fibre. Animal, 1: 241-248. https://doi.org/10.1017/S1751731107303467

Marounek M., Skřivanová V., Duškova D. 2000. In vitro caecal fermentation of nitrogenous substrates in rabbits. J. Agric. Sci. 135: 437-442. https://doi.org/10.1017/S0021859699008345

Mateos I., Ranilla J.M., Tejido M.L., Saro C., Kamel C., Carro Travieso, M.D. 2013. The influence of diet on the effectiveness of garlic oil and cinnamaldehyde to manipulate in vitro ruminal fermentation and methane production. Anim. Prod. Sci., 53: 299-307. https://doi.org/10.1071/AN12167

Menke K.H., Raab L., Salewski A., Steingass H., Fritz D., Schneider W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. J. Agric. Sci. 93: 217. https://doi.org/10.1017/S0021859600086305

Mertens D.R. 2002. Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: Collaborative study. J. AOAC Int., 85: 1217-1240.

Morita T., Ozawa M., Ito H., Kimio S., Kiriyama S. 2008. Cellobiose is extensively digested in the small intestine by beta-galactosidase in rats. Nutr. 24: 1199-1204. https://doi.org/10.1016/j.nut.2008.06.029

Nakamura S. 2005. Bioavailability of cellobiose and other non-digestible and/or non-absorbable sugar substitutes and related topics. Nutrition, 21: 1158-1159. https://doi.org/10.1016/j.nut.2005.08.006

Ramos M.A., Carabaño R., Boisen S. 1992. An in vitro method for estimating digestibility in rabbits. J. Appl. Rabbit Res. 15, 938.

Rodríguez-Romero N., Abecia L., Fondevila M. 2011. Effects of levels of insoluble and soluble fibre in diets for growing rabbits on faecal digestibility, nitrogen recycling and in vitro fermentation. World Rabbit Sci., 19: 85-94. https://doi.org/10.4995/wrs.2011.828

Sappok M., Pellikaan W.F., Verstegen M.W.A., Bosch G., Sundrum A., Hendriks W.H. 2013. Large intestinal fermentation capacity of fattening pigs on organic farms as measured in vitro using contrasting substrates. J. Sci. Food and Agric., 93: 2402-2409. https://doi.org/10.1002/jsfa.6075

Schofield P., Pitt R.E., Pell A.N. 1994. Kinetics of fiber digestion from in vitro gas production. J. Anim. Sci., 72: 2980-2991. https://doi.org/10.2527/1994.72112980x

Tran T.H.T., Boudry C., Everaert N., Théwis A., Portetelle D., Daube G., Nezer C., Taminiau B., Bindelle J. 2016. Adding mucins to an in vitro batch fermentation model of the large intestine induces changes in microbial population isolated from porcine feces depending on the substrate. FEMS Microbiol. Ecol., 92. https://doi.org/10.1093/femsec/fiv165

Van der Klis J.D., Jansman A.J. 2002. Optimising nutrient digestion, absorption and gut barrier function in monogastrics: Reality or illusion. Wageningen Academic Publishers, Wagenin, 15-36.

Van Soest P.J. 1994. Nutritional ecology of the ruminant. Cornell University Press.

Vanegas J.L., González J., Carro M.D. 2017. Influence of protein fermentation and carbohydrate source on in vitro methane production. J. Anim. Physiol. Anim. Nutr., 101: e288-e296. https://doi.org/10.1111/jpn.12604

Wallace R.J., Cotta M.A. 1988. Metabolism of nitrogen-containing compounds.: The Rumen Microbial Ecosystem. Elsevier Applied Science, London, 217-250.

Yang H.J., Cao Y.C., Zhang D.F. 2010. Caecal fermentation patterns in vitro of glucose, cellobiose, microcrystalline cellulose and NDF separated from alfalfa hay in the adult rabbit. Anim. Feed Sci. Technol., 162: 149-154. https://doi.org/10.1016/j.anifeedsci.2010.09.008

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1. The effect of cellobiose on the health status of growing rabbits depends on the dietary level of soluble fiber
César Ocasio-Vega, Rebeca Delgado, Rodrigo Abad-Guamán, Rosa Carabaño, Maria Dolores Carro, David Menoyo, Javier García
Journal of Animal Science  vol: 96  issue: 5  first page: 1806  year: 2018  
doi: 10.1093/jas/sky106



 

 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs