In vitro fermentation of different commercially available pectins using inoculum from rabbit caecum

A. Kermauner, A. Lavrenčič

Abstract

In vitro gas production kinetics of eight different commercially available pectin isolates from apple (4), citrus (2) and sugar beet (2), with a degree of methyl esterification (DMet) ranging from 9 to 73%, were determined twice using the mixture of caecum contents of two rabbits of 78 d of age per repetition as inoculum. The cumulated gas production over 60 h of incubation was modelled with the Gompertz model and the estimated and calculated kinetic parameters of each substrate compared. Total potential gas production (B), gas production till 10 h of incubation, maximum fermentation rate (MFR) and time of maximum fermentation rate (TMFR) were affected (P<0.001) by the DMet, but not by the pectin source. Increasing the DMet of pectins increased total potential gas production. The highest B value was determined for Citrus pectin with 72% DMet (Citrus72, 376 mL/g dry matter (DM)), while the lowest for Citrus pectin with only 10% DMet (Citrus10, 289 mL/g DM). Similar trends were also established for the amount of gas produced up to 10 h of incubation, as Citrus72 produced the highest volume of gas (261 mL/g DM) and Citrus10 the lowest (103 mL/g DM). Increasing DMet increased MFR from 9.9 mL/h for Citrus10 to 34.6 mL/h for Citrus72, while TMFR was shortened with the increasing DMet of pectins from 10.3 h for Citrus10 to 6.0 h for both Citrus72 and 60% DMet Apple pectin.

Keywords

in vitro gas production; pectins; degree of methyl esterification; rabbit

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References

Bauer E., Williams B., Bosch M.W., Voigt C., Mosenthin R., Verstegen M.W.A. 2004. Differences in microbial activity of digesta from three sections of the porcine large intestine according to in vitro fermentation of carbohydrate-rich substrates. J. Sci. Food Agric., 84: 2097-2104.

Beuvink J.M.W., Kogut J. 1993. Modelling gas production kinetics of grass silages incubated with buffered ruminal fluid. J. Anim. Sci., 71: 1041-1046.

Bidlack J.E., Buxton D.R. 1992. Content and deposition rates of cellulose, hemicellulose, and lignin during regrowth of forage grasses and legumes. Can. J. Plant Sci., 72: 809-818.

Bindelle J., Buldgen A., Boudry C., Leterme P. 2007. Effect of inoculum and pepsin-pancreatin hydrolysis on fibre fermentation measured by the gas production technique in pigs. Anim. Feed Sci. Technol., 132: 111-122.

Blümmel M., Makkar H.P.S., Becker K. 1997. In vitro gas production: a technique revisited. J. Anim. Physiol. Anim. Nutr., 77: 24-34.

Calabro S., Nizza A., Pinna W., Cutrignelli M.I., Piccolo V. 1999. Estimation of digestibility of compound diets for rabbits using the in vitro gas production technique. World Rabbit Sci., 7: 197-201.

Campbell J.L., Williams C.V., Eisemann J.H. 2002. Fecal inoculum can be used to determine the rate and extent of in vitro fermentation of dietary fiber sources across three lemur species that differ in dietary profile: Varecia variegata, Eulemur fulvus and hapalemur griseus. J. Nutr., 132: 3073-3080.

Carabaño R., García J., de Blas J.C. 2001. Effect of fibre source on illeal apparent digestibility of non-starch polysaccharides in rabbits. Anim. Sci., 72: 343-350.

Dongowski G., Lorenz A. 1998. Unsaturated oligogalacturonic acids are generated by in vitro treatment of pectin with human faecal flora. Carboh. Res., 314: 237-244.

Dongowski G., Lorenz A., Proll J. 2002. The degree of methylation influences the degradation of pectin in the intestinal tract of rats and in vitro. J. Nutr., 132: 1935-1944.

Drochner W., Kerler A., Zacharias B. 2004. Pectin in pig nutrition, a comparative review. J. Anim. Physiol. Anim. Nutr., 88: 367-380.

García J., Carabaño R., de Blas J.C. 1999. Effect of fiber source on cell wall digestibility and rate of passage in rabbits. J. Anim. Sci., 77: 898-905.

García J., Carabaño R., Perez-Alba L., de Blas J.C. 2000. Effect of fiber source on caecal fermentation and nitrogen recycled through caecotrophy in rabbits. J. Anim. Sci., 78: 638-646.

Getachew G., Blümmel M., Makkar H.P.S., Becker K. 1998. In vitro gas measuring techniques for assessment of nutritional quality of feeds: a review. Anim. Feed Sci. Technol., 72: 261-281.

Gidenne T. 1992. Effect of fibre level, particle size and adaptation period on digestibility and rate of passage as measured at the ileum and in the faeces in the adult rabbit. Br. J. Nutr., 67: 133-146. Gidenne T. 2003. Fibres in rabbit feeding for digestive troubles prevention: respective role of low-digested and digestible fibre. Livest. Prod. Sci., 81: 105-117.

Gidenne T., Pinheiro V., Falcao-E-Cunha L. 2000. A comprehensive approach of the rabbit digestion: consequences of a reduction in dietary fibre supply. Livest. Prod. Sci., 64: 225-237.

Gómez-Conde M.S., García J., Chamorro S., Eiras P., Rebollar P.G., Pérez de Rozas A., Badiola I., de Blas C., Carabaño R. 2007. Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old rabbits. J. Anim. Sci., 85: 3313-3321.

Gómez-Conde M.S., Pérez de Rozas A., Badiola I., Pérez-Alba L., de Blas C., Carabaño R., García J. 2009. Effect of neutral detergent soluble fibre on digestion, intestinal microbiota and performance in twenty five day old weaned rabbits. Livest. Sci., 125: 192-198.

Gulfi M., Arrigoni E., Amadò R. 2005. Influence of structure on in vitro fermentability of commercial pectins and partially hydrolysed pectin preparations. Carboh. Polymers, 59: 247-255.

Gulfi M., Arrigoni E., Amadò R. 2006. The chemical characteristics of apple pectin influence its fermentability in vitro. LWT - Food Sci. Technol., 39: 1001-1004.

Jehl N., Gidenne T. 1996. Replacement of starch by digestible fibre in feed for the growing rabbits. 2. Consequences for microbial activity in the caecum and on incidence of digestive disorders. Anim. Feed Sci. Technol., 61: 193-204.

Lavrenčič A. 2007. The effect of rabbit age on in vitro caecal fermentation of starch, pectin, xylan, cellulose, compound feed and its fibre. Animal, 1: 241-248.

Lavrenčič A., Stefanon B., Susmel P. 1997. An evaluation of the Gompertz model in degradability studies of forage chemical components. Anim. Sci., 64: 423-431.

Marounek M., Vovk S.J., Skřivanova V. 1995. Distribution of activity of hydrolytic emzymes in the digestive tract of rabbits. Br. J. Nutr., 73: 463-469.

Marounek M., Vovk S.J., Benda V. 1997. Fermentation patterns in rabbit caecal cultures supplied with plant polysaccharides and lactate. Acta Vet. Brno, 67: 9-13.

Marounek M., Volek Z., Synytsya A., Čopikova J. 2007. Effect of pectin and amidated pectin on cholesterol homeostasis and cecal metabolism in rats fed high-cholesterol diet. Physiol. Res., 56: 433442.

Menke K.H., Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Develop., 28: 375-386.

Novosel’skaya I.L., Voropaeva N.L., Semenova L.N., Rashidova S.Sh. 2000. Trends in the science and application of pectins. Chem. Nat. Compounds, 36: 1-10.

Nyman M., Asp N.-G. 1982. Fermentation of dietary fibre components in the rat intestinal tract. Br. J. Nutr., 47: 357-366.

Olano-Martin E., Gibson G.R., Rastall R.A. 2002. Comparison of the in vitro bifidogenic properties of pectin and pectic polysaccharides. J. Appl. Microbiol., 93: 505-511.

Siragusa R.J., Cerda J.J., Baig M.M., Burgin C.W., Robbins F.L. 1988. Methanol production from the degradation of pectin by human colonic bacteria. Am. J. Clin. Nutr., 47: 848-851.

SaS 1994. SAS/STAT User’s guide (Release 6.03). SAS Institute Inc., Cary NC, USA.

Volek Z., Marounek M., Skřivanova V. 2005. Replacing starch by pectin and inulin in diet of early-weaned rabbits: effect on performance, health and nutrient digestibility. J. Anim. Feed Sci., 14: 327-337.

Willats W.G.T., McCartney L., Mackie W., Knox P. 2001. Pectin: cell biology and prospects for functional analysis. Plant Molec. Biol., 47: 9-27.

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Journal of Animal and Feed Sciences  vol: 25  issue: 3  first page: 266  year: 2016  
doi: 10.22358/jafs/65563/2016



 

 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs