Effects of divergent selection for hind leg muscle volume on its lipid peroxide and glutathione redox status, and fatty acid composition in growing rabbits.

Authors

  • M. Mézes Szent István University
  • K. Balogh Szent István University
  • H. Fébel Research Institute for Animal Breeding and Nutrition
  • Zs. Matics University of Kaposvár
  • M. Fricska Szent István University
  • A. Szabó Kaposvár University
  • Zs. Szendrö Kaposvár University

DOI:

https://doi.org/10.4995/wrs.2009.665

Keywords:

malondialdehyde, glutathione, glutathione peroxidase, fatty acids, rabbit meat

Abstract

Pannon White bucks were selected divergently using CT method by the volume of the hind leg muscle. Animals showed the highest and lowest muscle volumes were selected as minus and plus-selected variants. The male progenies of the minus and plus-selected parents were slaughtered as first generation which was selected again by CT method and the male progenies of the parents were slaughtered. Results in the first and second generation suggest that selection, as a genetic effect did not affect the rate of lipid peroxidation, as was measured by malondialdehyde content and glutathione redox status, as was measured by the reduced glutathione content and glutathione peroxidase activity of the hind leg muscle. However, there were some differences in the fatty acid composition. Significant (P<0.05) difference was found in palmitoleic acid content which was higher in the minus as compared to the plus variants in the second generation, in eicosadienoic acid which was higher in the fi rst as compared to the second generation of minus variants, and total monounsaturated fatty acids which was higher in the minus as compared to the plus variants in the second generation. It means that selection for higher hind leg volume would not causes marked in changes in the rabbit meat quality as measured by lipid peroxide and glutathione status as well as fatty acid composition.

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Author Biographies

M. Mézes, Szent István University

Faculty of Agricultural and Environmental Sciences

K. Balogh, Szent István University

Faculty of Agricultural and Environmental Sciences

Research Group of Animal Breeding and Hygiene, Faculty of Animal Science, University of Kaposvár

Zs. Matics, University of Kaposvár

Faculty of Animal Science, Department of Swine and Small Animal Breeding

M. Fricska, Szent István University

Faculty of Agricultural and Environmental Sciences, Department of Nutrition

A. Szabó, Kaposvár University

Faculty of Animal Science, Department of Swine and Small Animal Breeding

Zs. Szendrö, Kaposvár University

Faculty of Animal Science, Department of Swine and Small Animal Breeding,

References

Balogh K., Matics Zs., Fricska M., Szabó A., Mézes M., Erdélyi M., Szendrő Zs. 2007. Effect of CT based selection for two generations on lipid peroxide and glutathione redox status of some tissues of growing rabbits. In Proc.: 19th Hung. Conf. Rabbit Prod., Kaposvár May 2007. 47-52.

Blasco A., Ouhayoun J. 1996. Harmonization of criteria and terminology in rabbit research. Revised proposal. World Rabbit Sci., 4: 93-99.

Dalle Zotte A. 2002. Perception of rabbit meat quality and major factors influencing rabbit carcass and meat quality. Livest. Prod. Sci., 75: 11-32.

Folch J., Lees M., Stanley G.H.S. 1957. A simple method for the isolation and purification of lipids from animal tissues. J. Biol. Chem. 226: 497-509.

Gray J.I., Gomaa E.A., Buckley D.J., 1996. Oxidative quality and shelf life of meats. Meat Sci., 43: S111-S123.

Gyovai P., Nagy I., Gerencsér Zs., Metzger Sz., Radnai I., Szendrő Zs. 2008. Genetic parameters and trends in thigh muscle volume in Pannon White rabbits. In Proc.: 9th World Rabbit Congress, 10-13 June, 2008. Verona, Italy. 115-120.

Hernandez P., Cesari V., Blasco A. 2008. Effect of genetic rabbit lines on lipid content, lipolytic activities and fatty acid composition of hind leg meat and perirenal fat. Meat Sci., 78: 485-491.

Lawrence R.A., Burk R.F., 1978. Species, tissues and subcellular distribution of non-selenium dependent glutathione peroxidase activity. J. Nutr., 108: 211-215.

Lopez-Bote C.J., Sanz M., Rey A., Castano A., Thos J. 1998. Lower lipid oxidation in the muscle of rabbits fed diets containing oats. Anim. Feed Sci. Technol., 70: 1-9.

Lowry D.H., Rosenbrough N.J., Farr A.L., Randall A.J. 1952. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265-272.

Lukefahr S.D., Odi H.B., Atakora J.K.A. 1996. Mass selection for 70day body weight in rabbits. J. Anim. Sci., 74: 1481-1489.

Maertens L., Huyghebaert G., Delezie E. 2008. Fatty acid composition of rabbit meat when fed a linseed based diet during different periods after weaning. In Proc.: 9th World Rabbit Congress, 10-13 June, 2008. Verona, Italy. 1381-1385.

Mézes M., Eiben Cs., Virág Gy. 1994. Investigations on the glutathione peroxidase activity in blood plasma, red blood cell haemolysate and liver of rabbits. Correlation between enzyme activity and some production traits. In Proc.: 6th Hung. Conf. Rabbit Prod., Kaposvár May 1994. 121-126.

Mézes M., Metzger Sz., Romvári R., Locsmándi L., Petrási Zs., Szabó A., Weber M., Balogh K., Erdélyi M., Szendrő Zs. 2006. Effect of CT based divergent selection on lipid peroxide and glutathione status of some tissues of growing rabbits. In Proc. 18th Hung. Conf. Rabbit Prod., Kaposvár May 2006. 211-214.

Mihara M., Uchiyama M., Fukuzawa K. 1980. Thiobarbituric acid value of fresh homo-genate of rat as a parameter of lipid peroxidation in ageing, CCl4 intoxication and vitamin E deficiency. Biochem. Med., 23: 302-311.

Morrison W.R., Smith L.M. 1964. Preparation of fatty acid methyl esters and dimethyl acetals from lipids with boron trifluoridemethanol. J. Lipid Res,. 5: 600-608.

Nagy I., Ibánez N., Romvári R., Mekkawy W., Metzger Sz., Horn P., Szendrő Zs. 2006. Genetic parameters for growth and in vivo computerised tomography based carcass traits in Pannon White rabbits. Livest. Sci., 104: 46-52.

Ramírez J.A., Díaz I., Pla M., Gil M., Blasco A., Oliver M.A. 2005. Fatty acid composition of leg meat and perirenal fat of rabbits selected by growth rate. Food Chem., 90: 251-256.

Sedlak I., Lindsay R.H., 1968. Estimation of total, protein-bound and non-protein sulfhydryl groups in tissues with Ellmann’s reagent. Anal. Biochem., 25: 192-205.

Statsoft Inc. 1993. Statisticaâ„¢ 4.5 software. User’s Guide, StatSoft Inc., Tulsa.

Szendrő Zs., Metzger Sz., Romvári R., Szabó A., Locsmándi L., Petrási Zs., Nagy I., Nagy Z., Bíró-Németh E., Radnai I., Matics Zs., Horn P. 2008. Effect of divergent selection based on CT measured hind leg muscle volume on productive and carcass traits of rabbits. In Proc.: 9th World Rabbit Congress, 10-13 June, 2008. Verona, Italy. 249-254.

Toyoda H., Himeno S., Imura N. 1989. The regulation of glutathione peroxidase gene expression relevant to species difference and the effects of dietary selenium manipulation. Biochim. Biophys. Acta 1008: 301-308.

Virág Gy., Szendrő Zs., Radnai I., Bíróné Németh E., Romvári R., Mézes M. 1996. Moderate phenotypic relationship between glutathione peroxidase activity and carcass traits in rabbits could be partially determined by genetic effect. In Proc.: 6th World Rabbit Congress, 9-12 July, 1996. Toulouse. France. Vol. 2: 381-383.

Wang S-T., Chen H-W., Sheen L-Y., Lii C-K. 1997. Methionine and cysteine affect glutathione level, glutathione-related enzyme activities and the expression of glutathione S-transferase isozymes in rat hepatocytes. J. Nutr., 127: 2135-2141.

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Published

2010-07-07

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