Polymorphisms of exon 5, exon 7 and intron 10 of MMP2 gene and their association with wool density in Rex rabbits

S.J. Chen, Y.J. Liu, T. Liu, B.J. Chen, Z.L. Gu

Abstract

Wool density is an important index that influences Rex rabbit fur quality. In our earlier studies, we found some important differentially expressed genes in different wool density of Rex rabbit by cDNA microarray. Based on the outcome, we conducted an association study to identify single nucleotide polymorphisms (SNPs) of exon 1, 5, 7 and 10 of matrix metalloproteinase-2 (MMP2) gene and their ligands associated with wool density. The results showed that exon 1 and exon 10 of MMP2 gene did not occur mutation in 100 Rex rabbits, meanwhile 3 SNPs were identified in exon 5, exon 7 and intron 10 of MMP2 gene sequence respectively, the 3 mutation sites were as follows: MMP2-exon 5-26C/G, MMP2-exon 7-101C/T and MMP2-intron 10-6C/T. The 3 SNPs were all in Hardy-Weinberg equilibrium. Phenotypic correlation analysis results showed the 3 mutations lacked significant associations (P>0.05) with the wool density.


Keywords

Rex rabbit; MMP2 gene; SNPs; fur; wool density

Full Text:

PDF

References

Chen S.J., Liu T., Liu Y.J., Dong B., Gu Z.L. 2011a. Gene expression patterns in different wool densities of Rex rabbit using cDNA microarray. Agri. Sci. China, 10: 595-601. https://doi.org/10.1016/S1671-2927(11)60041-2

Chen S.J., Liu T., Liu Y.J., Dong B., Huang Y.T., Gu Z.L. 2011b. Identification of single nucleotide polymorphisms in the CCNA2 gene and its association with wool density in Rex rabbits. Genet. Mol. Res., 4: 3365-3370. https://doi.org/10.4238/2011.November.4.1

Sternlicht M.D., Werb Z. 2001. How matrix metalloproteinases regulate cell behavior. Annu. Rev. Cell. Dev. Biol., 17: 463-516. https://doi.org/10.1146/annurev.cellbio.17.1.463

Nagase H., Visse R., Murphy G. 2006. Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc. Res., 69: 562-573. https://doi.org/10.1016/j.cardiores.2005.12.002

Volcik K.A., Campbell S., Chambless L.E., Coresh J., Folsom A.R., Mosley T.H., Ni H., Wagenknecht L.E., Wasserman B.A., Boerwinkle E. 2010. MMP2 genetic variation is associated with measures of fibrous cap thickness, The Atherosclerosis Risk in Communities Carotid MRI Study. Atheroscler., 1: 188-193. https://doi.org/10.1016/j.atherosclerosis.2009.12.006

Pirilä E., Parikka M., Ramamurthy N.S., Maisi P., McClain S., Kucine A., Tevarhartiala T., Prikk K., Golub L.M., Salo T., Sorsa T. 2002. Chemically modified tetracycline (CMT- 8) and estrogen promote wound healing in ovariectomized rats: effects on matrix metalloproteinase-2, membrane type 1 matrix metalloproteinase, and laminin-5 γ2-chain. Wound Repair Regen., 10: 38-51. https://doi.org/10.1046/j.1524-475X.2002.10605.x

Sotnikova N.Y., Antsiferova Y.S., Posiseeva L.V., Shishkov D.N., Posiseev D.V., Filipova E.S. 2010. Mechanisms regulating invasiveness and growth of endometriosis lesions in rat experimental model and in humans. Fertil. Steril., 93: 2701-2705. https://doi.org/10.1016/j.fertnstert.2009.11.024

Gu Z.L., Ren W.S., Huang R.L., Huang Y.T., Chen B.J. 1999. Study on density of rex rabbit. Chinese J. Rabbit Farming. 4: 18-21. [in Chinese].

Abstract Views

1581
Metrics Loading ...

Metrics powered by PLOS ALM


 

Cited-By (articles included in Crossref)

This journal is a Crossref Cited-by Linking member. This list shows the references that citing the article automatically, if there are. For more information about the system please visit Crossref site

1. Rabbits – their domestication and molecular genetics of hair coat development and quality
K. Dorożyńska, D. Maj
Animal Genetics  vol: 52  issue: 1  first page: 10  year: 2021  
doi: 10.1111/age.13024



 

 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs