Superovulation and expression of follicle-stimulating hormone receptor in young rabbit females

Hao Zhang, Guo Hu Cheng, Yong Jun Li, Miao Yin Cai, Hai Yan Guo, Kang Le Qin


To optimise the use of juvenile in vitro embryo transfer technologies in young rabbit females, superovulation was performed in New Zealand White young rabbit females at different ages and the expression mode of follicle-stimulating hormone receptor (FSHR) was explored using real-time quantitative polymerase chain reaction, and in vitro maturation (IVM) together with fertilisation (IVF) was conducted immediately after superovulation. The results showed that (1) the age factor significantly affected superovulation in young rabbit females, with 60 d as an optimal age; (2) the mRNA level of FSHR exhibited a rising trend, though it was lower at 30 to 40 d of age; (3) the maturation rate of the oocytes from 60 d old rabbits was significantly higher than in those from 50 d old rabbits; (4) the fertilisation rate of oocytes was not significantly different among rabbits 50, 60 and 70 d old.


rabbit; superovulation; follicle-stimulating hormone receptor; in vitro maturation; in vitro fertilisation

Full Text:



Ali A., Benkhalifa M., Miron P. 2006. In-vitro maturation of oocytes: biological aspects. Reprod. Biomed. Online, 13: 437-446.

Brune M., Adams C., Gromoll J. 2010. Primate FSH-receptor promoter nucleotide sequence heterogeneity affects FSHreceptor transcription. Mol. Cell. Endocrinol., 317: 90-98.

Calder M.D., Caveney A.N., Smith L.C., Watson, A. J. 2003. Responsiveness of bovine cumulus-oocyte-complexes (COC) to porcine and recombinant human FSH, and the effect of COC quality on gonadotropin receptor and Cx43 marker gene mRNAs during maturation in vitro. Reprod. Biol. Endocrinol., 1: 14.

Charlton H.M., Parry D., Halpin D.M. G., Webb R. 1982. Distribution of 125I-labelled follicle-stimulating hormone and human chorionic gonadotrophin in the gonads of hypogonadal (hpg) mice. J. Endocrinol., 93: 247-NP.

Dierich A., Sairam M.R., Monaco L., Fimia G.M., Gansmuller A., LeMeur M., Sassone-Corsi P. 1998. Impairing folliclestimulating hormone (FSH) signaling in vivo: targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance. In Proc.: of the National Academy of Sciences, 95: 13612-13617.

Gandolfi F., Brevini T.A.L., Cillo F., Antonini S. 2005. Cellular and molecular mechanisms regulating oocyte quality and the relevance for farm animal reproductive efficiency. Rev. Sci. Tech. OIE., 24: 413.

Gou K.M., Guan H., Bai J.H., Cui X.H., Wu Z.F., Yan F.X., An X.R. 2009. Field evaluation of juvenile in vitro embryo transfer (JIVET) in sheep. Anim. Reprod. Sci., 112: 316-324.

Izquierdo D., Villamediana P., López-Bejar M., Paramio M. T. 2002. Effect of in vitro and in vivo culture on embryo development from prepubertal goat IVM–IVF oocytes. Theriogenology, 57: 1431-1441.

Kalita A., Baishya G., Bhattacharya M. 2000. Development of ovary in Assam goat from birth to six months of age-A histomorphometrical study. Indian J. Animal Sci., 70: 248-250.

Kalita A., Baishya G., Chakravarty P. 2001. Age-related morphological characterization of follicles and oocytes in Assam goat from birth to 6 months of age. Indian J. Animal Sci., 71: 534-536.

Kelly J.M., Kleemann D.O., Walker S.K. 2005. Enhanced efficiency in the production of offspring from 4-to 8-weekold lambs. Theriogenology, 63: 1876-1890.

Koeman J., Keefer C.L., Baldassarre H., Downey B.R. 2003. Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media following laparoscopic recovery. Theriogenology, 60: 879-889.

Leoni G.G., Succu S., Satta V., Paolo M., Bogliolo L., Bebbere D., Spezzigu A., Madeddu M., Berlinguer F., Ledda S., Naitana S. 2009. In vitro production and cryotolerance of prepubertal and adult goat blastocysts obtained from oocytes collected by laparoscopic oocyte-pick-up (LOPU) after FSH treatment. Reprod. Fert. Develop., 21: 901-908.

Moor R.M., Trounson A.O. 1977. Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent developmental capacity. J. Reprod. Fertil., 49: 101-109.

Oxberry B.A., Greenwald G.S. 1982. An autoradiographic study of the binding of 125I-labeled follicle-stimulating hormone, human chorionic gonadotropin and prolactin to the hamster ovary throughout the estrous cycle. Biol. Reprod., 27: 505-516.

Paramio M.T. 2010. In vivo and in vitro embryo production in goats. Small Ruminant Res., 89: 144-148.

Shima K., Kitayama S., Nakano R. 1987. Gonadotropin binding sites in human ovarian follicles and corpora lutea during the menstrual cycle. Obstet. Gynecol., 69: 800-806.

Sugimoto H., Kida Y., Miyamoto Y., Kitada K., Matsumoto K., Saeki K., Taniguchi, T., Hosoi Y. 2012. Growth and development of rabbit oocytes in vitro: Effect of fetal bovine serum concentration on culture medium. Theriogenology, 78: 1040-1047.

Tassell R., Chamley W.A., Kennedy J.P. 1978. Gonadotrophin levels and ovarian development in the neonatal ewe lamb. Austral J. Biol. Sci., 31: 267-274.

Tsuji K., Sowa M., Nakano R. 1985. Relationship between human oocyte maturation and different follicular sizes. Biol. Reprod., 32: 413-417.

Wilson T., Wu X.Y., Juengel J.L., Ross I.K., Lumsden J.M., Lord E.A., Dodds K.G., Walling G.A., McEwan J.C., O’Connell A.R., McNatty K.P., Montgomery G.W. 2001. Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK- 6) that is expressed in both oocytes and granulosa cells. Biol. Reprod., 64: 1225-1235.

Yeh S.P., Fan Y.K., Tseng J.K. 2002. The developmental competence and factors influencing the in vitro production of cattle embryos using oocytes derived from juvenile calves. J. Agr. Assoc. China, 32: 93-105.

Zaid A., Hughes H.G., Porceddu E., Nicholas F.W. 1999. Glossary of biotechnology and genetic engineering. FAO.

Zhang S.B., Dattatreyamurty B., Reichert Jr L.E. 1991. Differential Roles of High and Low Affinity Guanosine 5'-Triphosphate Binding Sites in the Regulation of Follicle-Stimulating Hormone Binding to Receptor and Signal Transduction in Bovine Calf Testis Membranes*. Endocrinology, 128: 295-302.

Abstract Views

Metrics Loading ...

Metrics powered by PLOS ALM


Cited-By (articles included in Crossref)

This journal is a Crossref Cited-by Linking member. This list shows the references that citing the article automatically, if there are. For more information about the system please visit Crossref site

1. Oxidative Effects of Potassium Dichromate on Biochemical, Hematological Characteristics, and Hormonal Levels in Rabbit Doe (Oryctolagus cuniculus)
Chongsi Mary Momo, Ngoula Ferdinand, Ngouateu Omer Bebe, Makona Alexane Marquise, Kenfack Augustave, Vemo Bertin Narcisse, Tchoffo Herve, Tchoumboue Joseph
Veterinary Sciences  vol: 6  issue: 1  first page: 30  year: 2019  
doi: 10.3390/vetsci6010030


 Universitat Politècnica de València


Official journal of the World Rabbit Science Association (WRSA)


e-ISSN: 1989-8886     ISSN: 1257-5011