Detection of specific antibodies against strains of enteropathogenic escherichia coli (EPEC) in the rabbit by an elisa using 94-kilodalton proteins.

D.G.F. Vandekerchove, J. E. Peeters

Abstract

A protein band of 94 kilodalton specífic for EPEC strains, was detected and partially purified by elution electrophoresis. The protein band was used in an ELISA at 1 µg per well. Sera were taken every fortnight after experimental infections with six enteropathogenic Escherichia coli (EPEC) strains belonging to the pathotypes 1+/0109, 2+/0128, 2+/0132, 3-/015, 4+/026 and 8+/0103, and with three non pathogenic E. coli strains. Furthermore, sera were collected from slaughter rabbits of commercial rabbitries with known EPEC status. The two groups of sera were tested at a dilution of 1 :625 with the ELISA based on the 94-kilodalton proteins. The serological results were compared with the EPEC status of fecal samples of the corresponding animals. The test offers a specificity of 73 % and a sensitivity of 96 % (P<0,05).


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 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs