Effect of divergent selection for uterine capacity on embryonic survival and development at 30 h post-mating in unilaterally ovariectomized rabbit females

R. Peiro, M. Gallego, A. Blasco, M.A. Santacreu

Abstract

Uterine capacity has been proposed as an indirect way to increase litter size. The aim of this work is to study the effect of a divergent selection for uterine capacity (UC) on reproductive traits at 30 h post mating in unilaterally ovariectomized (ULO) females. A total of 62 ULO females from the high line (selected to increase UC) and 39 ULO females from the low line (selected to decrease UC) were used. Ovulation rate was estimated as the number of corpora haemorrhagica and early embryonic survival was estimated as the ratio between number of embryos and ovulation rate. No differences in ovulation rate and early embryonic survival at 30 h post mating were found between high and low lines. Selection for UC did not change the embryonic stage of development either, the majority of embryos being at 4-cell stage. Additionally, the embryos were evaluated according to morphological criteria and more than 95% of the embryos were evaluated as good or fair quality. No differences in embryonic morphological criteria between high and low lines were found either. Thus, selection for UC did not modify the early embryonic survival and development in ULO females at 30 h post mating.


Keywords

rabbit; divergent selection; early embryonic survival; embryo development; uterine capacity

Full Text:

PDF

References

Argente M.J., Santacreu M.A., Climent A., Bolet G., Blasco A. 1997. Divergent selection for uterine capacity in rabbits. J. Anim. Sci., 75: 2350-2354.

Blasco A., Argente M.J., Haley C., Santacreu M.A. 1994. Relationships between components of litter size in unilaterally ovariectomized and intact rabbit does. J. Anim. Sci., 72: 3066-3072.

Blasco A., Bidanel J.P., Bolet G., Haley C.S., Santacreu M.A. 1993. The genetics of prenatal survival of pigs and rabbits: a review. Livest. Prod. Sci., 37: 1-21. doi:10.1016/0301-6226(93)90061-L

Blasco A., Ortega J.A., Climent A., Santacreu M.A. 2005. Divergent selection for uterine capacity in rabbits. I. Genetic parameters and response to selection. J. Anim. Sci., 83: 2297-2302.

BOE. 2005. Available at: http://www.ub.edu/rceue/archivos/RD_1201_2005.pdf. Accessed February 2015.

Bolet G., Theau-Clément M. 1994. Fertilization rate and preimplantation embryonic development in two rabbit strains of different fecundity, in purebreeding and crossbreeding. Anim. Reprod. Sci., 36: 153-162. doi:10.1016/0378-4320(94)90062-0

Bolet G., Santacreu M.A., Argente M.J., Climent A., Blasco A. 1994. Divergent selection for uterine efficiency in unilaterally ovariectomized rabbits. I. Phenotypic and genetic parameters. In. Proc.: 5th World Congress on Genetics Applied to Livestock Production, 7-12 August 1994, Guelph, Canada. 19: 261-264.

Christenson R.K., Leymaster K.A., Young L.D. 1987. Justification of unilateral hysterectomy–ovariectomy as a model to evaluate uterine capacity in swine. J. Anim. Sci., 67: 738-744.

García-Ximénez F., Vicente J.S. 1992. Effect of ovarian cystic or haemorrhagic follicles on embryo recovery and survival after transfer in hCG ovulated rabbits. Reprod. Nutr. Dev., 32: 143-149. doi:10.1051/rnd:19920207

Hunter R.H.F. 1982. Fisiología y tecnología de la reproducción de la hembra de los animales domésticos, first ed. Acribia, Zaragoza.

Ménézo Y., Renard J.P. 1991. La vie de l'oeuf avant l'implantation, In: Thibault, M.C., (Ed.), La reproduction chez les mammifères et l'home. INRA, Toulouse, pp. 339-358.

Mocé M.L., Santacreu M.A., Climent A., Blasco A. 2004. The effect of divergent selection for uterine capacity on prenatal survival in rabbits: maternal and embryonic genetic effects. J. Anim. Sci., 82: 68-73.

Mocé M.L., Santacreu M.A., Climent A., Blasco, A. 2005. Divergent selection for uterine capacity in rabbits. III. Responses in uterine capacity and its components estimated with a cryopreserved control population. J. Anim. Sci., 83: 2308-2312.

Peiró R., Santacreu M.A., Climent A., Blasco A. 2007. Early embryonic survival and embryo development in two divergent lines of rabbits selected for uterine capacity. J. Anim. Sci., 85: 1634-1639. doi:10.2527/jas.2006-737

Santacreu M.A., Mocé M.L., Climent A., Blasco A. 2005. Divergent selection for uterine capacity in rabbits. II. Correlated response in litter size and its components estimated with a cryopreserved control population. J. Anim. Sci., 83: 2303-2307.

SAS. 2008. Statistical analysis system user’s guide, Version 9.2. SAS Institute, Inc., Cary.

Shulman A., Bennun I., Ghetler Y., Kaneti H., Shilon M., Beyth Y. 1993. Relationship between embryo morphology and implantation rate after in-vitro fertilization treatment in conception cycles. Fertil. Steril., 60: 123-126.

Torres S., Hulot F., Meunier, M., Sevellec C. 1987. Comparative study of preimplantation development and embryonic loss in two rabbit strains. Reprod. Nutr. Dev., 27: 707-714. doi:10.1051/rnd:19870510

Veeck L.L., Maloney M. 1986. Insemination and fertilization, in: Jones H.W., Jones G.S., Hodgen G.D., Rosenwaks Z. (Eds.), In-vitro fertilization-norfolk. Williams and Wilkins, Baltimore, pp. 168-200.

Abstract Views

2759
Metrics Loading ...

Metrics powered by PLOS ALM




 Universitat Politècnica de València

Official journal of the World Rabbit Science Association (WRSA)

e-ISSN: 1989-8886     ISSN: 1257-5011  https://doi.org/10.4995/wrs