Different serum complements affect the in vitro development and hatching rates of rabbit zygotes. A technical note.

Abstract:

The effect of two different types of serum supplements Ham's F-10 medium on the development of rabbit zygotes to the lastocyst stage was tested. The in vitro development was improved when ovulated-doe serum, instead young-rabbit serum was added to lam's F-10 medium. The development to the 16-cell, compacted norula-early blastocyst, blastocyst or hatched stage at 24, 48 and 168 hours of culture were 44%, 54%, 100% and 92% vs 0%, 19%, 75% and 56%, respectively. Moreover, the established conditions of culture (39⁰ C, 7% co₂ in air and 15 mM sodium bicarbonate) associated with the ovulated-doe serum in Ham's F-10 could enhance the in vítro development of rabbit zygotes up to the hatching stage (90-100%).

Show more Show less