Different serum complements affect the in vitro development and hatching rates of rabbit zygotes. A technical note.

 M.J. Escribá, F. García Ximénez

Abstract

The effect of two different types of serum supplements Ham's F-10 medium on the development of rabbit zygotes to the lastocyst stage was tested. The in vitro development was improved when ovulated-doe serum, instead young-rabbit serum was added to lam's F-10 medium. The development to the 16-cell, compacted norula-early blastocyst, blastocyst or hatched stage at 24, 48 and 168 hours of culture were 44%, 54%, 100% and 92% vs 0%, 19%, 75% and 56%, respectively. Moreover, the established conditions of culture (390 C, 7% co2 in air and 15 mM sodium bicarbonate) associated with the ovulated-doe serum in Ham's F-10 could enhance the in vítro development of rabbit zygotes up to the hatching stage (90-100%).


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 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs