Fasting effects on in vitro fermentation pattern of rabbit caecal contents

 F. Piattoni, D. Demeyer, L. Maertens

Abstract

Caecal contents were collected from six 16h fasted (F) and six non fasted (NF) rabbits, sacrificed between 9 and 1 0am. They were incubated for 24h/39ºC and net productions of volatile fatty acids (VFA) and methane were determined. In a separate preliminary experiment, caecal contents obtained from a F and a NF rabbit were incubated with starch, cellulose or pectins. In F animals, total VFA concentration was lower (P<0.001) and caecal pH significantly higher (P=0.015) than in NF enes: 6.5 vs 6.2. Molar proportions of propionate were higher than butyrate (11.3 vs 4.0%) in F rabbits. However, the latter became higher after 24h of in vitro incubation. Significant (P<0.001) less total VFA (844 vs 1776 µmol/flask) but higher (P=0.003) amounts of methane (271 vs 131 mmoles/mole VFA) were formed using caecal contents from F rabbits. Calculated hydrogen recoveries were indicative of reductive acetogenesis (RA) in NF but not in F animals (49 vs 73%). When one of the substrates was added, effect of fasting was not any more clear for VFA but still for methane and hydrogen recoveries.


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1. Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits
Rodrigo Abad-Guzmán, Jose Antonio Larrea-Dávalos, Rosa Carabaño, Javier García, Maria Dolores Carro
World Rabbit Science  vol: 26  issue: 3  first page: 227  year: 2018  
doi: 10.4995/wrs.2018.9726



 

 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs