European ring-test on in vivo determination of digestibility in rabbits: reproducibility of a reference method in comparison with domestic laboratory procedures

 J.M. Pérez, C. Cervera, L. Falcao e Cunha, L. Maertens, M.J. Villamide, G. Xiccato

Abstract

A collaborative study involving six European laboratorios from five countries (Belgium, France, Italy, Portugal and Spain) was undertaken to assess the reproducibility of an European standard method for in vivo determination of diet digestibility and to compare the results obtained with domestic procedures. The European method was carried out on 49-day-old rabbits caged individually and fed ad libitum; it included an adaptation period of 7 days followed by a collection period of 4 days. The digestibility coefficients of dry matter (dDM), organic matter (dOM), energy (dE), crude protein (dCP), crude fibre (dCF), and the digestible energy (DE) content of two diets were measured in each laboratory by both standard and domestic methods. On the whole, 260 digestive balances were carried out on the basis of 8 to 10 rabbits mínimum per diet per each method. In comparison with the laboratory procedures the standard method improved the reproducibility notably for the digestibility of dry matter (among-laboratory standard deviation: SL= 0.70 vs 0.92), crude fibre (SL=2.49 vs 5.02) and DE content (SL= 0.18 vs 0.26 MJ/kg DM).


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References

AOAC, 1988. Guidelines for collaborative study procedure to validate characteristics of a method of analysis. J. Assoc. Off Anal. Chem., 71, 161-171.

AOAC, 1990. Official methods of analysis (15th Ed.). Association of Official Analytical Chemists Arlington, VA.

BELTRAN M.T., MARTINEZ J., FERNANDEZ J., CERVERA C., 1984. Estimacion del valor nutritivo de diversas materias primas para conejos. Proc. 3rd World Rabbit Congress, vol.1, 265-272.

BOURDILLON A., CARRÉ B., CONAN L., DUPERRAY J., HUYGHEBAERT G., LECLERCQ B., LESSIRE M., MCNAB J., WISEMAN J., 1990. European reference method for the in vivo determination of metabolisable energy with adult cockerels. Br. Poult. Sci., 31, 557-565. https://doi.org/10.1080/00071669008417287

CHEEKE P.R., 1987. Rabbit feeding and nutrition. Academic Press !ne., Orlando, USA, 376p.

COLIN M., LEBAS F., 1976. Méthodes d'études de la digestibilité des aliments chez le lapin. l. Périodicité des collectes. Sci. Tech. Anim. Lab., 1, 129-133.

DIXON W. J., 1953. Processing data for outliers. Biometrics, 9, 7 4-89. https://doi.org/10.2307/3001634

FEKETE S., GIPPERT T., 1986. Digestibility and nutritive value of nineteen important feedstuffs for rabbits. J. Appl. Rabbit Res., 9, 103-108.

HORWITZ W, KAMPS L.R., BOYER K.W., 1980. Quality assurance in the analysis of foods for trace constituents. J. Assoc. Off Anal. Chem., 63, 1344-1354. https://doi.org/10.1093/jaoac/63.6.1344

INRA, 1989. L'alimentation des animaux monogastriques : porc, lapin, volailles. INRA Editions, Paris, 282 p.

MAERTENS L., JANSSEN W.M.M., STEENLAND E., WOLTERS D.F., BRANJE H.E.B., JAGER F., 1990. Tables de composition, de digestibilité et de valeur énergétique des matieres premieres pour lapins. 5emes Journ. Rech. Cunicole Fr., Paris, 12-13 Décembre 1990. Communication nº 57, 57.1-57.9. ITAVI Ed., Paris.

PÉREZ J.M., 1991. Intéret et limites des modeles de prévision de la valeur énergétique des aliments destinés au porc. These de Doctorat, Université de Montpellier 11, 196 p.

PEREZ J.M., LEBAS F., 1992. Peut-on estimer la valeur énergétique des aliments destinés aux lapins ? Cuniculture, 19, 27 1-274.

PEREZ J.M., BOURDILLON A., JARRIN D., LAMBOLEY B., LEBAS F., LE NAOUR J., WIDIEZ J.L., 1994. Application de la méthode européenne standardisée de mesure in vivo de la valeur énergétique des aliments destinés au lapin comparaisons inter-laboratoires. 6emes Journ. Rech. Cunicole Fr., La Rochelle, 6-7 Décembre 1994,pp. 365-374.ITAVI Ed.,Paris.

PEREZ J.M., LEBAS F., GIDENNE T., MAERTENS L., XlCCATO G., PARIGI-BINI R., DALLE ZOTTE A., Cossu M.E., CARAZZOLO A., VILLAMIDE M.J., CARABAÑO R., FRAGA M.J., RAMOS M.A., CERVERA C., BLAS E., FERNANDEZ J., FALCAO e CUNHA L., BENGALA FREIRE J., 1995. European reference method for in vivo determination of diet digestibility in rabbits. World Rabbit Sci., 3, 41-43. https://doi.org/10.4995/wrs.1995.239

SAS, 1988. SAS/STAT User's Guide (Release 6.03). SAS lnstitute Inc., Cary, NC, USA, 1028 p.

SAUVANT D., BRETTE C., 1983. Les tolérances sur les caractéristiques portées sur les étiquettes. In L' évolution de la législation en alimentation animale, Cycle Approfondi dAlimentation Animale, INA-PG, 24 mars 1983, H1-H16. ADEPRINA Ed., Paris.

VAN SOEST P.J., ROBERTSON J.B., LEWIS B., 1991. Methods for dietary fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74, 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

VILLAMIDE M.J., RAMOS M.A., 1994. Length of collection period and number of rabbits in digestibility assays. World Rabbit Sci., 2, 29-35. https://doi.org/10.4995/wrs.1994.215

VILLAMIDE M.J., FRAGA M.J., DE BLAS J.C., 1991. Effect of type of basal diet and rate of inclusion on the evaluation of protein concentrates with rabbits. Anim. Prod., 52, 215-224. https://doi.org/10.1017/S0003356100005869

XICCATO G., Cossu M.E., CARAZZOLO A., CARABAÑO R., RAMOS M., 1994. Evaluation in vitro de la valeur nutritive des aliments pour lapins. Efficacité de différents enzymes digestifs. 6emes Journ. Rech. Cunicole Fr., La Rochelle, 6-7 Décembre 1994, pp. 375-383. ITAVI Ed., Paris.

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1. EUROPEAN REFERENCE METHOD FOR IN VIVO DETERMINATION OF DIET DIGESTIBILITY IN RABBITS
  Pérez J.M, Lebas F., Gidenne T., Maertens L., Xiccato G., Parigi-Bini R., Dalle Zotte A.
World Rabbit Science  vol: 3  issue: 1  year: 2010  
doi: 10.4995/wrs.1995.239



 

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