Utilization of rabbit skin as edible film for environmentally friendly food packaging using glycerol as plasticizer

Jajang Gumilar

https://orcid.org/0000-0003-1234-9851

Indonesia

Padjadjaran University image/svg+xml

Department of Animal Product Technology, Faculty of Animal Husbandry, Universitas Padjadjaran

Wendry S. Putranto

https://orcid.org/0000-0002-3865-7361

Indonesia

Padjadjaran University image/svg+xml

Department of Animal Product Technology, Faculty of Animal Husbandry, Universitas Padjadjaran

Andry Pratama

https://orcid.org/0000-0001-8950-6718

Indonesia

Padjadjaran University image/svg+xml

Department of Animal Product Technology, Faculty of Animal Husbandry, Universitas Padjadjaran

Rani Maharani

https://orcid.org/0000-0001-8156-9773

Indonesia

Padjadjaran University image/svg+xml

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran

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Accepted: 2025-03-11

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Published: 2025-06-30

DOI: https://doi.org/10.4995/wrs.2025.23032
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Keywords:

rabbit skin, edible film, food packaging, glycerol, plasticizer

Supporting agencies:

Universitas Padjadjaran

Ministry of Research Technology and Higher Education

Abstract:

The growing rabbit population has directly contributed to increased meat yields and higher production of by-products, including skins. The skin of local Indonesian rabbits is mostly used as fish feed and fertilizer due to the relatively poor quality of their fur. Rabbit skin can potentially be used as a more economical product such as edible film, due to its high protein content of 67.3% and collagen content of 28.8%. Protein and collagen from rabbit skin can be produced into good quality gelatine. Gelatine plays a vital role in developing biodegradable edible film packaging materials. Gelatine films offer a strong barrier against light, air and odour transfer, but they can be too brittle as packaging materials. This problem needs to be overcome by adding plasticizers such as glycerol. The purpose of this study was to investigate the effect of glycerol on the functional groups and physical, mechanical and colour properties of edible films made from rabbit skin gelatine. This experiment employed a completely randomised design with five glycerol treatments (10, 20, 30, 40, and 50%). Each treatment was replicated four times. Data were analysed using ANOVA followed by Tukey’s test. The results show that there is a good affinity between biopolymers from rabbit skin gelatine and glycerol as indicated by the formation of Fourier transform infrared (FTIR) bands. The level of glycerol used has a significant effect (P<0.05) on the mechanical, physical and colour properties. The best edible film from rabbit skin gelatine is produced with 10% glycerol.

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References:

Abedinia A., Ariffin F., Huda N., Nafchi A.M. 2018. Preparation and characterization of a novel biocomposite based on duck feet gelatin as alternative to bovine gelatin. Int. J. Biol. Macromol., 109: 855-862. https://doi.org/10.1016/j.ijbiomac.2017.11.051

Ahmad A.A., Sarbon N.M. 2021. A comparative study: Physical, mechanical and antibacterial properties of bio-composite gelatin films as influenced by chitosan and zinc oxide nanoparticles incorporation. Food Biosci., 43: 101250. https://doi.org/10.1016/j.fbio.2021.101250

Ahmad T., Ismail A., Ahmad S.A., Khalil K.A., Leo T.K., Awad E.A., Imlan J., Sazili A.Q. 2018. Effects of ultrasound assisted extraction in conjugation with aid of actinidin on the molecular and physicochemical properties of bovine hide gelatin. Molecules, 23: 730. https://doi.org/10.3390/molecules23040730

Alavi S., Thomas S., Sandeep K.P., Kalarikkal N., Varghese J., Yaragalla S. (Eds.). 2015. Polymers for packaging application. Apple Academic Press: Mistwell Crescent Oakville, Canada.

Al-Hassan A.A. 2024. Development and characterization of camel gelatin films: Influence of camel bone age and glycerol or sorbitol on film properties. Heliyon, 10: e30338. https://doi.org/10.1016/j.heliyon.2024.e30338

Al-Hassan A.A., Norziah M.H. 2012. Starch–gelatin edible films: Water vapor permeability and mechanical properties as affected by plasticizers. Food Hydrocoll., 26: 108-117. https://doi.org/10.1016/j.foodhyd.2011.04.015

Aitboulahsen M., Chairi H., Laglaoui A., Zantar S., Abdelhay A., Bakkali M., Zerrouk M.H. 2020. Gelatin/pectin-based film incorporated with essential oils: Functional characteristics and shelf life extension of tilapia fillets under refrigeration. J. Food Safety, 40: e12774. https://doi.org/10.1111/jfs.12774

Altiok D., Altiok E., Tihminlioglu F. 2010. Physical, antibacterial and antioxidant properties of chitosan films incorporated with thyme oil for potential wound healing applications. J. Mater. Sci. Mater. Med., 21: 2227-2236. https://doi.org/10.1007/s10856-010-4065-x

AOAC. 2012. Official methods of analysis of AOAC International, 19th ed. Association of Official Analytical Chemists, Gaithersburg, MD, USA.

Arfat Y.A., Benjakul S., Prodpran T., Sumpavapol P., Songtipya P. 2016. Physicomechanical characterization and antimicrobial properties of fish protein isolate/fish skin gelatin-zinc oxide (ZnO) nanocomposite films. Food Bioprocess Technol., 9: 101-112. https://doi.org/10.1007/s11947-015-1602-0

Arvanitoyannis I., Nakayama A., Aiba S. 1998. Edible films made from hydroxypropyl starch and gelatin and plasticized by polyols and water. Carbohydr. Polym., 36: 105-119. https://doi.org/10.1016/S0144-8617(98)00017-4

Ballesteros-Martínez L., Pérez-Cervera C., Andrade-Pizarro R. 2020. Effect of glycerol and sorbitol concentrations on mechanical, optical, and barrier properties of sweet potato starch film. NFS J., 20: 1-9. https://doi.org/10.1016/j.nfs.2020.06.002

Ben Z.Y., Samsudin H., Yhaya M.F. 2022. Glycerol: its properties, polymer synthesis, and applications in starch based films. Eur. Polym. J., 175: 111377. https://doi.org/10.1016/j.eurpolymj.2022.111377

Bergo P., Sobral P.J.A. 2007. Effects of plasticizer on physical properties of pigskin gelatin films. Food Hydrocoll., 21: 1285-1289. https://doi.org/10.1016/j.foodhyd.2006.09.014

Bergo P., Moraes C.F., Sobral P.J.A. 2013. Effects of plasticizer concentration and type on moisture content in gelatin films. Food Hydrocoll., 32: 412-415. https://doi.org/10.1016/j.foodhyd.2013.01.015

Castro J.I., Navia-Porras D.P., Arbeláez Cortés J.A., Mina Hernández J.H., Grande-Tovar C.D. 2022. Synthesis, characterization, and optimization studies of starch/chicken gelatin composites for food-packaging applications. Molecules, 27: 2264. https://www.mdpi.com/1420-3049/27/7/2264

Cazón P., Vázquez M., Velázquez G. 2020. Regenerated cellulose films with chitosan and polyvinyl alcohol: Effect of the moisture content on the barrier, mechanical and optical properties. Carbohydr. Polym., 236: 116031. https://doi.org/10.1016/j.carbpol.2020.116031

Cheng M., Deng J., Yang F., Gong Y., Zhao N., Zhang X. 2003. Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions. Biomaterials 24: 2871-2880. https://doi.org/10.1016/S0142-9612(03)00117-0

Christoph R., Schmidt B., Steinberner U., Dilla W., Karinen R. 2012. Glycerol. Ullmann’s Encycl. Ind. Chem., 17: 67-82. https://doi.org/10.1002/14356007.a12

Cuq B., Gontard N., Cuq J.L., Guilbert S. 1997. Selected functional properties of fish myofibrillar protein-based films as affected by hydrophi hilic plasticizers. J. Agric. Food Chem., 45: 622-626. https://doi.org/10.1021/jf960352i

da Nóbrega-Santos E., de Albuquerque Sousa T.C., de Santana Neto D.C., Grisi C.V.B., da Silva Ferreira V.C., da Silva F.A.P. 2022. Edible active film based on gelatin and Malpighia emarginata waste extract to inhibit lipid and protein oxidation in beef patties. LWT - Food Sci. Technol., 154: 112837. https://doi.org/10.1016/j.lwt.2021.112837

Fasano E., Bono-Blay F., Cirillo T., Montuori P., Lacorte S. 2012. Migration of phthalates, alkylphenols, bisphenol, A and di(2-ethylhexyl) adipate from food packaging. Food Control, 27:132-138.

Ge Y., Li Y., Bai Y., Yuan C., Wu C., Hu Y. 2020. Intelligent gelatin/oxidized chitin nanocrystals nanocomposite films containing black rice bran anthocyanins for fish freshness monitorings. Int. J. Biol. Macromol., 155: 1296-1306. https://doi.org/10.1016/j.ijbiomac.2019.11.101

Getachew A.T., Ahmad R., Park J.S., Chun B.S. 2021. Fish skin gelatin based packaging films functionalized by subcritical water extract from spent coffee ground. Food Packag. Shelf Life, 29: 100735. https://doi.org/10.1016/j.fpsl.2021.100735

GMIA (Gelatin Manufacturers Institute of America). 2012. Gelatin handbook. North America.

Gómez-Guillén M.C., Giménez B., López-Caballero M.E., Montero M.P. 2011. Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocoll., 25: 1813-1827. https://doi.org/10.1016/j.foodhyd.2011.02.007

Gontard N., Duchez C., Cuq J.L., Guilbert S. 1994. Edible composite films of wheat gluten and lipids: Water

vapor permeability and other physical properties. Int. J. Food Sci. Technol., 29: 39-50. https://doi.org/10.1111/j.1365-2621.1994.tb02045.x

Gumilar J., Pratama A. 2018. Production and characterization of halal gelatin made from chicken intestine. J. Agroind. Technol., 28: 75-81. https://doi.org/10.24961/j.tek.ind.pert.2018.28.1.75

Gumilar J., Pratama A., Wulandari E., Tavares L., Maharani R. 2024a. Effect of glycerol on physical, mechanical and color properties of chicken intestine gelatin films. Asian J. Dairy Food Res., 1-8. https://doi.org/10.18805/ajdfr.DRF-444

Gumilar J., Mahardika D., Suryaningsih L. 2024b. The effect of chloride Acid submersion with various concentrations on viscosity, pH, and gel strength of rabbit skins gelatin. Int. J. Multidiscip. Educ. Res., 9: 16-19.

Hassan B., Chatha S.A.S., Hussain A.I., Zia K.M., Akhtar N. 2018. Recent advances on polysaccharides, lipids and protein based edible films and coatings: A review. Int. J. Biol. Macromol., 109: 1095-1107. https://doi.org/10.1016/J.IJBIOMAC.2017.11.097

Hajji S., Kchaou H., Bkhairia I., Salem R.B.S.B., Boufi S., Debeaufort F., Nasri M. 2021. Conception of active food packaging films based on crab chitosan and gelatin enriched with crustacean protein hydrolysates with improved functional and biological properties. Food Hydrocoll., 116: 106639. https://doi.org/10.1016/j.foodhyd.2021.106639

Herzog M.H., Francis G., Clarke A. 2019. Understanding statistics and experimental design. Springer Nature Switzerland, AG. Gewerbestrasse 11, 6330 Cham, Switzerland.

Hosseini S.F., Rezaei M., Zandi M., Farahmandghavi F. 2015. Fabrication of bionanocomposite films based on fish gelatin reinforced with chitosan nanoparticles. Food Hydrocoll., 44: 172-182. https://doi.org/10.1016/j.foodhyd.2014.09.004

Jusoha N.A.M., Isab M.I.N., Sarbon N.M. 2022. Physical, mechanical and antioxidant properties of chicken skin gelatin films incorporated with virgin coconut oil. Biocatal. Agric. Biotechnol., 45: 102525. https://doi.org/10.1016/j.bcab.2022.102525

Liu C., Huang J., Zheng X., Liu S., Lu K., Tang K., Liu J. 2020. Heat sealable soluble soybean polysaccharide/gelatin blend edible films for food packaging applications. Food Packag. Shelf Life, 24: 100485. https://doi.org/ 10.1016/j.fpsl.2020.100485

Loo C.P.Y., Norizah M., Sarbon. 2020. Chicken skin gelatin films with tapioca starch. Food Biosci., 35: 100589. https://doi.org/10.1016/j.fbio.2020.100589

Lupina K., Kowalczyk D., Lis M., Raszkowska-Kaczor A., Drozłowska E. 2022. Controlled release of water-soluble astaxanthin from carboxymethyl cellulose/gelatin and octenyl succinic anhydride starch/gelatin blend films. Food Hydrocoll., 123: 107179. https://doi.org/10.1016/j.foodhyd.2021.107179

Mariniello L., Di Pierro P., Esposito C., Sorrentino A., Masi P., Porta R. 2003. Preparation and mechanical properties of edible pectin–soy flour films obtained in the absence or presence of transglutaminase. J. Biotechnol., 102: 191-198.

Neves E.M.P.X., Pereira R.R., da Silva Pereira G.V., da Silva Pereira G.V., Vieira L.L., Lourenço L.D.F.H. 2019. Effect of polymer mixture on bioplastic development from fish waste. Boletim do Instituto de Pesca, 45. https://doi.org/10.20950/1678-2305.2019.45.4.518

Nilsuwan K., Benjakul S., Prodpran T., de la Caba K. 2019a. Fish gelatin monolayer and bilayer films incorporated with epigallocatechin gallate: Properties and their use as pouches for storage of chicken skin oil. Food Hydrocoll., 89: 783- 791. https://doi.org/10.1016/j.foodhyd.2018.11.056

Nilsuwan K., Guerrero P., de la Caba K., Benjakul S., Prodpran T. 2019b. Properties of fish gelatin films containing epigallocatechin gallate fabricated by thermocompression molding. Food Hydrocoll., 97: 105236. https://doi.org/10.1016/j.foodhyd.2019.105236

Nor M.H.M., Nazmi N.N.M., Sarbon N.M. 2017. Effects of plasticizer concentrations on functional properties of chicken skin gelatin films. Int. Food Res. J., 24: 1910-1918. https://doi.org/10.1016/j.fbio.2018.06.006

Nordin N., Othman S.H., Rashid S.A., Basha R.K. 2020. Effects of glycerol and thymol on physical, mechanical, and thermal properties of corn starch films. Food Hydrocoll., 106: 105884. https://doi.org/10.1016/j.foodhyd.2020.105884

Nur Hazirah M.A.S.P., Isa M.I.N., Sarbon N.M. 2016. Effect of xanthan gum on the physical and mechanical properties of gelatin-carboxymethyl cellulose film blends. Food Packag. Shelf Life, 9: 55-63. https://doi.org/10.1016/j.fpsl.2016.05.008

Ockerman W., Hansen C.L. 2000. Animal by-product processing & utilization. 1st Ed. CRC Press. Oluba

O.M., Obi C.F., Akpor O.B., Ojeaburu S.I., Ogunrotimi F.D., Adediran A.A., Oki M. 2021. Fabrication and characterization of keratin starch biocomposite film from chicken feather waste and ginger starch. Sci. Rep., 11: 8768. https://doi.org/10.1038/s41598-021-88002-3

Paudel S., Regmi S., Janaswamy S. 2023. Effect of glycerol and sorbitol on cellulose-based biodegradable films. Food Packag., Shelf Life, 37: 101090. https://doi.org/10.1016/j.fpsl.2023.101090

Plackett D. 2011. Biopolymers—new materials for sustainable films and coatings; John Wiley & Sons, Ltd.: Hoboken, NJ,USA.

Pudel F., Benecke P., Vosmann K., Matthäus B. 2016. 3-MCPDand glycidyl esters can be mitigated in vegetable oils by use of short path distillation. Eur. J. Lipid. Sci. Technol., 118: 396-405. https://doi.org/10.1002/ejlt. 201500095

Raharjo Y.C. 2008 Rabbit production development under bird flu situation in Indonesia. In Proc.: MEKARN Rabbit Conference: Organic rabbit production from forages (Editors: Reg Preston and Nnguyen Van Thu). Cantho University. Vietnam. 25-27 November 2008. http://www.mekarn.org/prorab/raha.htm

Ramos M., Valdes A., Beltran A., Garrigos M.C. 2016. Gelatinbased films and coatings for food packaging applications. Coatings, 6: 41. https://doi.org/10.3390/coatings6040041

Rather J.A., Akhter N., Ashraf Q.S., Mir S.A., Makroo H.A., Majid D., Barba F.J., Khaneghah A.M., Dar N. 2022. A comprehensive review on gelatin: understanding impact of the sources, extraction methods, and modifications on potential packaging applications. Food Packag. Shelf Life, 34: 100945. https://doi.org/10.1016/j.fpsl.2022.100945

Salimiraad S., Safaeian S., Basti A.A., Khanjari A., Nadoushan R.M. 2022. Characterization of novel probiotic nanocomposite films based on nano chitosan/nano cellulose/gelatin for the preservation of fresh chicken fillets. LWT, 162: 113429. https://doi.org/10.1016/j.lwt.2022.113429

Sanches M.A.R., Camelo-Silva C., Tussolini L., Tussolini M., Zambiazi R.C., Pertuzatti P.B. 2021. Development, characterization and optimization of biopolymers films based on starch and flour from jabuticaba (Myrciaria cauliflora) peel. Food Chem., 343: 128430

Sogut E., Balqis A.I., Hanani Z.N., Seydim A.C. 2019. The properties of κ-carrageenan and whey protein isolate blended films containing pomegranate seed oil. Polymer Test., 77: 105886. https://doi.org/10.1016/j.polymertesting.2019.05.002

Soo P.Y., Sarbon N.M. 2018. Preparation and characterization of edible chicken skin gelatin film incorporated with rice flour. Food Packag. Shelf Life, 15: 1-8. https://doi.org/10.1016/j.fpsl.2017.12.009

Sothornvit R., Krochta J.M. 2000. Plasticizer effect on oxygen permeability of beta-lactoglobulin films. J. Agric. Food Chem., 48: 6298-6302. https://doi.org/10.1021/jf000836l

Suderman N., Sarbon N.M. 2019. Preparation and characterization of gelatin-based films with the incorporation of Centella asiatica (L.) urban extract. Food Res., 3: 306-314. https://doi.org/10.26656/fr.2017.3(5).045

Suderman N., Isab M.I.N., Sarbona N.M.. 2018. The effect of plasticizers on the functional properties of biodegradable gelatin-based film: A review. Food Biosci., 24: 111-119. https://doi.org/10.1016/j.fbio.2018.06.006

Tao Y.R. 1994. Studies on the quality of rex rabbit fur. World Rabbit Sci., 2: 21-24. https://doi.org/10.4995/wrs.1994.213

Thomazine M., Carvalho R.A., Sobral P.J.A. 2006. Physical properties of gelatin films plasticized by blends. of glycerol and sorbitol. J. Food Sci., 70: E172-E176. https://doi.org/10.1111/j.1365-2621.2005.tb07132.x

Tongdeesoontorn W., Rawdkuen S. 2019. Gelatin-based films and coatings for food packaging applications. Research Unit of Innovative. Food Packaging and Biomaterials. https://doi.org/10.1016/B978-0-08-100596-5.22598-5

Tongdeesoontorn W., Mauer L.J., Wongruong S., Sriburi P., Rachtanapun P. 2012. Mechanical and physical properties of cassava starch-gelatin composite films. Int. J. Polym. Mater., 61: 778-792. https://doi.org/10.1080/00914037.2011.610049

Toniasso D.P.W., da Silva C.G., Junior B.d.B., Somacal S., Emanuelli T., Kubota E.H., Dornelles R.C.P., Mello R. 2022. Collagen extracted from rabbit: Meat and byproducts: isolation and physicochemical assessment. Food Res. Int., 162: 111967. https://doi.org/10.1016/j.foodres.2022.111967

Uranga J., Leceta I., Etxabide A., Guerrero P., De La Caba K. 2016. Cross-linking of fish gelatins to develop sustainable films with enhanced properties. Eur. Polym. J., 78: 82-90. https://doi.org/10.1016/j.eurpolymj.2016.03.017

Wittaya T. 2013. Influence of type and concentration of plasticizers on the properties of edible film from mung bean proteins. Kmitl Sci. Technol. J.,13: 51-58.

Wulandari D., Hermiyati I., Iswahyuni I., Tawarniate A.Z. 2022. Production and characterisation of gelatin from rabbit bone as bioplastics material by acid pre-treatment. World Rabbit Sci., 30: 83-93. https://doi.org/10.4995/wrs.2022.16639

Yeddes W., Djebali K., Wannes W A., Horchani-Naifer K., Hammami M., Younes I., Tounsi M.S. 2020. Gelatinchitosan-pectin films incorporated with rosemary essential oil: optimized formulation using mixture design and response surface methodology. Int. J. Biol. Macromol., 154: 92-103. https://doi.org/10.1016/j.ijbiomac.2020.03.092

Zhang H., Liang Y., Li X., Kang H. 2020. Effect of chitosan-gelatin coating containing nano-encapsulated tarragon essential oil on the preservation of pork slices. Meat Sci., 166: 108137. https://doi.org/10.1016/j.meatsci.2020.108137

Zhou C., Li Y., Yu X., Yang H., Ma H., Yagoub A.E.A., Otu P.N.Y. 2016. Extraction and characterization of chicken feet soluble collagen. LWT-Food Sci. Technol., 74: 145-153. https://doi.org/10.1016/j.lwt.2016.07.024

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