Plasma albumin measurements in New Zealand white rabbits

G.O. Evans

Abstract

Following reports in the literature of problems with serum albumin measurements using bromocresol green dye binding methods, the method was re-examined using fifty rabbit plasma samples and solutions of different rabbit fractionated proteins. The value found in this study (albumin: 33 ± 2 g/1) was generally lower than in most of the other studies (27 to 43 g/1) and the ranges narrower. None of the samples analysed gave values for albumin which were higher than those for total protein (53 ± 3 g/1).


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References

BORTOLOTTI A., CASTELLI D., BONATI M, 1989. Hematology and serum chemistry values of adult, pregnant and newborn New Zealand rabbits (Oryctolagus cuniculus). Lab. Anim. Sci., 39, 437-439.

COWIE J.R., EVANS G.0., 1983. Plasma albumin determination on the Cobas Bio centrifugal analyzer using bromocresol green. J. Automat. Chem., 5, 153-154. https://doi.org/10.1155/S1463924683000371

EVANS G.0., PARSONS C.E., 1988. A comparison of two dye binding methods for the determination of dog, rat and human plasma albumins. J. Comp. Path., 98, 453-460. https://doi.org/10.1016/0021-9975(88)90093-X

EVANS G.0., PARSONS C.E., 1990. Plasma albumin determination in the Cobas Mira : effect of measurement wavelength. Clin. Chem., 36, 176-177. https://doi.org/10.1093/clinchem/36.1.176

FAVARATO M., ZATTA P., 1990. Chemico-clinical characteristics of rabbit serum. J. Appl. Rabbit Res;, 13, 14-15.

Fox R.R, 1989. The rabbit. In: The clinical chemistry of laboratory animals. Eds Loeb W.F. and Quimby F. W., Pergamon Press, Inc. New York, 45-46.

GUSTAFSSON J.E.C., 1976. lmproved specificity of serum albumin determination and estimation of "acute phase reactants" by use of the bromocresol green reaction. Clin. Chem., 22, 616-622. https://doi.org/10.1093/clinchem/22.5.616

HALL R.E., 1992. Clinical pathology of laboratory animals. In : Animal models in toxicology. Eds Gad S.C. and Chengelis C.P., Marcel Dekker Inc. New York, 765-811.

JENSEN A.L., WED0 E, BANTZ M., 1992. Critical differences of clinical chemistry components in blood from laboratory rabbits. Scand. J. Lab. Anim., 19, 179-184.

KANEK0 J.I., 1989. Clinical Biochemistry of Domestic Animals. 4th ed., Academic Press Inc., California, USA, 865.

K0ZMA C, MACKLIN W., CUMMINS L.M., MAUER R., 1974. Anatomy, physiology and biochemistry of the rabbit. In: The biology of the Laboratory Rabbit. Eds Weisbroth S.H., Flatt R.E. and Kraus A.L. Academic Press, New York, 60-69. https://doi.org/10.1016/B978-0-12-742150-6.50008-6

WEBSTER D., 1977. The immediate reaction between bromocresol green and serum as a measure of albumin content. Clin. Chem., 23, 663-665. https://doi.org/10.1093/clinchem/23.4.663

WILLS J.E., R0WLANDS M.A., N0RTH D.C., EVANS G.O., 1993. Effect of serial cardiac puncture and blood collection procedures in the rabbit. Anim. Technol., 44, 39-52.

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 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs