Preslaughter feed withdrawal and sex influences on rabbit physiological response and meat quality
Submitted: 2024-01-12
|Accepted: 2024-08-02
|Published: 2024-09-30
Copyright (c) 2024 Anne-Sophie Larivière-Lajoie, Joanie Lemieux, Dany Cinq-Mars, Frédéric Guay, Antoni Dalmau, Linda Saucier
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
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Keywords:
fasting, gastrointestinal tract measurements, meat quality, microbiology, rabbits
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Abstract:
Although preslaughter feed withdrawal is commonly used in the livestock industry to reduce the risk of viscera puncture during evisceration, the optimal feed withdrawal time (FWT) appears to be species dependent and is not yet well defined in rabbits. This study aimed to evaluate the impact of three preslaughter FWTs while considering the effect of sex on physiological response and meat quality of rabbits. Three FWTs (4, 11 and 18 h) were tested using 144 recently weaned Grimaud rabbits fed until they reached an average commercial slaughter live body weight of 2.5±0.2 kg. Rabbits were allocated into 24 cages, with six animals in each cage. Eight cages (four cages for females and four cages for males) were assigned to each of the three FWTs. Blood lactate concentrations measured at exsanguination did not raise concerns related to stress. Female rabbits subjected to 4 h FWT had a higher gastrointestinal tract weight compared to male rabbits and to other females subjected to 11 and 18 h FWT (P=0.04). Stomach content weights were lower after 18 h FWT compared to 4 and 11 h (P=0.002) for both sexes, while caecum weights were not affected by FWT. Caecum pH was higher after 11 and 18 h FWT compared to 4 h (P=0.02). Technological meat quality was not particularly affected by FWT, except for Longissimus lumborum drip loss which was lower for rabbits after 18 h FWT compared to 4 h FWT (P=0.005). With respect to microbiological meat quality, the end of shelf life was reached after 10 days for vacuum-packed hind legs from rabbits after 11 h FWT, as Escherichia coli cell counts for four out of the eight hind legs analysed were higher than 3 Log10 colony-forming units (CFU)/10 g. For the other two FWT groups, the end of shelf life was reached after 15 d, at which point the cell counts for all hind legs were ≥3 Log10 CFU/10 g for E. coli and ≥7 Log10 CFU/g for total aerobic mesophilic and presumptive lactic acid bacteria (the maximum threshold concentrations according to current regulations). Our results demonstrate that when transport (30 min) and lairage (30 min) times are short (1 h in total), an 18-h preslaughter feed withdrawal for both male and female rabbits can allow the gut to empty properly with limited effect on meat quality.
References:
Adzitey F., Nurul H. 2011. Pale soft exudative (PSE) and dark firm dry (DFD) meats: causes and measures to reduce these incidences-a mini review. Int. Food Res. J., 18: 11-20. https://doi.org/10.3923/ijps.2017.81.87
Bertol T.M., Ellis M., Ritter M.J., McKeith F.K. 2005. Effect of feed withdrawal and handling intensity on longissimus muscle glycolytic potential and blood measurements in slaughter weight pigs. J. Anim. Sci., 83: 1536-1542. https://doi.org/10.2527/2005.8371536x
Bianchi M., Petracci M., Venturi L., Cremonini M.A., Cavani C. 2008. Influence of pre-slaughter fasting on live weight loss, carcass yield and meat quality in rabbits. In Proc.: 9th World Rabbit Congress, 10-13 June 2008, Verona, Italy, 1313-1318.
Carmichael E.B., Strickland J.T., Driver R.L. 1945. The contents of the stomach, small intestine, cecum and colon of normal and fasting rabbits. Am. J. Physiol., 143: 562-566. https://doi.org/10.1152/ajplegacy.1945.143.4.562
Cavani C., Bianchi M., Lazzaroni C., Luzi F., Minelli G., Petracci M. 2000. Influence of type of rearing, slaughter age and sex on fattening rabbit: II. meat quality. In Proc.: 7th World Rabbit Congress, 4-7 June 2000, Valencia, Spain, 567-572.
Cavani C., Petracci M., Trocino A., Xiccato G. 2009. Advances in research on poultry and rabbit meat quality. Ital. J. Anim. Sci., 8: 741-750. https://doi.org/10.4081/ijas.2009.s2.741
CCAC. 2009. The care and use of farm animals in research, teaching and testing. Canadian Council on Animal Care. http://www.ccac.ca/Documents/Standards/Guidelines/Farm_Animals.pdf. Accessed February 2023.
CIE. 1976. Colorimetry. International Commission on Illumination. Publication 15 Vienna, Austria: Bureau Central de la CIE. http://www.cie.co.at/. Accessed December 2023.
Cliche R., Sylvestre A. 2009. Gestion et organisation de l’élevage. In: Centre de Référence en Agriculture et Agroalimentaire du Québec (ed.). L’élevage commercial du lapin. Québec, Canada, 215-250.
Coppings R.J., Ekhator N., Ghodrati A. 1989. Effects of antemortem treatment and transport on slaughter characteristics of fryer rabbits. J. Anim. Sci., 67: 872-880. https://doi.org/10.2527/jas1989.674849x
Cornejo-Espinoza J.G., Rodríguez-Ortega L.T., Pro-Martínez A., González-Cerón F., Conde-Martínez V.F., Ramírez-Guzmán M.E., López-Pérez E., Hernández-Cázares A.S. 2016. Efecto del ayuno ante mortem en el rendimiento de la canal y calidad de la carne de conejo. Arch. Zootec., 65: 171-175. https://doi.org/10.21071/az.v65i250.484
Dalla Costa F.A., Devillers N., Paranhos da Costa M.J.R., Faucitano L. 2016. Effects of applying preslaughter feed withdrawal at the abattoir on behaviour, blood parameters and meat quality in pigs. Meat Sci., 119: 89-94. https://doi.org/10.1016/j.meatsci.2016.03.033
Dalle Zotte A. 2000. Main factors influencing the rabbit carcass and meat quality. In Proc.: 7th World rabbit congress, 4-7 July, 2000. Valencia, Spain. 507-537.
Dalle Zotte A. 2002. Perception of rabbit meat quality and major factors influencing the rabbit carcass and meat quality. Livest. Prod. Sci., 75: 11-32. https://doi.org/10.1016/S0301-6226(01)00308-6
Dalle Zotte A., Cullere M., Rémignon H., Alberghini L., Paci G. 2016. Meat physical quality and muscle fibre properties of rabbit meat as affected by the sire breed, season, parity order and gender in an organic production system. World Rabbit Sci., 24: 145-154. https://doi.org/10.4995/wrs.2016.4300
DGSAIA. 2011. Manuel des méthodes d’inspection des abattoirs. Direction générale de la santé animale et de l’inspection des aliments. http://www.mapaq.gouv.qc.ca/fr/Publications/Manueldesmethodes_inspectionabattoirs.pdf.
Driessen B., Freson L., Buyse J. 2020. Fasting finisher pigs before slaughter influences pork safety, pork quality and animal welfare. Animals, 10: 1‑10. https://doi.org/10.3390/ani10122206
Eicher S.D., Rostagno M.H., Lay D.C. 2017. Feed withdrawal and transportation effects on Salmonella enterica levels in market-weight pigs. J. Anim. Sci., 95: 2848-2858. https://doi.org/10.2527/jas.2017.1454
Eikelenboom G., Bolink A.H., Sybesma W. 1991. Effects of feed withdrawal before delivery on pork quality and carcass yield. Meat Sci., 29: 25-30. https://doi.org/10.1016/0309-1740(91)90020-Q
Faucitano L., Saucier L., Correa J.A., Méthot S., Giguère A., Foury A., Mormède P., Bergeron R. 2006. Effect of feed texture, meal frequency and pre-slaughter fasting on carcass and meat quality, and urinary cortisol in pigs. Meat Sci., 74: 697-703. https://doi.org/10.1016/j.meatsci.2006.05.023
Faucitano L., Chevillon, P, Ellis M. 2010a. Effects of feed withdrawal prior to slaughter and nutrition on stomach weight, and carcass and meat quality in pigs. Livest. Sci., 127: 110-114. https://doi.org/10.1016/j.livsci.2009.10.002
Faucitano L., Ielo M.C., Ster C., Lo Fiego D.P., Methot S., Saucier L. 2010b. Shelf life of pork from five different quality classes. Meat Sci., 84: 466-469. https://doi.org/10.1016/j.meatsci.2009.09.017
Fazio F., Casella S., Giudice E., Giannetto C., Piccione G. 2015. Evaluation of secondary stress biomarkers during road transport in rabbit. Livest. Sci., 173: 106-110. https://doi.org/10.1016/j.livsci.2015.01.006
FDA. 1999. Microbiological safety evaluations and recommendations on sprouted seed. Food and Drug Administration. https://www.fda.gov/Food/GuidanceRegulation/ucm078789.htm
Frobose H.L., Dritz S.S., Tokach M.D., Prusa K.J., DeRouchey J.M., Goodband R.D., Nelssen J.L. 2014. Effects of preslaughter feed withdrawal time on finishing pig carcass, body weight gain, and food safety characteristics in a commercial environment. J. Anim. Sci., 92: 3693-3700. https://doi.org/10.2527/jas.2013-7367
Gill C.O. 2000. HACCP in primary processing: Red meat. In: Brown M. (ed.). HACCP in the meat industry. CRC Press, Boca Raton, Florida, USA, 81-122.
Guidenne T., Lebas F. 2005. Le comportement alimentaire du lapin. In Proc.: 11e Journées de la Recherche Cunicole, 29-30 November 2005, Paris, France, 183-196.
Honikel K.O. 1998. Reference methods for the assessment of physical characteristics of meat. Meat Sci., 49: 447-457.
Hörnicke H. 1981. Utilization of caecal digesta by caecotrophy (soft faeces ingestion) in the rabbit. Livest. Prod. Sci., 8: 361‑366. https://doi.org/10.1016/0301-6226(81)90054-3
Huff-Lonergan E., Baas T.J., Malek M., Dekkers J.C.M., Prusa K., Rothschild M.F. 2005. Correlations among selected pork quality traits. J. Anim. Sci., 80: 617-627. https://doi.org/10.2527/2002.803617x
Hulot F., Ouhayoun J. 1999. Muscular pH and related traits in rabbits: A review. World Rabbit Sci., 7: 15-36. https://doi.org/10.4995/wrs.1999.378
Jolley P.D. 1990. Rabbit transport and its effects on meat quality. Appl. Anim. Behav. Sci., 28: 119-134. https://doi.org/10.1016/0168-1591(90)90049-J
Joseph K.J., Awosanya B., Adebua B.A. 1994. The effects of preslaughter withholding of feed and water from rabbits on their carcass yield and meat quality. Niger. J. Anim. Prod., 21: 164‑169. https://doi.org/10.51791/njap.v21i1.1186
Kassambara A., Mundt F. 2020. Factoextra: Extract and Visualize the Results of Multivariate Data Analyses. R Package Version 1.0.7. https://CRAN.R-project.org/package=factoextra. Accessed February 2023
Koné A.P., Cinq-Mars D., Desjardins Y., Guay F., Gosselin A., Saucier L. 2016. Effects of plant extracts and essential oils as feed supplements on quality and microbial traits of rabbit meat. World Rabbit Sci., 24: 107-119. https://doi.org/10.4995/wrs.2016.3665
Koné A.P., Zea J.M.V., Gagné D., Cinq-Mars D., Guay F., Saucier L. 2018. Application of Carnobacterium maltaromaticum as a feed additive for weaned rabbits to improve meat microbial quality and safety. Meat Sci., 135: 174‑188. https://doi.org/10.1016/j.meatsci.2017.09.017
Koné A.P., Desjardins Y., Gosselin A., Cinq-Mars D., Guay F., Saucier L. 2019. Plant extracts and essential oil product as feed additives to control rabbit meat microbial quality. Meat Sci., 150: 111‑121. https://doi.org/10.1016/j.meatsci.2018.12.013
Lambertini L., Vignola G., Badiani A., Zaghini G., Formigoni A. 2006. The effect of journey time and stocking density during transport on carcass and meat quality in rabbits. Meat Sci., 72: 641-646. https://doi.org/10.1016/j.meatsci.2005.09.012
Langlois I., Planché A., Boysen S.R., Abeysekara S., Zello G.A. 2014. Blood concentrations of, D- and, L-lactate in healthy rabbits. J. Small Anim. Pract., 55: 451-456. https://doi.org/10.1111/jsap.12247
Larivière-Lajoie A.-S., Cinq-Mars D., Guay F., Binggeli S., Dalmau A., Saucier L. 2021. Hierarchical clustering as a tool to develop a classification scheme for rabbit meat quality. World Rabbit Sci., 29: 129-149. https://doi.org/10.4995/wrs.2021.14368
Larivière-Lajoie A.-S., Laforge P., Vincent A.T., Binggeli S., Cinq-Mars D., Guay F., Raymond F., Dalmau A., Saucier L. 2023. Preslaughter feed withdrawal time and its effect on rabbit blood measures, gastrointestinal tract parameters and Longissimus lumborum glycolytic potential. World Rabbit Sci., 31: 237-261. https://doi.org/10.4995/wrs.2023.19177
Lazzaroni C., Biagini D., Lussiana C. 2009. Different rearing systems for fattening rabbits : Performance and carcass characteristics. Meat Sci., 82: 200-204. https://doi.org/10.1016/j.meatsci.2009.01.011
Lê S., Josse J., Husson F. 2008. FactoMineR: An, R Package for Multivariate Analysis. J. Stats. Soft., 25: 1-18. https://doi.org/10.18637/jss.v025.i01
Lebas F., Coudert P., Rouvier R., de Rochambeau H. 1997. The rabbit: husbandry, health and production. In: FAO (ed). Animal Production and Health Series, Rome, Italy.
Lee D.K. 2020. Data transformation: a focus on the interpretation. Korean. J. Anesthesiol., 73: 503-508. https://doi.org/10.4097/kja.20137
Leheska J.M., Wulf D.M., Maddock R.J. 2002. Effects of fasting and transportation on pork quality development and extent of postmortem metabolism. J. Anim. Sci., 80: 3194-3202. https://doi.org/10.2527/2002.80123194x
MAPAQ. 2019. Lignes directrices et normes pour l’interprétation des résultats analytiques en microbiologie alimentaire. Ministère de l’Alimentation, des Pêcheries et de l’Agriculture du Québec. https://www.mapaq.gouv.qc.ca/fr/Publications/recueil.pdf
Martín-Peláez S., Martín-Orúe S.M., Pérez J.F., Fàbrega E., Tibau J., Gasa J. 2008. Increasing feed withdrawal and lairage times prior to slaughter decreases the gastrointestinal tract weight but favours the growth of cecal Enterobacteriaceae in pigs. Livest. Sci., 119: 70-76. https://doi.org/10.1016/j.livsci.2008.02.012
Martín-Peláez S., Peralta B., Creus E., Dalmau A., Velarde A., Pérez J.F., Mateu E., Martín–Orúe S.M. 2009. Different feed withdrawal times before slaughter influence caecal fermentation and faecal Salmonella shedding in pigs. Vet. J., 182: 469-473. https://doi.org/10.1016/j.tvjl.2008.08.002
Masoero G., Riccioni L., Bergoglio G., Napoletano F. 1992. Implications of fasting and of transportation for a high quality rabbit meat product. In Proc.: 5th World Rabbit Congress, 25-30 July 1992, Corvallis, USA, 841-847.
McLellan M.R., Lind L.R., Kime R.W. 1995. Hue angle determinations and statistical analysis for multiquadrant Hunter, L, a, b data. J. Food Quality, 18: 235-240. https://doi.org/10.1111/j.1745-4557.1995.tb00377.x
Monin G., Sellier P. 1985. Pork of low technological quality with a normal rate of muscle pH fall in the immediate post-mortem period: The case of the Hampshire breed. Meat Sci., 13: 49-63. https://doi.org/10.1016/S0309-1740(85)80004-8
Møretrø T., Langsrud S., Heir E. 2013. Bacteria on meat abattoir process surfaces after sanitation: Characterisation of survival properties of Listeria monocytogenes and the commensal bacterial flora. Adv. Microbiol., 03: 255-264. https://doi.org/10.4236/aim.2013.33037
Nakyinsige K., Sazili A.Q., Aghwan Z.A., Zulkifli I., Goh Y.M., Fatimah A.B. 2013. Changes in blood constituents of rabbits subjected to transportation under hot, humid tropical conditions. Asian-Australas. J. Anim. Sci., 26: 874-878. https://doi.org/10.5713/ajas.2012.12652
Nattress F.M., Murray A.C. 2000. Effect of antemortem feeding regimes on bacterial numbers in the stomachs and ceca of pigs. J. Food Prot., 63: 1253-1257. https://doi.org/10.4315/0362-028X-63.9.1253
Newton K.G., Gill C.O. 1981. The microbiology of DFD fresh meats: A review. Meat Sci., 5: 223-232. https://doi.org/10.1016/0309-1740(81)90005-X
Pathare P.B., Opara U.L., Al-Said F.A.J. 2013. Colour measurement and analysis in fresh and processed foods: A review. Food Biopro. Technol., 6: 36-60. https://doi.org/10.1007/s11947-012-0867-9
Petracci M., Bianchi M., Biguzzi G., Cavani C. 2010. Preslaughter risk factors associated with mortality and bruising in rabbits. World Rabbit Sci., 18: 219-228. https://doi.org/10.4995/wrs.2010.781
Piattoni F., Demeyer D., Maertens L. 1997. Fasting effects on in vitro fermentation pattern of rabbit caecal contents. World Rabbit Sci., 5: 23-26. https://doi.org/10.4995/wrs.1997.314
Piles M., Blasco A., Pla M. 2000. The effect of selection for growth rate on carcass composition and meat characteristics of rabbits. Meat Sci., 54: 347-355. https://doi.org/10.1016/S0309-1740(99)00109-6
Pla M. 2008. A comparison of the carcass traits and meat quality of conventionally and organically produced rabbits. Livest. Sci., 115: 1-12. https://doi.org/10.1016/j.livsci.2007.06.001
Pla M., Cervera C. 1997. Carcass and meat quality of rabbits given diets having a high level of vegetable or animal fat. Anim. Sci., 65: 299-303. https://doi.org/10.1017/S1357729800016611
Pla M., Guerrero L., Guardia D., Oliver M.A., Blasco A. 1998. Carcass characteristics and meat quality of rabbit lines selected for different objectives: I. Between lines comparison. Livest. Prod. Sci., 54: 115-123. https://doi.org/10.1016/S0301-6226(97)00179-6
R Core Team. 2017. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing.
Rasmussen A.J., Anderson M. 1996. New method for determination of drip loss in pork muscles. In Proc.: 42nd International Congress of Meat Science and Technology, 1996. Lillehammer, Norway. 286-287.
Ribeiro J., Andrade E., Monteiro D., Pinheiro V. 2021. Effect of a feed restriction and gender on the performance and characteristics of the rabbit carcass in the fattening period. In Proc.: 12th World Rabbit Congress, 3-5 November 2021, Nantes, France, Q-18.
Rocha L.M., Dionne A., Saucier L., Nannoni E., Faucitano L. 2015. Hand-held lactate analyser as a tool for the realtime measurement of physical fatigue before slaughter and pork quality prediction. Animal., 9: 707-714. https://doi.org/10.1017/S1751731114002766
Saucier L., Gendron C., Gariépy C. 2000. Shelf life of ground poultry meat stored under modified atmosphere. Poult. Sci., 79: 1851-1856. https://doi.org/10.1093/ps/79.12.1851
Saucier L., Bernier D., Bergeron R., Méthot S., Giguère A., Faucitano L. 2007. Effect of feed texture, meal frequency and pre-slaughter fasting on behaviour, stomach weight and microbial carcass contamination in pigs. Can. J. Anim. Sci., 87: 479-486. https://doi.org/10.4141/A06-072
Składanowska-Baryza J., Ludwiczak A., Pruszyńska-Oszmałek E., Kołodziejski P., Bykowska M., Stanisz M. 2018. The effect of transport on the quality of rabbit meat. Anim. Sci. J., 89: 713-721. https://doi.org/10.1111/asj.12966
Trocino A., Xiccato G., Queaque P.I., Sartori A. 2003. Effect of transport duration and gender on rabbit carcass and meat quality. World Rabbit Sci., 11: 32-43. https://doi.org/10.4995/wrs.2003.494
Trocino A., Zomeño C., Birolo M., Di Martino G., Stefani A., Bonfanti L., Bertotto D., Gratta F., Xiccato G. 2018. Impact of pre-slaughter transport conditions on stress response, carcass traits, and meat quality in growing rabbits. Meat Sci., 146: 68-74. https://doi.org/10.1016/j.meatsci.2018.07.035
Verga M., Luzi F., Petracci M., Cavani C. 2009. Welfare aspects in rabbit rearing and transport. Ital. J. Anim. Sci., 8: 191-204. https://doi.org/10.4081/ijas.2009.s1.191
Vernay M., Raynaud P., Salse A. 1975. Répartition des acides gras volatils dans le tube digestif du lapin domestique. II. Lapins soumis au jeûne. Ann. Rech. Vétér., 6: 369-377.
Viljoen H.F., de Kock H.L., Webb E.C. 2002. Consumer acceptability of dark, firm and dry (DFD) and normal pH beef steaks. Meat Sci., 61: 181-185. https://doi.org/10.1016/S0309-1740(01)00183-8
Xiong G.Y., Xu X.L., Zhu X.B., Zhou G.H., Shi S. 2008. Effects of withholding food and/or water supply on the quality of meat from Rex rabbits. J. Muscle Foods., 19: 374-384. https://doi.org/10.1111/j.1745-4573.2008.00123.x
