Embryo recovery under anaesthesia after hCG or GnRH treatments in the rabbit and survival when a reduced number of embryos is transferred

 Marina López, F. Forcada, J.A. Rodríguez, María Martín, L. Zaragaza

Abstract

Ovulation rate and number of 72h post-mating recovered embryos were studied in nulliparous Spanish Giant does treated with an intravenous injection of 25 IU hCG (n=22) or 20 µg GnRH (n=20) intramuscularly after coitus. Embryos were recovered under anaesthesia by separata perfusion of the oviducts and uteri. One of the GnRH-treated does did not ovulate. No significant differences between groups were found either in the ovulation rate or in the number on recovered embryos per donor doe (8.5 ± 0.2 and 7.3 ± 0.2 vs 8.6 ± 0.4 and 6.8 ± 0.9 respectively for hCG and GnRH treated animals). After transfer into the uteri of synchronized Solaf commercial hybrid recipients, the lower number of embryos transferred per doe (5.9 vs 11.2) did not affect to the percentage of pregnant does (67 vs 75 % respectively), but increased the percentage of young born per pregnant doe (59 vs 41 % respectively) (P<0.05). Plasma progesterone levels in the recipients were higher in pregnant does (at least P<0.05) from Day 9 post-mating to the last blood sample recovered (Day 21), although the progesterone pattern in the non-pregnant ones indicated that the induction of ovulation was effective.

The results indicate the adequate embryo recovery rates obtained from Spanish Giant does under anaesthesia and the good efficiency after transfer of a reduced number of embryos per recipient doe.


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References

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 Universitat Politècnica de València

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