Impact of royal jelly to improve reproductive performance of male rabbits under hot summer conditions

A. M. El-Hanoun, A. E. Elkomy, W. A. Fares, E. H. Shahien

Abstract

To alleviate the deleterious effect of heat stress during summer conditions on male rabbits’ reproduction, 40 V Line adult rabbit bucks (on av. 8 mo old) were divided into 4 experimental groups and exposed to temperatures ranging from 23 to 36°C. Bucks in the 1st, 2nd, 3rd, and 4th group were supplemented with 0, 50, 100 or 150 mg of Chinese royal jelly (RJ)/kg twice per week, respectively, over a 20-wk period. Semen quality and blood biochemical constituents were evaluated. RJ at any dose exhibited a significant increase (P<0.05) in rabbits’ sperm concentration, total sperm output, sperm motility, live sperm and normal sperm compared to the untreated controls. Plasma total protein, albumin, globulin, glucose and high density lipids (HDL) concentrations were significantly (P<0.05) boosted in the RJ groups compared to the controls. In contrast, RJ treatment resulted in a significant (P<0.05) reduction in plasma total lipids, triglycerides, cholesterol and low density lipids (LDL) concentrations. Treatment with RJ significantly boosted (P<0.05) testosterone concentration in the RJ groups to reach 110, 120 and 128%, respectively, of the control group. Improved kidney and liver functions were observed in the RJ bucks groups where plasma creatinine, urea concentrations, aspartate aminotransferase and alanine aminotransferase enzyme activities were significantly (P<0.05) decreased by RJ treatments. Treating bucks subjected to heat stress by different RJ doses increased (P<0.05) total antioxidant capacity to 106, 111 and 115% of basal, but significantly reduced (P<0.05) malondialdehyde and thiobarbituric acid reactive substances compared to the untreated. It was concluded that Chinese royal jelly supplementation for heat-stressed male rabbits can counteract summer infertility and improve their physiological status.


Keywords

rabbit; heat stress; royal jelly; litter size; blood constituents

Full Text:

PDF

References

Abou-Hozaifa B.M., Badr El-Din, N.K. 1995. Royal jelly, a possible agent to reduce the nicotine-induced atherogenic lipoprotein profile. Saudi Med. J., 16: 337-342.

Al-Mufarrej S.I, El-Sarag M.S.A. 1997. Effects of royal jelly on the humoral antibody response and blood chemistry of chickens. J. Appl. Anim. Res., 12: 41-47. doi:10.1080/09712119.1997.9706186

Beutler E., Duron O., Kelly B.M. 1963. An improved method for the detection of blood glutathione. J. Lab. Clin. Med., 61: 882-888.

Blom E. 1950. A one-minute live-dead sperm stain by means of eosin-nigrosin. J. Fertil. Steril., 1: 176-177.

Crenguţa I.P., Liviu A.l.M., Otilia B., Daniel S.D., Agripina Ş., Ion R., Mariana N.M. 2011. Biological Activities of Royal Jelly-Review. Anim. Sci. Biotech., 44: 108-118.

Chiu D.T.Y., Stults F.H., Tappel A.L. 1976. Purification and properties of rat lung soluble glutathione peroxidase. Biochim. Biophys. Acta, 445: 558-566. doi:10.1016/0005-2744(76)90110-8

Conti M., Morand C., Levillain P., Lemmonnier A. 1991. Improved fluorometric determination of malondialdehyde. Clin. Chem., 37: 1273-1275.

Destrem H. 1956. Expérimentation de la gelée royale d’abeille en pratique gériatrique (134 cas). Rev. Franc. Geront., 3.

El-Banby M.A. 1987. Honey bees in koran and in medicine. Al-Ahram center for Translation and publication. Cairo. Egypt. 205 pp.

Elnagar S.A., Elghalid O.A., Abd-Elhady A.M. 2010. Royal jelly: can it reduce physiological strain of growing rabbits under Egyptian summer conditions? Animal, 4: 1547-1552. doi:10.1017/S1751731110000753

Elnagar S.A. 2010. Royal jelly counteracts bucks’“summer infertility”. Anim. Reprod.Sci., 121: 174-180. doi:10.1016/j.anireprosci.2010.05.008

El-Nekeety A.A., El-Kholy W., Abbas N.F., Ebaid A., Amra H.A., Abdel-Wahhab M.A. 2007. Efficacy of royal jelly against the oxidative stress of fumonisin in rats. Toxicon, 50: 256-269. doi:10.1016/j.toxicon.2007.03.017

Erel O. 2004. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin. Biochem., 37: 112-119. doi:10.1016/j.clinbiochem.2003.10.014

Faisal B.A., Abdel-Fattah S.A., El-Hommosany Y.M., Abdel-Gawad N.M., Ali M.F.M. 2008. Immunocompetence, hepatic heat shock protein 70 and physiological response to feed restriction and heat stress in two body weight lines of Japanese quail. Int. J. Poultry Sci., 7: 174-183. doi:10.3923/ijps.2008.174.183

Fujihara N., Koga O., Nishiyama H. 1983. Secretory function of the ductus deferens of male chickens. Poultry Sci., 62: 557-560. doi:10.3382/ps.0620557

Graham J. 1992. The hive and the honey bee. revised edition. Dadant and Sons, Hamilton, Illinois, USA.

Guo H., Saiga A., Sato M., Miyazawa I., Shibata M., Takahata Y., Morimatsu F. 2007. Royal jelly supplementation improves lipoprotein metabolism in humans. J. Nutr. Sci. Vitaminol., 3: 345-348. doi:10.3177/jnsv.53.345

Habig W.H., Pabst M.J., Jakoby W.B. 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249: 7130-7139.

Hang G., Ekusa A., Iwai K., Yonekura M., Takahata Y., Morimatsu F. 2008. Royal jelly peptide inhibit lipid peroxidation in vitro and in vivo. J. Nutr. Sci. Vitaminol., 54: 191-195. doi:10.3177/jnsv.54.191

Hidaka F., Matsuo S., Muta T., Takeshige K., Mizukami T., Nunoi H. 2006. A missense mutation of the Toll-like receptor 3 gene in a patient with influenza-associated encephalopathy. Clin. Immunol. 119: 188-194. doi:10.1016/j.clim.2006.01.005

Inoue S., Koya-Miyata S., Ushio S., Iwaki K., Ikeda M., Kurimoto M. 2003. Royal jelly prolongs the life span of C3H/HeJ mice: correlation with reduced DNA damage. Exp. Gerontol., 38: 965-969. doi:10.1016/S0531-5565(03)00165-7

International Rabbit Reproduction Group. IRRG 2005. Recommendations and guidelines for applied reproduction trials with rabbit does. World Rabbit Sci., 13: 147-164. doi:10.4995/wrs.2005.521

Kamel K.I., Elkomy A.E., El-Sbeiy M.E. 2009. The Androgenic Action of Gibberellic Acid (GA)3 on Reproductive Performance of New Zealand White Rabbit Bucks. World J. Agric. Sci., 5:40-48.

Karacal F., Aral F. 2008. Effect of the royal jelly on sperm quality in mice. Indian Vet. J., 85: 331-332.

Kohguchi M., Inoue S., Ushio S., Iwaki K., Ikeda M., Kurimoto M. 2004. Effect of royal jelly diet on the testicular function of hamsters. Food Sci. Tech. Res., 10: 420-423. http://dx.doi.org/10.3136/fstr.10.420

Kurkure N.V., Pawar S.P., Kognole S.M., Bhandarkar A.G., Ganorkar A.G., Kalorey D.R. 2000. Ameliorative effect of turmeric (Curcuma longa) in induced aflatoxicosis in cockerels. Ind. J. Vet. Pathol., 24: 26-28.

Lewis, R. 2005. The infertility cure: the ancient Chinese wellness program for getting pregnant and having healthy babies. ed. Little, Brown and Company.

Mărghitaş, L.A. 2008. Produsele apicole şi principalele lor însuşiri terapeutice. In: Albinele şi produsele lor. L.A. Mărghitaş, 2nd ed., Ceres, Bucharest, Romania. 280-378.

Misra H.P., Fridovich I. 1972. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem., 247: 3170-3175.

Moritz R.F.A., Southwick E.E. 1992. Bees a superorganisms. An evolutionary reality. 1st ed., Springer-Verlag, Berlin, Germany. doi:10.1007/978-3-642-84666-3

Münstedt, K., Henschel M., Hauenschild A., von Georgi R. 2009. Royal jelly increases high density lipoprotein levels but in older patients only, J. Altern. Complem. Med., 15: 329-330. doi:10.1089/acm.2008.0420

Pizzorno J.E., Murray M.T., Joiner-Bey H. 2007. The Clinician’s Handbook of Natural Medicine, 2nd ed. Churchill Livingstone, Elsevier, Philadelphia, USA.

Prescott J., Lastra M., Barksdale L. 1982. Equi factors in the identification of Corynebacterium equi Magnusson. J. Clin. Microb., 16: 988-990.

SAS, 2002. SAS/STAT User's guide statistics. SAS institute INC., Cary. NC, USA.

Schultz L.A. 1987. Methods in Clinical Chemistry. The C.V. Mosby Co. St Louis, USA, 742-746.

Shinoda M., Nakajin S., Oikawa T., Sato K., Kamogawa A., Akiyama Y. 1978. Biochemical studies on vasodilative factor in royal jelly. Yakugaku Zassii, 98: 139-145.

Smith J.T., Mayer D.T. 1955. Evaluation of sperm concentration by the hemocytometer method. Fertil Steril, 6: 271-275.

Vittek J. 1995. Effect of royal jelly on serum lipids in experimental animals and humans with atherosclerosis, Experientia, 51: 927-935. doi:10.1007/BF01921742

Vucevic D., Melliou E., Vasilijic S., Gasic S., Ivanovski, P., Chinou I., Colic M. 2007. Fatty acids isolated from royal jelly modulate dendritic cell-mediated immune response in vitro. Int. Immunopharmacol., 7: 1211-1220. doi:10.1016/j.intimp.2007.05.005

Xu B., Zeng Z. 2011. Effect of royal jelly on growth and reproduction performance of male rabbits. Scientia Agricultura Sinica, 10: 2154-2160.

Yamada K., Ikeda I., Maeda M., Shirahata S., Murakami H. 1990. Effect of immunoglobulin production stimulating factors in foodstuffs on immunoglobulin production of human lymphocytes. Agr. Biol. Chem. Tokyo, 54: 1087-1089. doi:10.1271/bbb1961.54.1087

Abstract Views

8591
Metrics Loading ...

Metrics powered by PLOS ALM


 

Cited-By (articles included in Crossref)

This journal is a Crossref Cited-by Linking member. This list shows the references that citing the article automatically, if there are. For more information about the system please visit Crossref site

1. Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits
Visweswara Rao Pasupuleti, Lakhsmi Sammugam, Nagesvari Ramesh, Siew Hua Gan
Oxidative Medicine and Cellular Longevity  vol: 2017  first page: 1  year: 2017  
doi: 10.1155/2017/1259510



 

 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs