Production and characterization of gelatin from rabbit bone as bioplastics material by acid pre-treatment




Keywords, Bioplastic, gelatin, rabbit bone


This study aims to find out the effect of hydrochloric acid curing concentration on the characteristics of rabbit bone gelatin and determine the level of hydrochloric acid concentration for the soaking process to produce the best characteristics of rabbit bone gelatin. The material used was 50 kg of Rex rabbit bones obtained from rabbit farms, HCl 4, 5 and 6% and distilled water. The rabbit skin was soaked in hydrochloric
acid (4, 5 and 6%) for 4 d as treatment and replicated three times. Gelatin extraction was performed three times at temperatures of 65, 75 and 85°C for 4 h each time and the results obtained were filtered through filter paper. The filtrate was concentrated at 50°C for 5 h. The concentrated filtrate was then poured into a tray before drying in an oven at 50°C until dry. Milling was carried out until it became gelatin powder. This study used a completely randomised design with a unidirectional pattern, and if there was a significant difference, continued with Duncan’s multiple range test. The results showed that the rabbit bone gelatin yield was between 6.18-8.52%, moisture 8.08-8.45%, ash content 8.15-10.93%, pH 3.85-4, protein content 57.09-62.84%, fat content 0.04-0.27%, gel strength 74.47-129.09 bloom, viscosity 3.06-4.26 cP, thick point 10-12°C, melting point 33-35°C and the molecular weights were 85, 120, and 212.5 kDa. The characteristics of rabbit bone gelatin still meet the Standar Nasional Indonesia gelatin range. Curing treatment with 6% HCl gave the best gelatin characteristics.


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Author Biographies

Dwi Wulandari, Polytechnic ATK Yogyakarta

Department of Leather Processing Technology

Indri Hermiyati, Polytechnic ATK Yogyakarta

Department of Plastic Rubber Processing Technology

Iswahyuni Iswahyuni, Polytechnic ATK Yogyakarta

Department of Plastic Rubber Processing Technology

Armila Zahra Tawarniate, Polytechnic ATK Yogyakarta

Department of Leather Processing Technology


Ahmad M., Benjakul S. 2011. Characteristics of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as influenced by acid pretreatment and extraction time. Food Hydrocoll., 25: 381-388.

AOAC. 2012. Official Methods of Analysis of AOAC International. In Association of Official Analysis Chemists International. Arlington: Association of Official Analytical Chemists Inc.

Atma Y., Ramdhani H., Mustopa A.Z., Pertiwi M., Maisarah R. 2018. Physicochemical characteristics of catfish bone gelatin (Pangasius sutchi ) extracted using pineapple waste (Ananas comosus). Agritech., 38: 56-63.

Bahar A., Kusumawati N., Anggrani M.A., Muslim S. 2018. Optimization of curing and extraction time on the production of base gelatin from bovine skin. Material, 112: 58-62. (Iconhomecs 2017),.

British Standard 757. 1975. Sampling and Testing of Gelatin. In Imeson: Thikcening and Gelling Agents for Food. 1992. Academic Press, New York.

Choi S.S., Regenstein J.M. 2000. Physicochemical and sensory characteristics of fish gelatin. J. Food Sci,. 65: 194-199.

Darwin D., Ridhay A., Hardi J. 2018. Study of extracting gelatin from tilapia fish bones (Oreochromis mossambicus). Kovalen: J. Ris. Kim., 4: 1-15.

Eysturskard J., Haug I.J., Elharfaoui N., Djabourov M., Draget K. I. 2009. Structural and mechanical properties of fish gelatin as a function of extraction conditions. Food Hydrocoll., 23: 1702-1711.

Food Chemicals Codex. 1996. Food and Nutrition Board, National Academy of Sciences. 4th ed. Washington DC: National Academy Press.

Gelatin Manufacturers Institut of America. 2012. Gelatin.

Giménez B., Gómez-Guillén M. C., Montero P. 2005. Storage of dried fish skins on quality characteristics of extracted gelatin. Food Hydrocoll., 19: 958-963.

Hafsari A.R., Rosmiati D., Jamaludiin D. 2018. The effect of hydrochloric acid (HCl) concentration on the quality of gourami bone gelatin (Ospheronemus Gouramy Lac). In Proc.: 1st International Conference on Recent Innovations (ICRI): 2983-2989.

Hakim T.R., Pratiwi A., Jamhari., Fitriyanto N.A., Rusman, Abidin M.Z., Matulessy D.N., Erwanto Y. 2021. Extraction of collagen from the skin of kacang goat and production of its hydrolysate as an inhibitor of angiotensinconverting enzyme. Trop. Anim. Sci. J., 44: 222-228.

Hardikawati T., Puspawati N.M., Ratnayani K. 2016. Study of the effect of variations in the concentration of citric acid on the gel strength of broiler skin gelatin products associated with the protein pattern. J. Kim., 10: 115-124.

Herniawati. 2008. Minerals and Homeostasis. Biology Education Department, Faculty of Mathematics and Natural Sciences. Indonesian Education University. Bandung. Available at Accessed March 2022.

Huda W., Atmaka W., Nurhartadi E. 2013. Study of physical and chemical characteristics of chicken leg bone extract gelatin (Gallus gallus bankiva) with variations in soaking time and acid concentration. J. Teknosains Pangan., 2: 70-75.

Jannah A., Maunatin A., Windayanti A., Findianti Y., Mufidah Z. 2013. Isolation and characterization of gelatin from chicken bones using the acid method. Alchemy., 2: 184-189.

Karim A.A, Bhat R. 2009. Review of Fish Gelatin: Properties. Challenges and prospects as an alternative to mammalian gelatins. Trends Food Sci. Technol., 19: 644-656.

Khirzin M. H., Ton S., Fatkhurrohman F. 2019. Extraction and characterization of duck bone gelatin using the acid extraction method. J. Sain Peternak Indones., 14: 119-127.

Laemmli U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.

Liu F., Chiou B. Sen, Avena-Bustillos R.J., Zhang Y., Li Y., McHugh T.H., Zhong F. 2017. Study of combined effects of glycerol and transglutaminase on properties of gelatin films. Food Hydrocoll., 65: 1-9.

Mahmoodani F., Ardekani V.S., See S.F., Yusop S.M., Babji A.S. 2014. Optimization and physical properties of gelatin extracted from pangasius catfish (Pangasius sutchi) bone. J. Food Sci. Technol., 51: 3104-3113.

Miskah S., Ramadianti I.M., Hanif A.F. 2010. Effect of concentration of H3COOH & HCl as solvent and immersion time on the manufacture of gelatin made from chicken leg bone/skin. J. Tek. Kim., 17: 1-6. Available at Accessed March 2022.

Mulyani S., Setyabudi F.S.M.C.S., Pranoto Y., Santoso U. 2017. Physicochemical properties of gelatin extracted from buffalo hide pretreated with different acids. Korean J. Food Sci. Anim. Resour., 37: 708-715.

Mohan A., Long J. M. 2021. Chapter 23 - Valorization of wastes and by-products from the meat industry. In: Rajeev Bhat (Ed).Valorization of Agri-Food Wastes and By-Products. Academic Press, pp. 457-474.

Nuryanto R., Trisunaryanti W., Falah I.I., Triyono. 2018. Extraction of gelatin from catfish bone using NaOH and its utilization as a template on mesoporous silica-alumina. IOP Conference Series: Materials Science and Engineering., 349: 1-7.

Panjaitan T.F.C. 2016. Optimization of gelatin extraction from tuna fish bones (Thunnus albacares). J. Wiyata., 3: 11-16. Available at Accessed March 2022.

Permata Y., Widiastri F., Sudaryanto Y., Anteng A. 2016. Gelatin from catfish bones (Clarias batrachus): Preparation by acid method, characterization and application as thickener in syrup industry. J. Ilm Widya Tek., 15: 146-152.

Pertiwi M., Atma Y., Mustopa A., Maisarah R. 2018. Physical and chemical characteristics of gelatin from catfish bones with citric acid pre-treatment. J. Apl. Teknol. Pangan., 7: 83-91.

Petracci M., Cavani C. 2013. Rabbit meat processing: historical perspective to future directions. World Rabbit Sci., 21: 217-226.

Petracci M., Soglia F., Baldi G., Balzani L., Mudalal S., Cavani C. 2018. Technical note: estimation of real rabbit meat consumption in italy. World Rabbit Sci., 26: 91-96.

Rahman M.N.A., Jamalulail S.A.S.K.A. 2012. Extractions, Physicochemical Characterizations and Sensory Quality of Chicken Feet Gelatin. Borneo Sci., 30: 1-13. Available at Accessed March 2022.

Said M.I., Triatmojo S., Erwanto Y., Fudholi A. 2012. Characteristics of goat skin gelatin that produced through acid and alkali process. Agritech: J. Fac. Agric. Technol. UGM., 31: 190-200.

Santoso C., Surti T., Sumardianto S. 2015. Differences in the use of the concentration of citric acid solution in the manufacture of stingray cartilage gelatin (Himantura gerrardi ). J. Pengolah. dan Bioteknol. Has. Perikan., 4: 106-114. Available at Accessed March 2022.

See S.F., Ghassem M., Mamot S., Babji A.S. 2015. Effect of different pretreatments on functional properties of African catfish (Clarias gariepinus) skin gelatin. J. Food Sci. Technol., 52: 753-762.

Sinaga R.F., Ginting G.M., Hasibuan R., Ginying M.H.S. 2014. The effect of additional glycerol on the properties of tensile strength and elongation at the break-up of bioplastics from taro tuber starch. J. Tek. Kim. USU., 3: 19-24. Available at Accessed March 2022.

Sinthusamran S., Benjakul S., Kishimura H. 2014. Characteristics and gel properties of gelatin from skin of seabass (Lates calcarifer) as influenced by extraction conditions. Food Chem., 152: 276-284.

SNI 06-3735. 1995. Gelatin Quality and Test Method. National Standardization Board. Jakarta

Steel R.G., Torrie J.H. 1993. Principle and Procedure of Statistic. Mc. Graw Hill Book Company. Inc New York.

Suryaningrum T.D., Utomo B.S.B. 2002. Instructions for Analysis of Seaweed and Its Processed Products. Fishery and Marine Product Processing and Socio-Economic Research Center, Jakarta.

Tazwir T., Ayudiarti D. L., Peranginangin R. 2014. Optimization of gelatin production from kaci-kaci fish bones (Plectorhynchus chaetodonoides Lac.) using various acid concentrations and extraction times. J. Pascapanen dan Bioteknol. Kelaut. dan Perikan., 2: 35-43.

Ward A.G., Courts. 2009. The Science and Technology of Gelatin. Digitized edition, Academy Press, New York.

Wardhani D.H., Rahmawati E., Arifin G.T., Cahyono H. 2017. Characteristics of demineralized gelatin from lizardfish (Saurida spp.) scales using NaOH-NaCl solution. J. Bahan Alam Terbarukan., 6: 132-142.

Yudhistira B., Palupi E., Atmaka W. 2019. The effect of acid concentration and duration of submersion toward the characteristics of gelatin of eel fishbone (Anguilla bicolor) produced through The acid process. IOP Conference Series: Earth Environ. Sci. 246: 1-7.

Zhou P., Regenstein J.M. 2005. Effect of alkaline and acid pretreatments on Alaska pollock skin gelatin extraction. J. Food Sci., 70: 992-396

Zulkifli M., Naiu A.S., Yusuf N. 2014. Yield, gel point, and the melting point of tuna bone gelatin processed with palm vinegar. J. Ilm. Perikan dan Kelaut., 2: 73-77.