Glutathione supplementation in semen extender improves rabbit sperm attributes during refrigeration




glutathione, refrigeration, sperm quality, rabbit


In the present study, we evaluated the sustaining effect of various glutathione (GSH) concentrations in extender on rabbit sperm attributes during storage at 5°C for 24 h. Semen was collected from regular donor rabbit bucks using an artificial vagina and initially evaluated for sperm quality. The qualifying ejaculates were diluted with one of the extenders having 0, 1, 2, 4 or 8 mM GSH, to achieve a final concentration of 1×108 sperm/mL. The extended samples were stored at 5°C for 24 h. Sperm motility, motion kinetics, acrosome integrity and viability were assessed after 3, 6, 12 and 24 h of storage. The results showed that total sperm motility and sperm motion kinetics (oscillation index of the sperm, straightness index and beat cross frequency) were influenced (P<0.05) by glutathione dose and refrigeration time. An interaction of (P<0.05) GSH concentrations and refrigeration time was observed for sperm viability and acrosome reaction rate. In conclusion, the 4 mM GSH supplemented extender’s protective influence was remarkable to maintainrabbit sperm quality for 24 h 5°C.


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Author Biographies

Ejaz Ahmad, Bahauddin Zakariya University

Department of Clinical Sciences, Faculty of Veterinary Sciences

Zahid Naseer, Pir Mehr Ali Shah Arid Agriculture University

Faculty of Veterinary and Animal Sciences

Melih Aksoy, Aydin Adnan Menderes University

Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine


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