The influence of porcine cathelicidins on neutrophils isolated from rabbits in the course of bone graft implantation

J. Wessely-Szponder; T. Szponder; R. Bobowiec; A. Smolira

doi:10.4995/wrs.2013.1350

Authors

J. Wessely-Szponder Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Poland
T. Szponder Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
R. Bobowiec Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine. University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.
A. Smolira Institute of Physics, Division of Molecular Physics, Maria Curie Sklodowska University, Lublin, Poland.

DOI:

https://doi.org/10.4995/wrs.2013.1350

Keywords:

cathelicidin, neutrophil, rabbit

Abstract

Antimicrobial peptides are important elements of host defence because of their direct antimicrobial activity and modulatory role in innate immune response. The purpose of the study was to determine whether porcine peptides PR-39, protegrins (PGs) and low molecular weight extract (LMWE) are able to influence the neutrophil response during bone graft implantation in rabbits. The study was conducted on 10 White New Zealand rabbits and neutrophil activity was assayed on the basis of elastase, myeloperoxidase, and alkaline phosphatase release as well as free radical generation. Our study showed that PR-39 and PGs inhibited enzyme release from neutrophils except for elastase, which is essential in the first phase of injury. Superoxide and nitric oxide generation under the influence of PR-39 and PGs were also decreased. Moreover, we found that unlike separated peptides PR-39 and PGs, LMWE acts proinflammatorily, intensifying the neutrophil secretory response and free radical generation. These results should be taken into account in treatment with natural antimicrobial peptides. The increased neutrophil responses in the first phase of inflammation during surgery may be useful in prevention of infection, but LMWE should not be used in conditions in which excessive neutrophil response is injurious.

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