Technical note: Residues of gaseous air pollutants in rabbit (Oryctolagus cuniculus) tissues

Authors

DOI:

https://doi.org/10.4995/wrs.2020.13175

Keywords:

gaseous air pollutant, residual pollutant, tissue, farm, Oryctolagus cuniculus, rabbit

Abstract

The modern consumer is concerned not only for meat quality, but also about animal welfare and the environment. Studies were conducted to determine the concentration of gaseous residues in the tissues of rabbits. For this purpose, gaseous air pollutants were measured at the height of rabbit cages. Immediately after slaughter, samples were taken for analysis to determine the level of residual pollutants in the tissues (blood, perirenal fat and lung). Headspace gas chromatography was performed on the tissue samples to test for volatile toxic substances. Gas residues of 11 compounds were determined in the samples of blood, perirenal fat and lungs. The same chemicals were present in the air of the farm and the animal tissues, which may indicate their capacity for bioaccumulation. We recommend that the results should be used to develop guidelines regarding the welfare of meat rabbits and requirements for laboratory rabbits.

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Author Biographies

Bożena Nowakowicz-Dębek, University of Life Sciences in Lublin

Department of Animal Hygiene and Environmental Hazards

Jacek Petkowicz, Medical University in Lublin

Laboratory Diagnostics Department

Grzegorz Buszewicz, Medical University in Lublin

Laboratory of Forensic Toxicology

Łukasz Wlazło, University of Life Sciences in Lublin

Department of Animal Hygiene and Environmental Hazards

Mateusz Ossowski, University of Life Sciences in Lublin

Department of Animal Hygiene and Environmental Hazards

References

Agency for Toxic Substances and Disease Registry (ATSDR) 2010. Toxicological Profile for Ethylbenzene. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, 82-103.

Amoore J.E., Hautala E. 1983. Odor as an aid to chemical safety: Odor thresholds compared with threshold limit values and volatilities for 214 industrial chemicals in air and water dilution. J. Appl. Toxicol., 3: 272-290. https://doi.org/10.1002/jat.2550030603

Belenguer A., Fondevila M., Balcells J., Abecia L., Lachica M., Carro M.D. 2011. Methanogenesis in rabbit caecum as affected by the fermentation pattern: in vitro and in vivo measurements. World Rabbit Sci., 1: 75-83. https://doi.org/10.4995/wrs.2011.826

Calvet S., Estellés F., Hermida B., Blumetto O., Torres A. 2008. Experimental balance to estimate efficiency in the use of nitrogen in rabbit breeding. World Rabbit Sci., 16: 205-211. https://doi.org/10.4995/wrs.2008.615

Caussy D., Gochfeld M., Gurzau E., Neagu C., Ruedel H. 2003. Lessons from case studies of metals: investigating exposure, bioavailability, and risk. Ecotoxicol. Environ. Saf., 56: 45-50. https://doi.org/10.1016/S0147-6513(03)00049-6

Christoph G.R., Malley L.A., Stadler J.C. 2003. Subchronic inhalation exposure to acetone vapor and scheduled controlled operant performance in male rats. Inhal. Toxicol. 15: 781-798. https://doi.org/10.1080/08958370390217846

Da Borso F., Chiumenti A., Mezzadri M., Teri F. 2016. Noxious gases in rabbit housing systems: Effects of cross and longitudinal ventilation. J. Agric. Eng., 47: 222-229. https://doi.org/10.4081/jae.2016.572

Dickson, R.P., Luks, A.M. 2009. Toluene toxicity as a cause of elevated anion gap metabolic acidosis. Respir. Care, 54: 1115-1117.

Dikshith T.S.S. 2013. Hazardous Chemicals: Safety Management and Global Regulations. CRC Press Taylor & Francis Group LLC., Boca Raton, Florida, U.S. https://doi.org/10.1201/b14758

DiVincenzo G.D., Yanno F.J., Astill B.D. 1973. Exposure of man and dog to low concentrations of acetone vapor. Am. Ind. Hyg. Assoc. J., 34: 329-336. https://doi.org/10.1080/0002889738506857

Elovaara E., Engström K., Vainio H. 1984. Metabolism and disposition of simultaneously inhaled m-xylene and ethylbenzene in the rat. Toxicol. Appl. Pharmacol., 75: 466-478. https://doi.org/10.1016/0041-008X(84)90183-2

Environmental Protection Agency (EPA) 2010. Inventory of U.S. greenhouse gas emissions and sinks: 1990-2008. U.S.

Environmental Protection Agency report No. EPA 430-R-10-006. Washington, U.S.

Geller I., Hartmann R.J., Randle S.R., Gause E.M. 1979. Effects of acetone and toluene vapors on multiple schedule performance of rats. Pharmacol. Biochem. Behav., 11: 395-399. https://doi.org/10.1016/0091-3057(79)90114-X

Gugołek A., Juśkiewicz J., Strychalski J., Zwoliński C, Żary-Sikorska E., Konstantynowicz M. 2017. The effects of rapeseed meal and legume seeds as substitutes for soybean meal on productivity and gastrointestinal function in rabbits. Arch. Anim. Nutr., 71: 311-326. https://doi.org/10.1080/1745039X.2017.1322796

Howard P.H. 1989. Handbook of environmental fate and exposure data for organic chemicals. Volume 1: Large production and priority pollutants. Lewis Publishers Inc., Chelsea, Michigan.

Huff, J., Chan, P., Melnick, R. 2010. Clarifying carcinogenicity of ethylbenzene. Regul. Toxicol. Pharmacol., 58: 167-169. https://doi.org/10.1016/j.yrtph.2010.08.011

Kawai T., Yasugi T., Mizunuma K., Horiguchi S., Iguchi H., Ikeda M. 1992. Curvi-linear relation between acetone in breathing zone air and acetone in urine among workers exposed to acetone vapor. Toxicol. Lett. 62: 85-91. https://doi.org/10.1016/0378-4274(92)90081-T

Konéab A.P., Desjardinsbc Y., Gosselinbc A., Cinq-Marsa D., Guaya F., Saucier L. 2019. Plant extracts and essential oil product as feed additives to control rabbit meat microbial quality. Meat Sci., 50: 111-121. https://doi.org/10.1016/j.meatsci.2018.12.013

Lauwerys, R., Bernard, A., Viau, C., Buchet, J.P. 1985. Kidney disorders and hematotoxicity from organic solvent exposure. Scand. J. Work Environ. Health., 11 Suppl 1: 83-90. https://doi.org/10.5271/sjweh.2238

Michl R., Hoy St. 1996. Results of continuous measuring of gases in rabbit keeping by using multigas-monitoring. Berl. Munch. Tierarztl. Wochenschr., 109: 340-343.

Nowakowicz-Dębek B., Buszewicz G., Chmielowiec-Korzeniowska A., Saba L., Bis-Wencel H., Wnuk W. 2007. Residues of volatile gaseous substances in the tissues of polar foxes. Med. Wet., 63: 688-691.

Nowakowicz-Dębek B., Łopuszyński W. 2004. Effects, of air pollution on changes in the polar fox (Alopex lagopus) organism. Med. Wet., 60: 845-848.

OECD SIDS. 2002. Ethylbenzene: SIDS Initial Assessment Report For SIAM 14. Paris, France: UNEP Publications 7, 1-177.

Ogata M., Fujisawa K., Ogino Y., Mano E. 1984. Partition coefficients as a measure of bioconcentration potential of crude oil compounds in fish and shellfish. Bull. Environ. Contam. Toxicol., 33: 561-567. https://doi.org/10.1007/BF01625584

Peckham, T., Kopstein, M., Klein, J., Dahlgren, J. 2014. Benzenecontaminated toluene and acute myeloid leukemia: a case series and review of literature. Toxicol. Ind. Health., 30: 73-81. https://doi.org/10.1177/0748233712451764

Plaa G.L., Hewitt W.R., Du Souich P., Caille G., Lock S. 1982. Isopropanol and acetone potentiation of carbon tetrachloride–induced hepatotoxicity: single versus repetitive pretreatments in rats. J. Toxicol. Environ. Health., 9: 235-250. https://doi.org/10.1080/15287398209530158

Rommers, J.M., de Jong, I.C., de Greef, K.H. 2015. The development of a welfare assessment protocol for commercially housed rabbits. In 19th International symposium on housing and diseases of rabbits, furbearing animals and pet animals, 27-28 May 2015, Celle, Germany.

Saghir, S.A., Rick, D.L., McClymont, E.L., Zhang, F., Bartels, M.J., Bus, J.S. 2009. Mechanism of ethylbenzene-induced mousespecific lung tumor: metabolism of ethylbenzene by rat, mouse, and human liver and lung microsomes. Toxicol. Sci., 107: 352-366. https://doi.org/10.1093/toxsci/kfn244

Scholl H.R., Iba M.M. 1997. Pharmacokinetics of and CYP1A induction by pyridine and acetone in the rat: interactions and effects of route of exposure. Xenobiotica, 27: 265-277. https://doi.org/10.1080/004982597240596

Tang W., Hemm I., Eisenbrand G. 2000. Estimation of human exposure to styrene and ethylbenzene. Toxicology, 144: 39-50. https://doi.org/10.1016/S0300-483X(99)00188-2

Tillmann K., Windschnurer I., Gamper J., Hinney B., Rülicke T., Podesser B.K., Troxler J., Plasenzotti R. 2019. Welfare assessment in rabbits raised for meat and laboratory purposes in enclosures with two floor types: Perforated plastic with holes versus slats. Res. Vet. Sci., 122: 200-209. https://doi.org/10.1016/j.rvsc.2018.11.016

Viau C. 2002. Biological monitoring of exposure to mixtures. Toxicol. Lett., 134: 9-16. https://doi.org/10.1016/S0378-4274(02)00158-3

Vitale, C.M., Gutovitz, S. 2018. Aromatic (Benzene, Toluene) Toxicity. In: StatPearls. StatPearls Publishing, Treasure Island (FL); 2019. Available at: https://www.ncbi.nlm.nih.gov/books/NBK532257. Accessed April 2020.

Wigaeus E., Holm S., Astrand I. 1981. Exposure to acetone: uptake and elimination in man. Scand. J. Work Environ. Health, 7: 84-94. https://doi.org/10.5271/sjweh.2561

Wigaeus E., Löf A., Nordqvist M. 1982. Distribution and elimination of 2-[14C]-acetone in mice after inhalation exposure. Scand. J. Work Environ. Health, 8: 121-128. https://doi.org/10.5271/sjweh.2486

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Published

2020-06-30

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Management