Preliminary evaluation of fenugreek (Trigonella foenum-graecum) seed gum as a potential prebiotic for growing rabbits in Tunisia: effects on in vivo faecal digestibility and in vitro fermentation

J. Zemzmi, L. Ródenas, E. Blas, H. Abdouli, T. Najar, J.J. Pascual


This study aims to determine the effect of dietary inclusion of fenugreek seed gum (FSG), rich in galactomannans, on nutrient apparent digestibility and caecal environment, as well as on in vitro caecal fermentation of Tunisian growing rabbits. Three experimental diets were formulated, including 0, 0.25 and 0.5% of FSG (FSG0, FSG0.25 and FSG0.5, respectively) for the in vivo trial and 0, 0.125, 0.25, 0.5 and 100% of FSG (FSG0, FSG0125, FSG0.25, FSG0.5 and FSG100, respectively) for the in vitro trial. In the in vivo trial, 45 weaned rabbits 31 d old (15 per treatment) were housed in individual cages until 94 d of age. Apparent digestibility coefficients were determined at two ages, from 38 to 41 and from 56 to 59 d old, and caecal traits were recorded after slaughtering. In the in vitro trial, the five experimental diets were incubated with a rabbit caecal inoculum. Gas production was measured and modelled until 72 h and the fermentation traits were measured. Apparent faecal digestibility coefficients of main nutrients and main caecal environment traits were not significantly affected by the dietary inclusion of FSG (P>0.05). However, animals fed with FSG showed lower caecal pH (–0.15; P<0.05) values. Regarding the in vitro fermentation, FSG100 increased asymptotic gas production (+11.25, P<0.001), sharpness of the switching characteristic of the profile (+1.98, P<0.001) and the maximum substrate degradation rate (RM) (+0.188, P<0.001), but decreasing the time after incubation at which half of the asymptotic amount of gas has been formed (–5.86, P<0.001) and at which RM occurs (–4.53, P<0.01). Likewise, FSG100 significantly decreased caecal pH (–1.035, P<0.001), lactic acid (–9.51, P<0.069) and N-NH3 concentrations (–12.81, P<0.001). Meanwhile, it increased the total volatile fatty acids (VFA) production (+43.15, P<0.001). Gradual dietary inclusion of FSG from 0 to 0.5% only significantly increased total VFA production in the caecum (+100 mmol/L per percentage point of FSG inclusion; P<0.05). In conclusion, FSG is highly and rapidly in vitro fermented by rabbit caecal bacteria. However, dietary inclusion of FSG up to 0.5%, might be insufficient to affect the apparent digestibility and fermentation profile of growing rabbits to a great extent.


rabbit; in vivo; in vitro; fenugreek seed gum; faecal digestibility; caecal fermentation

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Abad-Guamán R., Larrea-Dávalos J.A., Carabaño R., García J., Carro M.D. 2018. Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits. World Rabbit Sci., 26: 227-240.

AOAC. 2002. Official methods of analysis of the AOAC International. 17th ed. AOAC International., Gaithersburg, MD USA.

Bartley G.B., Hiltry M.D., Andreson B.D., Clairemont A.C., Maschke S.P. 1981. “Maple-syrup” urine odor due to fenugreek ingestion. New Engl. J. Med., 305: 467.

Batey I.L. 1982. Starch Analysis Using Thermostable Alpha-Amylases. Starch/Stärke, 34: 125-128.

Boletín Oficial del Estado. 2013. Real Decreto 53/2013, por el que se establecen las normas básicas aplicables para la protección de los animales utilizados en experimentación y otros fines científicos, incluyendo la docencia. BOE, 34: 11370-11421.

Bónai A., Szendrő Zs., Matics Zs., Fébel H., Kametler L., Tornyos G., Horn P., Kovács F., Kovács M. 2010. Effect of inulin supplementation and age on growth performance and digestive physiological parameters in weaned rabbits. World Rabbit Sci., 18: 121-129.

Borshchevskaya L.N., Gordeeya T.L., Kalinina A.N., Sineokii S.P. 2016. Spectrophotometric determination of lactic acid. J. Anal. Chem., 71: 755-758.

Bovera F., Marono S., Di Meo C., Piccolo G., Iannaccone F., Nizza A. 2010. Effect of mannanoligosaccharides supplementation on caecal microbial activity of rabbits. Animal., 4: 1522-1527.

Carabaño R., Villamide M.J., García J., Nicodemus N., Llorente A., Chamorro S., Menoyo D., García-Rebollar P., García-Ruiz A.I., De Blas J.C. 2009. New concepts and objectives for proteinamino acid nutrition in rabbits: a review. World Rabbit Sci., 17: 1-14.

Evans E., Jebelian V. 1982. Effects of age upon nutrient digestibility by fryer rabbits. J. Appl. Rabbit Res., 5: 8-9.

Falcão-E-Cunha L., Castro-Solla L., Maertens L., Marounek M., Pinheiro V., Freire J., Mourão J.L. 2007. Alternatives to antibiotic growth promoters in rabbit feeding: A review. World Rabbit Sci., 15: 127-140.

Fernández-Carmona J., Blas E., Pascual J.J., Maertens L., Gidenne T., Xicatto G., García, J. 2005. Recommendations and guidelines for applied nutrition experiments in rabbits. World Rabbit Sci., 13: 209-228.

Gidenne T. 1995. Effect of fibre level reduction and glucooligosaccharide addition on the growth performance and caecal fermentation in the growing rabbit. Anim. Feed Sci. Technol., 56: 253-263.

Gidenne T., Licois D. 2005. Effect of a high fibre intake on the resistance of the growing rabbit to an experimental inoculation with an enteropathogenic strain of Escherichia coli. Animal Sci., 80: 281-288.

Gómez-Conde M.S., Garcia J., Chamorro S., Eirás P., Rebollar P.G., De Rozas A.P., Badiola I., De Blas C., Carabaño R. 2007. Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old weaned rabbits. J. Ani. Sci., 85: 3313-3321.

Gómez-Conde M.S., Pérez de Rozas A., Badiola I., Pérez-Alba L., de Blas C., Carabaño R., García J. 2009. Effect of neutral detergent soluble fibre on digestion, intestinal microbiota and performance in twenty five day old weaned rabbits. Livest. Sci., 125: 192-198.

Groot J.C.J., Cone J.W., Williams B.A., Debersaques F.M.A., Lantinga E.A. 1996. Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feeds. Anim. Feed Sci. Technol., 64: 77-89.

Jiang J.X., Zhu L.W., Zhang W.M.,Sun R.C. 2007. Characterization of Galactomannan Gum from Fenugreek (Trigonella foenum graecum) Seeds and its rheological properties. Int. J. Polym. Mater. Po., 56: 1145-1154.

Jouany J.P. 1982. Volatile Fatty Acid and Alcohol Determination in Digestive Contents, Silage Juices, Bacterial Cultures and Anaerobic Fermentor Contents. Sciences des Aliments. 2: 131-144.

Korman S.H., Cohen E., Preminger A. 2001. Pseudo-maple syrup urine disease due to maternal prenatal ingestion of fenugreek. J. Pediatr. Child Health, 37: 403-404.

Koroleff F. 1966. Direct spectrophotometric determination of ammonia in precipitation. Tellus XVIII., 2.

Lipinski K., Purwin C., Tywonczuk J., Zdunczyk Z., Wroblewska M., Laniewska-Trokenheim L., Warminska-Radyko L. 2005. Effects of feed additives on nutrient digestibility and the bacterial status of faeces in pigs. J. Anim. Feed Sci., 14: 369-372.

Majeed M., Majeed S., Nagabhushanam K., Arumugam S., Natarajan S., Beede K., Furqan A. 2018. Galactomannan from Trigonella foenum-graecum L. Seed: Prebiotic application and its fermentation by prebiotic Bacillus coagulans strain MTCC 5856. Food Sci. Nutr., 6: 666-673.

Marten G.C., Barnes R.F. 1980. Prediction of energy digestibility of forages with in vitro rumen fermentation and fungal enzyme systems. In: W.J. Pigden, C.C. Balch, M. Graham (Eds.), In Proc.: The International Workshop on Standardization of Analytical Methodology for Feeds, Int. Dev. Res. Center, Ottawa, Ont., Canada. 61-71.

Martínez-Vallespín B., Martínez-Paredes E., Ródenas L., Cervera C., Pascual J.J., Blas E. 2011. Combined feeding of rabbit female and young: Partial replacement of starch with acid detergent fibre or/and neutral detergent soluble fibre at two protein levels. Livest. Sci., 141: 155-165.

Martínez-Vallespín B., Martínez-Paredes B., Ródenas L., Moya J.M., Cervera C., Pascual J.J., Blas E. 2013. Partial replacement of starch with acid detergent fibre and/or neutral detergent soluble fibre at two protein levels: Effects on ileal apparent digestibility and caecal environment of growing rabbits. Livest. Sci., 154: 123-130.

Mazza G., Di Tommaso D., Foti S. 2002. Volatile constituents of Sicilian fenugreek (Trigonella foenum-graceum L.) seeds. Sciences des Aliments, 22: 249-264.

Mertens D.R. 2002. Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: Collaborative study. J. AOAC Int., 85: 1217-1240.

Naidu M.M., Shyamala B.N., Naik J.P., Sulochanamma G., Srinivas P. 2011. Chemical composition and antioxidant activity of the husk and endosperm of fenugreek seeds. LWT-Food Sci. Technol., 44: 451-456.

Ocasio-Vega C., Abad-Guamán R., Delgado R., Carabaño R., Dolores Carro M., Javier García. 2018. Effect of cellobiose supplementation and dietary soluble fibre content on in vitro caecal fermentation of carbohydraterich substrates in rabbits. Arch. Anim. Nutr., 72: 221-238.

Perez J.M., Lebas F., Gidenne T., Maertens L., Xiccato G., Parigi-Bini R., Dalle Zotte A., Cossu M.E, Carazzolo A., Villamide M.J., Carabaño R., Fraga M.J., Ramos M.A., Cervera C., Blas E., Fernández J., Falcão-e-Cunha L., Freire J. 1995. European reference method for in vivo determination of diet digestibility in rabbits. World Rabbit Sci., 3: 41-43.

Read T., Gidenne T., Combes S., Labatut D., Bricard D., Bébin K., Fortun-Lamothe L. 2017. Digestibilité comparée chez le lapin: effets de l’âge, de l’état et du stade physiologiques. In Proc.: 17èmes Journées de la Recherche Cunicole, 21-22 Novembre, 2017. Le Mans, France. 117-180.

Robertson J. B., Van Soest P. J., James W. P. T., & Theander O. 1981. The analysis of dietary fiber in food. The detergent system of analysis and its application to human foods. James, WPT and Theander, O., eds. Marcel Dekker, New York, NY, 123-158.

Rosell J.M., de la Fuente L.F., Badiola J.I., Fernández de Luco D., Casal J., Saco M. 2009. Study of urgent visits to commercial rabbit farms in Spain and Portugal during 1997-2007. World Rabbit Sci., 17: 127 -136.

SAS. 2009. SAS/STAT User’s Guide (Release 9.2). SAS Institute. Inc., Cary NC, USA.

Schofield P. 2000. Gas production methods. Farm Animal Metabolism and Nutrition. J.P.F. D’Mello, ed. CAB International, Wallingford, Oxon, U.K. 209-232.

Sewell A. C., Mosandl A., Bohles H. 1999. False diagnosis of maple syrup urine disease owing to ingestion of herbal tea. Massachusetts Medical Society., 341: 769.

Skrivanova E., Marounek M., Benda V., Brezina P. 2006. Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringens to organic acids and monolaurin. Veterinární Medicina., 51: 81-88.

Skrivanova E., Molatova Z., Marounek M. 2008. Effects of caprylic acid and triacylglycerols of both caprylic and capric acid in rabbits experimentally infected with enteropathogenic Escherichia coli O. Vet. Microbiol., 126: 372-376.

Trocino A., Fragkiadakis M., Majolini D., Tazzoli M., Radaelli G., Xiccato G. 2013a. Soluble fibre, starch and protein level in diets for growing rabbits: Effects on digestive efficiency and productive traits. Anim. Feed Sci. Technol., 180: 73-82.

Trocino A., García J., Carabaño R., Xiccato G. 2013b. A meta-analysis of the role of soluble fiber in diets for growing rabbits. World Rabbit Sci., 21: 1-15.

Van Soest P.J., Robertson J.B., Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3597.

Volek Z., Marounek M., Skrivanova V. 2007. Effect of a starter diet supplementation with mannan-oligosaccharide or inulin on health status, caecal metabolism, digestibility of nutrients and growth of early weaned rabbits. Animal., 1: 523-530.

Volek Z., Marounek M. 2011. Dried chicory root (Cichorium intybus l.) as a natural fructan source in rabbit diet: effects on growth performance, digestion and caecal and carcass traits. World Rabbit Sci., 19: 143-150.

Zemzmi J., Mabrouki S., Abdouli H., Najar T. 2017. Preliminary characterization of fenugreek seed gum for use as prebiotic in rabbits’ nutrition. In Proc.: the 20th International Symposium on housing and diseases of rabbits, fur providing animals and pet animals, 17-18 May, 2017. Celle, Germany. 179-186.

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