Sodium butyrate in growing and fattening diets for early-weaned rabbits

Joana Ribeiro, Sandra Gaspar, Mário Pinho, João P. B. Freire, Luisa Falcão-e-Cunha

Abstract

To study the effect of adding coated sodium butyrate (SB) to growing-fattening rabbit diets, 2 trials were conducted. In trial 1, 180 rabbits were housed in pairs and fattened from 23 (weaning) to 63 d of age to evaluate their zootechnical performance. Trial 2 involved 30 rabbits, from 23 to 37 d of age and housed individually in digestibility cages, to evaluate digestibility, caecal fermentative activity and morphology of the intestinal mucosa. In both trials rabbits were randomly divided into 2 groups, each receiving one of the following diets: control diet [CTR, 360 g neutral detergent fibre (NDF) and 170 g crude protein (CP)/kg dry matter (DM)] and SB diet. The SB diet, similar to CTR diet, included coated SB at 5 g/kg by replacement of an identical quantity of wheat. In trial 1, after the first 2 wk, the SB content was reduced from 5 to 3 g/kg. In trial 2, faeces were collected over the last 6 d (32-37 d of age), with rabbits being slaughtered at 37 d of age. Gastric and caecal pH were measured and fermentative activity was determined in caecal contents. Three sections of the small intestine were excised from 20 rabbits (10 per treatment) for microscopic examination of intestinal villi and crypts in the proximal region, central region and distal region. In the first 2 wk after weaning, SB rabbits grew 8% less than their counterparts (P=0.002), but had a better feed conversion ratio (1.58 vs. 1.61; P=0.036). During the whole trial 1 period, SB improved feed conversion (P=0.005) and decreased feed intake (104.1 CTR vs. 98.8 g/d SB; P=0.017). No difference was recorded in daily weight gain (42.7 vs. 42.9 g/d). In both diets, the digestibility of DM, organic matter, energy, CP and NDF were similar. In the 3 intestinal regions of rabbits fed SB diet, crypts were deeper (P<0.05). There were no significant differences in villus height and width between treatments. Pectinase activity was higher (P=0.054) with SB diet, but cellulase and xylanase activity remained unaffected by diet. In our experimental conditions, the addition of SB allowed an improvement in feed conversion.


Keywords

coated Na-butyrate; performance; small intestine morphology; caecal activity; digestibility; rabbit

Full Text:

PDF

References

Abecia L., Fondevila M., Balcells J., Belenguer A. 2005. Effect of fumaric acid on diet digestibility and the caecal environment of growing rabbits. Anim. Res., 54: 493-498. https://doi.org/10.1051/animres:2005037

AOAC.1995. Official method 976.05 - Automated Kjeldahl method (Kjel-Foss Automatic). In: AOAC Official methods of analysis, 16th edition, vol. I, chapt. 4, p7.

Blank R., Mosenthin R., Sauer W.C., Huang S.1999. Effect of fumaric acid and dietary buffering capacity on ileal and fecal amino acid digestibilities in early-weaned pigs J. Anim. Sci. 77: 2974-2984. https://doi.org/10.2527/1999.77112974x

de Blas C., Mateos G. 1998. Feed formulation. In: de Blas C., Wiseman J. (ed) The nutriton of the rabbits. CABI International, Wallington, UK, 241-792.

Bünzen S. 2006. Capacidade tampão das rações para suínos. Boletim Técnico, Serrana Nutrição Animal.

Carraro L., Xiccato G., Trocino A., Radaelli G. 2005. Dietary supplementation of butyrate in growing rabbits. Ital. J. Anim. Sci., 4: 538-540. https://doi.org/10.4081/ijas.2005.2s.538

Cesari V., Toschi L., Pisoni A.M., Grilli G., Cesari N. 2008. Effect of dietary acidification on growth performance and caecal characteristic in rabbit. In Proc.: 9th World Rabbit Congress, 10-13 June, Verona, Italy, 583-587.

Falcão-e-Cunha L., Peres H., Freire J.P.B., Castro-Solla L. 2004. Effects of alfafa, wheat bran or beet pulp with or without sunfloweroil, on caecal fermentation and on digestibility in the rabbit. Anim. Feed Sci. Tech., 117:131-149. https://doi.org/10.1016/j.anifeedsci.2004.07.014

Falcão-e-Cunha L., Castro-Solla L., Maertens L., Marounek M., Pinheiro V., Freire J., Mourão J.L. 2007. Alternatives to antibiotic growth promoters in rabbit feeding: a review. World Rabbit Sci., 15: 127-140. https://doi.org/10.4995/wrs.2007.597

Gidenne T., Poncet C. 1985. Digestion,chez le lapin en croissance, d'une ration à taux élevé de constituants pariétaux: étude méthodologique pour le calcul de digestibilité apparente, par segment digestif. Ann. Zootech., 34: 429-446. https://doi.org/10.1051/animres:19850404

Gidenne T., Debray L., Fortun-Lamothe L., Le Huërou-Luron I. 2007. Maturation of the intestinal digestion and of microbiological activity in the young rabbit: impact of the dietaryfibre:starchratio.Comp. Biochem. Phys. A, 148: 834844. https://doi.org/10.1016/j.cbpa.2007.08.025

Gorka P., Kowalski Z. M., Pietrzak P., Kotunia A., Kiljanczyk R., Flaga J., Holst J. J., Guilloteau P., Zabielski R. 2009. Effect of sodium butyrate supplementation in milk replacer and starter diet on rumen development in calves. J. Physiol. Pharmacol., 60:47-53.

Guilloteau P., Zabielski R., David J.C., Blum J.W., Morisset J.A., Biernat M., Woliński J., Laubitz D., Hamon Y. 2009. Sodium-butyrate as a growth promotor in milk replacer formula for young calves. J. Dairy Sci., 92: 1038-1049. https://doi.org/10.3168/jds.2008-1213

Hu Z., Guo Y. 2007. Effects of dietary sodium butyrate supplementation on the intestinal morphological structure, absorptive function and gut flora in chickens. Anim. Feed Sci. Tech., 132:240-249. https://doi.org/10.1016/j.anifeedsci.2006.03.017

Hullar L., Fekete S., Szigeti G., Bokori J. 1996. Sodium butyrate as a natural growth promoter for rabbits. In Proc.: 6th World Rabbit Congress, 9-12 July. Toulouse, France, 175-179.

Jouany J.P. 1982. Dosage des acides gras volatils et des alcools dans les contenus digestifs, les Jus d'ensilage, les cultures bacterienne et les contenus de fermenteurs anaerobies. Sci. Aliment., 2: 131-144.

Kotunia A., Wolinski J., Laubitz D., Jurkowska M., Romé V., Guilloteau P., Zabielski R., 2004. Effect of sodium butyrate on small intestine development in neonatal piglets feed by artificial sow. J. Physiol. Pharmacol. 55: 59-68.

Michelan A.C., Scapinello C., Natali M.R., Furlan A.C., Sakaguti E.S., Faria H.G., Santolin M.L., Fernandes A.B. 2002. Utilização de probiótico, ácido orgânico e antibiótico em dietas para coelhos em crescimento: ensaio de digestibilidade, avaliação da morfometria intestinal e desempenho. Rev. Bras. Zootecn., 31: 2227-2237. https://doi.org/10.1590/S1516-35982002000900011

Mroz, Z. 2005. Organic acids as potencial alternatives to antibiotic growth promoters for pigs. Adv. Pork Prod., 16: 169-182.

Partanen K.H., Mroz Z., 1999. Organic acids for performance enhancement in pig diets. Nutrition Research Review, 12: 117-145. https://doi.org/10.1079/095442299108728884

Pluske J.R., Hampson D.J., Williams I.H., 1997. Factors influencing the structure and function of the small intestine in the weaned pig: a review. Lives. Prod. Sci., 51: 215-236. https://doi.org/10.1016/S0301-6226(97)00057-2

Romero C., Rebollar P.G., Dal Bosco A., Castellini C., Cardinali R. 2011. Dietary effect of short-chain organic acids on growth performance, mortality and development of intestinal lymphoid tissues in young non-medicated rabbits. World Rabbit Sci., 19: 133-142. https://doi.org/10.4995/wrs.2011.866

Salminen S., Bouley C., Boutron-Ruault M.C., Cummings J.H., Franck A., Gibson G.R., Isolauri E., Mores M.C., Roberfroid M., Rowland I., 1998. Functional food science and gastrointestinal physiology and function. Brit. J. Nutr. 80 (suppl.1): S147-S171. https://doi.org/10.1079/BJN19980108

SAS, 1991. SAS System for Linear Models 3rd edn. SAS institute Inc., Cary, NC.

Scapinello C., Faria H.G., Furlan A.C., Michelan A.C., 2001. Efeito do uso de oligossacarídeo manose e acidificantes sobre o desempenho de coelhos em crescimento. Revista Brasileira de Zootecnia, 30: 1275-1277. https://doi.org/10.1590/S1516-35982001000500021

Tung C.M., Pettigrew, J.E., 2006. Critical Review of acidifiers Report 05-169 America~s Pork Checkoff Program, University of Illinois.

Van Soest J.P., Robertson J.B., Lewis B.A., 1991. Methods for dietary fibre, neutral detergent fibre and non starch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3594. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Abstract Views

3731
Metrics Loading ...

Metrics powered by PLOS ALM


 

Cited-By (articles included in Crossref)

This journal is a Crossref Cited-by Linking member. This list shows the references that citing the article automatically, if there are. For more information about the system please visit Crossref site

1. Importance of release location on the mode of action of butyrate derivatives in the avian gastrointestinal tract
P.C.A. Moquet, L. Onrust, F. Van Immerseel, R. Ducatelle, W.H. Hendriks, R.P. Kwakkel
World's Poultry Science Journal  vol: 72  issue: 1  first page: 61  year: 2016  
doi: 10.1017/S004393391500269X



 

 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs