Morphology, chemical composition, and bacterial concentration of airborne particulate matter in rabbit farms

Elisa Adell, Fernando Estellés, Antonio G. Torres, María Cambra-López

Abstract

Livestock houses are major sources of airborne particulate matter (PM), which can originate from manure, feed, feathers, skin and bedding and may contain and transport microorganisms. Improved knowledge of particle size, morphology, chemical and microbiological composition of PM in livestock houses can help identify major sources of PM and contribute to the development of appropriate source-specific reduction techniques. In rabbit production systems, however, there is limited information on specific particle characteristics. The objective of this study was to characterise airborne PM in rabbit farms in terms of morphology, chemical compositions and bacterial concentration in different size fractions. Size-fractioned PM was sampled in the air of 2 rabbit farms, 1 for fattening rabbits and 1 for reproductive does, using a virtual cascade impactor, which simultaneously collected total suspended PM (TSP), PM10 and PM2.5 size fractions. Airborne PM samples were examined by light microscopy and scanning electron microscopy combined with energy dispersive X-ray analysis. Representative samples from potential sources of PM were also collected and examined. Additionally, a methodology to extract bacteria from the collected samples of airborne PM was developed to determine the bacterial concentration per PM size fraction. Results showed that airborne PM in rabbit farms is highly complex in particle morphology, especially in size. Broken skin flakes, disintegrated particles from feed or faecal material from mechanical fracture are the main sources of airborne PM in rabbit farms. Major elements found in rabbit airborne PM were S, Ca, Mg, Na and Cl. Bacterial concentrations ranged from 1.7×104 to 1.6×106 colony forming units (CFU)/m3 (TSP); from 3.6×103 to 3.0×104 CFU/m3 (PM10); and from 3.1×103 to 1.6×104 CFU/m3 (PM2.5). Our results will improve the knowledge on essential particle characteristics necessary to understand PM’s origin in rabbit farms and contribute to its reduction.


Keywords

Air quality; animal housing; bioaerosol; dust; SEM-EDX

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References

Aarnink A.J.A., Stockhofe-Zurwieden N., Wagemans M.J.M., 2004. Dust in different housing systems for growing-finishing pigs. Proceedings of Engineering the Future. AgEng 2004. Leuven, Belgium.

Adell E., Estellés F., Torres A.G., Cambra-López M., 2012. Particulate matter concentrations and emissions in rabbit farms. World Rabbit Science, 20: 1-11. https://doi.org/10.4995/wrs.2012.1035

Adell E., Moset V., Zhao Y., Cerisuelo A., Cambra-López M., 2011. Concentración, distribución espacial y por tamaño de bacterias aerobias mesófilas en el aire de granjas de broilers. Información Técnica Económica Agraria, 107(2): 77-93.

Al Homidan A., Robertson J.F., 2003. Effect of litter type and stocking density on ammonia, dust concentrations and broiler performance. British Poultry Science, 44: S7-S8.

Andersen C.I., Von Essen S.G., Smith L.M., Spencer J., Jolie R., Donham K.J., 2004. Respiratory symptoms and airway obstruction in swine veterinarians: A persistent problem. American Journal of Industrial Medicine, 46: 386-392. https://doi.org/10.1002/ajim.20080

Bakutis B., Monstviliene E., Januskeviciene G., 2004. Analyses of airborne contamination with bacteria, endotoxins and dust in livestock barns and poultry houses. Acta Veterinaria Brno, 73: 283-289. https://doi.org/10.2754/avb200473020283

Cambra-López M., Aarnink A.J.A., Zhao Y., Calvet S., Torres A.G., 2010. Airborne particulate matter from livestock production systems: A review of an air pollution problem. Environmental Pollution 58: 1-17. https://doi.org/10.1016/j.envpol.2009.07.011

Cambra-López M., Hermosilla T., Lai H.T.L., Aarnink A.J.A., Ogink N.W.M., 2011a. Particulate matter emitted from poultry and pig houses: Source Identification and Quantification. Transactions of the ASABE, 54: 629-642. https://doi.org/10.13031/2013.36466

Cambra-López M., Torres A.G., Aarnink A.J.A., Ogink N.W.M., 2011b. Source analysis of fine and coarse particulate matter from livestock houses. Atmospheric Environment, 45: 694-707. https://doi.org/10.1016/j.atmosenv.2010.10.018

Donham K.J., 1991. Association of environmental air contaminants with disease and productivity in swine. American Journal of Veterinary Research, 52: 1723-1730.

Donham K.J., Leininger J.R., 1984. Animal studies of potential chronic lung-disease of workers in swine confinement buildings. American Journal of Veterinary Research, 45: 926-931.

Donham K.J., Scallon L.J., Popendorf W., Treuhaft M.W., Roberts R.C., 1986. Characterization of dusts collected from swine confinement buildings. American Industrial Hygiene Association Journal, 47: 404-410. https://doi.org/10.1080/15298668691389955

Donham K.J., Zavala D.C., Merchant J.A., 1984. Respiratory symptoms and lung function among workers in swine confinement buildings: a cross-sectional epidemiological study. Archives of Environmental Health, 39: 96-101. https://doi.org/10.1080/00039896.1984.10545842

Duan H.Y., Chai T.J., Muller W., Zucker B.A., 2006. Concentration of airborne endotoxins and airborne bacteria in Chinese rabbit houses. Berliner und Munchener Tierarztliche Wochenschrift, 119: 40-44. PMid:16450707

Feddes J.J.R., Cook H., Zuidhof M.J., 1992. Characterization of airborne dust particles in turkey housing. Canadian Agricultural Engineering, 34: 273-280.

Grantz D.A., Garner J.H.B., Johnson D.W., 2003. Ecological effects of particulate matter. Environment International, 29: 213-239.

http://dx.doi.org/10.1016/S0160-4120(02)00181-2

Harrison R.M., Yin J.X., 2000. Particulate matter in the atmosphere: which particle properties are important for its effects on health? Science of the Total Environment, 249: 85-101. https://doi.org/10.1016/S0048-9697(99)00513-6

Heber A.J., Stroik M., Faubion J.M., Willard L.H., 1988. Size distribution and identification of aerial dust particles in swine finishing buildings. Transactions of the ASAE, 31: 882-887. https://doi.org/10.13031/2013.30794

McCrone, 2007. McCrone Atlas of microscopic particles. Available at: http://www.mccroneatlas.com. Accesed January 2011.

Navarotto P., Crimella C., Luzi F., Guarino M., Heinzl E., Dragoni I., Papa A., Massola A., 1995. Air-quality in the environment of intensively reared fattening rabbits. Farm building progress, 28-32.

Ribikauskas V., Ribikauskiene D., Skurdeniene I., 2010. Effect of housing system (wire cage versus group-housing) and in-house air quality parameters on the behaviour of fattening rabbits. World Rabbit Science 18, 243-250. https://doi.org/10.4995/wrs.2010.777

SAS. (2001). SAS User's Guide: Statistics. SAS Institute Inc., Cary NC, USA.

Schneider F., Engelhardt T., Wieser P., 2001. Characterizarion of aerosol particles from animal husbandry with single particle analytic techniques. ASAE Meeting presentation, 1-10.

Seedorf J., Hartung J., Schroder M., Linkert K.H., Phillips V.R., Holden M.R., Sneath R.W., Short J.L., White R.P., Pedersen S., Takai H., Johnsen J.O., Metz J.H.M., Koerkamp P.W.G.G., Uenk G.H., Wathes C.M., 1998. Concentrations and emissions of airborne endotoxins and microorganisms in livestock buildings in Northern Europe. Journal of Agricultural Engineering Research, 70: 97-109. http://dx.doi.org/10.1006/jaer.1997.0281

Sirotek K., Santos E., Benda V., Marounek M., 2003. Isolation, identification and characterization of rabbit caecal mucinolytic bacteria. Acta Veterinaria Brno 72: 365-370. https://doi.org/10.2754/avb200372030365

Thorne P.S., Kiekhaefer M.S., Whitten P., Donham K.J., 1992. Comparison of bioaerosol sampling methods in barns housing swine. Applied and Environmental Microbiology, 58: 2543-2551. https://doi.org/10.1128/AEM.58.8.2543-2551.1992

Watson J.G., Zhu T., Chow J.C., Engelbrecht J., Fujita E.M., Wilson W.E., 2002. Receptor modeling application framework for particle source apportionment. Chemosphere, 49: 1093-1136. https://doi.org/10.1016/S0045-6535(02)00243-6

Zhang Y., 2004. Indoor air quality engineering. CRC Press, Boca Raton, Florida, 618 pp. https://doi.org/10.1201/b12485

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 Universitat Politècnica de València

 

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