Characteristics of Staphylococcus aureus isolated from acute, sub-acute and sub-clinical staphylococcosis in rabbits

Rajeshkumar J. Tirpude, H.V. Batra

Abstract

Staphylococcus aureus bacteria isolated from different clinical presentations of staphylococcosis in rabbits were examined for the production of various virulence factors using biochemical and immunological tests. In the total of 106 S. aureus isolates; toxic shock syndrome toxin-1, staphylococcal enterotoxin-C, DNase, α-haemolysin, β-haemolysin, δ-haemolysin, protein A and clumping factor were observed with a frequency of 33.2, 16.98, 83.96, 69.81, 36.79, 100, 78.30 and 54.72 percent, respectively. No SE-A, SE-B and SE-D producing isolates were recovered in this study. All the S. aureus isolates from acute staphylococcosis produced TSST-1, SE-C and protein A. While δ–haemolysin and clumping factor were not detected in any acute isolates, these factors were observed at a relatively higher frequency in isolates from sub-acute and sub-clinical staphylococcosis. Coagulase type III was observed more predominantly with a frequency of 45.28%, while coagulase types V and VII were not observed in any isolate. Most of the virulence factors belonged to coagulase type III followed by type VI. TSST-1 and SE-C along with coagulase types III and VI could be correlated with the acute and sub-acute staphylococcal infections in rabbits in this study.


Keywords

Staphylococcus aureus; coagulase typing; virulence factors; rabbits; toxic shock syndrome toxin-1; enterotoxins

Full Text:

PDF

References

Anderson M.R., Tary-Lehmann M. 2001. Staphylococcal enterotoxin-B-induced lethal shock in mice is T-cell-dependent, but disease susceptibility is defined by the non-T-cell compartment. Clin. Immunol., 98: 85-94. http://dx.doi.org/10.1006/clim.2000.4960

Corpa J.M., Hermans K., Haesebrouck F. 2009. Main pathologies associated with Staphylococcus aureus infections in rabbits: a review. World Rabbit Sci., 17: 115-125. http://dx.doi.org/10.4995/ wrs.2009.651

Easmon C.S.F., Goodfellow M. 1990. Staphylococcus and Micrococcus. 162-186. In: Topley and Wilson’s Principles of Bacteriology, Virology and Immunity. 8th ed. (Parker, M.T. and Duerden, B. I. eds.), Edward Arnold, London.

Hermans K., Devriese L.A., Haesebrouck F., 2003. Rabbit staphylococcosis: difficult solutions for serious problems. Vet. Microbiol., 91: 57-64. http://dx.doi.org/10.1016/S0378-1135(02)00260-2

Llewelyn M., Cohen J. 2002. Superantigens: microbial agents that corrupt immunity. Lancet Infect. Dis., 2: 156–162.

http://dx.doi.org/10.1016/S1473-3099(02)00222-0

Matsuda K., Harada B., Shibata K., Sanada M., Nagakawa S. 1992. Bacteriological study on methicillin-resistant Staphylococcus aureus isolated from patients before combination therapy with imipenem/cilastatin sodium and cefotiam. Chemotherapy, 40: 789-798.

Matsunaga T., Kamata S., Kakiichi N., Uchida K. 1993. Characteristics of Staphylococcus aureus isolated from peracute, acute and chronic bovine mastitis. J. Vet. Med. Sci., 55: 297-300. http://dx.doi.org/10.1292/jvms.55.297

Meulemans G., Haesebrouck F., Lipinska U., Duchateau L., Hermans K. 2011. Efficacy of an autogenous vaccine against highly virulent Staphylococcus aureus infection in rabbits. World Rabbit Sci., 19: 1-9. http://dx.doi.org/10.4995/wrs.2011.812

Novick R.P. 2000. Pathogenicity factors and their regulation. In: Gram-positive pathogens. V. A. Fischetti, R. P. Novick, J. J. Ferretti, D. A. Portnoy and J. I. Rood eds., (ASM Press, Washington, D.C), 392-407.

Riffon R., Sayasith K., Khalil H., Dubreuil P., Drolet M., Lagacé J. 2001. Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. J. Clin. Microbiol., 39: 2584-2589. http://dx.doi.org/10.1128/JCM.39.7.2584-2589.2001

Rodríguez-Calleja J.M., García-López I., García-López M.L., Santos J.A., Otero A. 2006. Rabbit meat as a source of bacterial foodborne pathogens. J. Food Protect., 69: 11061112.

Tenover F.C., Gaynes R. P. 2000. The epidemiology of Staphylococcus aureus infections. In: Fischetti V A, Novick R P, Ferretti J J, Portnoy DA, Rood J I, editors. Gram-positive pathogens. Washington, DC, USA: ASM Press. 414-421.

Tirpude R.J., Jain R., Tuteja U., Kumar P., Batra H.V. 2007.Isolation, identification and characterization of Staphylococcus aureus from systemic infection in New Zealand White rabbits. Indian J. Anim. Sci., 77: 207-210.

Vancraeynest D., Hermans K., Haesebrouck F. 2004. Genotypic and phenotypic screening of high and low virulence Staphylococcus aureus isolates from rabbits for biofilm formation and MSCRAMMs. Vet. Microbiol., 103 : 241-247. http://dx.doi.org/10.1016/j.vetmic.2004.09.002

Vancraeynest D., Hermans K., Haesebrouck F. 2006. Prevalence of genes encoding exfoliative toxins, leucotoxins and superantigens among high and low virulence rabbit Staphylococcus aureus strains. Vety. Microbiol., 117 : 211-218.

http://dx.doi.org/10.1016/j.vetmic.2006.05.009

Winblad S., Ericson C. 1973. Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Acta Pathol. Microbiol. Scand., B 81: 150-156. http://dx.doi.org/10.1111/j.1699-0463.1973. tb02199.x

Abstract Views

2927
Metrics Loading ...

Metrics powered by PLOS ALM


 

Cited-By (articles included in Crossref)

This journal is a Crossref Cited-by Linking member. This list shows the references that citing the article automatically, if there are. For more information about the system please visit Crossref site

1. The importance of subcutaneous abscess infection by Pasteurella spp. and Staphylococcus aureus as a cause of meat condemnation in slaughtered commercial rabbits
A. Ferreira, J.M. Monteiro, M. Vieira-Pinto
World Rabbit Science  vol: 22  issue: 4  first page: 311  year: 2014  
doi: 10.4995/wrs.2014.2238



 

 Universitat Politècnica de València

 

Official journal of the World Rabbit Science Association (WRSA)

 

e-ISSN: 1989-8886     ISSN: 1257-5011   https://doi.org/10.4995/wrs