Effects of transgenic poplar leaves with binary insect-resistance genes used as feed for rabbits
DOI:
https://doi.org/10.4995/wrs.2013.1188Keywords:
growing performance, blood haematology and biochemistry, rabbit, transgenic poplarAbstract
The aim of this work was to explore the potential toxicological effects on rabbits of transgenic poplar (Populus cathayana Rehd.) leaves with binary insect-resistance genes used as feed. Fifty-four 40-d-old weaned New Zealand White Rabbits (Oryctolagus cuniculus) (live weight 0.98±0.1 kg) were fed for 70 consecutive days with a common pelleted diet and fresh poplar leaves containing a chitinase-BmkIT gene combination or untransformed counterparts (60 g/d). Rabbit body weight and hematological and biochemical data in blood samples were recorded. Organ histological structures were observed and the organ weights in the 2 groups were also measured. The results of the growth study revealed no significant differences (P>0.05) for final mean BW of rabbits, intake of the combined feed and poplar leaves or feed conversion ratio between the 2 groups. No obvious pathological changes were observed in the small intestine, stomach, spleen, kidney, lung, heart, bladder, pancreas, prostate and ovary. Electron microscopic observation of liver cells and renal cells showed they were both normal in the 2 groups. All hematological and biochemical data tested fell within the normal range in the 2 groups after 70 d of feeding. We conclude that the poplar leaves with the chitinase-BmkIT gene combination had no obvious harmful effects on rabbits.Downloads
References
Akinmutimi A.H. 2004. Evaluation of sword bean (Canavalia gladiata) as an alternative feed resources for broiler chickens. Ph.D Thesis, Umudik, Nigeria, .
Aldrin J.F., Messager J.L., Laurencin F.B. 1982. La biochimie clinique en aquaculture. Intérêt et perspective. CNEXO Actes Colloq., 14: 291-326.
Barton K.A. 1990. Insecticidal toxins in plants-express an insectspecific from a scorpion. European Patent [Patent No.EP0431829A 1].
Betz F.S., Hammond B.G., Fuchs R.L. 2000. Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests. Regul. Toxicol. Pharm., 32: 156-173. doi:10.1006/rtph.2000.1426
Bone F.J. 1979. Anatomy and physiology of farm animals. 2nd Ed, Reston publishing comp. Inc Virginia, USA, pp 560.
Fujimoto H., Itoh K., Yamamoto M., Kyozuka J., Shimamoto K. 1993. Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis. Nat. Biotechnol., 11: 1151-1155. doi:10.1038/nbt1093-1151
Grønsberg I.M., Nordgård L., Fenton K., Hegge B., Nielsen K.M., Bardocz S., Pusztai A., Traavik T. 2011. Uptake and organ distribution of feed introduced plasmid DNA in growing or pregnant rats. Food Nutr. Sci., 2: 377-386. doi:10.4236/fns.2011.24053
Guertler P., Paul V., Steinke K., Wiedemann S., Preißinger W., Albrecht C., Spiekers H., Schwarz F.J., Meyer H.H.D. 2010. Long-term feeding of genetically modified corn (MON810) - Fate of cry1Ab DNA and recombinant protein during the metabolism of the dairy cow. Livest. Sci., 131: 250-259. doi:10.1016/j.livsci.2010.04.010
Kececi T., Oguz H., Kurtoglu V., Demet O. 1998. Effects of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Brit. Poultry Sci., 39: 452-458. doi:10.1080/00071669889051
Kobayashi W. 1985. Electron microscopic observation of the breakdown of cortical vesicles in the chum salmon egg. J. Fac. Sci. Hokaido Univ., 24: 87-102.
Kramer K.J., Corpuz L., Choi H.K., Muthukrishnan S. 1993. Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta. Insect Biochem. Molec., 23: 691-701. doi:10.1016/0965-1748(93)90043-R
Gu H.Y., Lin T., Chen X.J. 2012. Design the application of methane in planting poplars. Forest Engineering, 5: 23-25 (in Chinese with English Abstract).
Marco I., Cuenca R., Pastor J., Velarde R., Lavin S. 2003. Hematology and serum chemistry values of the European brown hare. Vet. Clin. Path., 32: 195-198. doi:10.1111/j.1939-165X.2003.tb00335.x
McNaughton J., Roberts M., Rice D., Smith B., Hinds M., Delaney B., Liams C., Sauber T. 2011. Nutritional equivalency evaluation of transgenic maize grain from event DP-Ø9814Ø-6 and transgenic soybeans containing event DP-356Ø43-5: Laying hen performance and egg quality measures. Poult. Sci., 90: 377-389. doi:10.3382/ps.2010-00973
Marvier M., McCreedy C., Regetz J., Kareiva P. 2007. A metaanalysis of effects of Bt cotton and maize on nontarget invertebrates. Science, 316: 1475-1477. doi:10.1126/science.1139208
Oyawoye E.O., Ogunkunle M. 1998. Physiological and biochemical effects of raw jack beans on broilers. In Proc.: Annual Conference of Nigerian Society of Animal Production, Abeokuta, Nigeria, 23: 141-142.
Qiao G., Park S.I., Xu D.H. 2012. Clinical, hematological, and biochemical alterations in olive flounder Paralichthys olivaceus following experimental infection by Vibrio scophthalmi. Fish. Aquat. Sci., 15: 233-239. doi:10.5657/FAS.2012.0233
Tudisco R., Calabrò S., Bovera F., Cutrignelli M.I., Nizza A., Piccolo V., Infascelli F. 2010. Detection of plant species-specific DNA (barley and soybean) in blood, muscle tissue, organs and gastrointestinal contents of rabbit. World Rabbit Sci., 18: 83-90. doi:10.4995/WRS.2010.18.11
Wang J.X., Chen Z.L., Du J.Z., Sun Y., Liang A.H. 2005. Novel insect resistance in Brassica napus developed by transformation of chitinase and scorpion toxin genes. Plant Cell Rep., 24: 549-555. doi:10.1007/s00299-005-0967-3
Yang L.Y., Sun Y., Xie L.Q. 2008. Bioassays of resistance of transgenic poplar with novel binary insect-resistant genes to Anoplophora glabripennis (Coleoptera: Cerambycidae) and Hyphantria cunea (Lepidoptera: Arctiidea). Acta Entomol. Sinica, 51: 844-848.
Zhang Z.Y., Zhang H.S., Sun Y., Liang A.H. 2004. Construction of plant expression vectors containing two anti-insect genes. Acta Botanica Boreali-occidentalia Sinica, 24: 1402-1408 (in Chinese with English abstract).
Zhou Z.B. 1995. Manual for Medicine Technology (1st ed). Beijing Science and Technology Literature Press. Beijing, P.R. China, 941-942.
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