Fertility potential of rabbit bucks fed maize-based diets containing graded levels of fumonisin B1
DOI:
https://doi.org/10.4995/wrs.2012.1079Keywords:
FB1 tolerance level, feeding trial, reproductive toxicant, spermatozoa production, testicular elements.Abstract
Fumonisin B1-contaminated maize-based diets have been reported to be mycotoxic in animal species, yet more validated data and biomarkers are needed. In this study, Fumonisin B1 (FB1) infected yellow-maize was used to formulate Diets 1, 2 and 3, containing 1,700, 1,800 and 1,900µg FB1/kg diet, respectively. Sixty sexually matured bucks and does were used, but only the bucks were fed the FB1-contaminated diets for eight weeks. At the end of the feeding trial, the treated bucks were mated to the dry does that were fed FB1-free yellow maize-based diet. Effects on testis and live weight, feed utilisation, conception rate, embryo development and spermatozoa production per gram testis were monitored. Results indicated significant depression (P<0.05) in feed intake, from 546.77±12.09g/week in Diet 1 with the lowest FB1 concentration, to 509.84±21.98g/week in Diet 3 with the highest FB1 concentration in the diets. Weight gain was drastically reduced (P<0.05) from 34.13±9.32g/week in Diet 1 to 20.38±22.13g/week in Diet 3. Meanwhile, some of the untreated does were pregnant in all the treatments, indicating that FB1 concentration at 2.0mg/kg diet may not be spermaticidal and there were no abnormalities in the embryos. It was observed that the paired testis weight value in Diet 1 (3.06±0.31) was not significantly different (P>0.05) from that of Diet 3 (2.94±0.23). The testicular elements were distorted by the dietary FB1, but did not follow a definite pattern. Spermatozoa production per gram testis values (31.1 – 37.3 x 106) recorded were much higher than 24 x 106 for a healthy rabbit buck. Consequently, FB1 concentration < 2.0mg/kg diet may not affect the fertility potentials of bucks orally dosed for a relatively short period. This observation further elucidates earlier discoveries that FB1 is not a reproductive toxicant. Hence, the United States Food and Drug Administration proposed a guideline of 2.0mg total fumonisins/kg diet, to be adopted with strict adherence to safeguard healthy living.
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References
AOAC, 2006. Association of Official Analytical Chemists. Official Method 945.38 Washington D.C. Journal of AOAC International, 86: 888(2003), 899(2003).
Bondy G.S., Suzuki C.A.M., Mueller R.W., Fernie S.M., Armstrong C.L., Hierlihy S.L., Savard M.E., Barker M.G, 1998: Gavage administration of the fungal toxin Fumonisin B1 to female Sprague Dawley rats. Journal of Toxicology and Environmental Health, 53: 135 - 151. https://doi.org/10.1080/009841098159411
Bucci T.J., Hansen D.K, LaBorde J.B, 1996: Leukoencephalomalacia and haemorrhage in the brain of rabbits gavaged with mycotoxin fumonisin B1. Natural Toxins, 4: 51 - 52. https://doi.org/10.1002/19960401NT7
Bullerman L.B., Tsai, W - Y, 1994: Incidence and levels of Fusarium moniliforme, F. Proliferatum and fumonisins in corn and corn-based foods and feeds. Journal of food Protection 57: 541 - 546.
Clermont Y, 1972: Kinetics of spermatogonia in mammals: Seminiferous epithelium cycle and spermatogonial renewal. Physiology Rev, 52: 198 - 236. https://doi.org/10.1152/physrev.1972.52.1.198 PMid:4621362
Collins T.F., Shacklelford M.E. Sprando R.L., Black T.N., LaBorde J.B., Hansen D.K., Eppley R.M., Trucksess M.W., Howard P.C., Bryant M.A., Ruggles D.I., Olejnik N, Rorie J.I, 1998: Effects of fumonisin B1 in pregnant rats. Food Chemistry and Toxicology, 36: 367 - 408. https://doi.org/10.1016/S0278-6915(97)00170-1
Courot M, Hocherau-de Reviers M-T, Ortavant R, 1970: Spermatogenesis. In: A.D., Johnson W.R., Gomes, N.L., Vandemark (editors). The Testis. New York Academic Press, USA, pp. 432. https://doi.org/10.1016/B978-0-12-386601-1.50012-3
Culling C.F.A, 1974: Handbook of histopathological and histochemical techniques (3rd edition). Butter worths.
Egbunike G.N, 1979: The effects of micro-doses of Aflatoxin B1 on sperm production rates, epididymal sperm abnormality and fertility in the rat. ZBL. Veterinary Medicine 26: 66 - 72. https://doi.org/10.1111/j.1439-0442.1979.tb00650.x
FDA, 2001: Fumonisin levels in human foods and animal feeds. U.S. Food and Drug Administration, Centre for Food Safety and Applied Nutrition, Centre for Veterinary Medicine.
Floss J.L., Casteel S.W., Johnson G.C., Rottinghus G.E., Krause G.F, 1994: Developmental toxicity of fumonisin in Syrian hamsters. Mycopathologia, 128: 33 - 38. https://doi.org/10.1007/BF01104276
Gelderblom W.C.A., Cawood M.E. Snyman S.O., Marasas W.F.O, 1994: Fumonisin B1 dosimetry in relation to cancer initiation in rat liver. Carcinogenensis, 15: 209 - 214. https://doi.org/10.1093/carcin/15.2.209
Gelderblom W.C.A., Marasas W.F.O., Vleggaar R, Thiel P.G., Cawood M.E, 1992: Fumonisins: Isolation, chemical characterization and biological effects. Mycopathologia, 117: 11 - 16. https://doi.org/10.1007/BF00497273
Guerzoni E.M, 2008: Mycotoxins traceability and risk assessment, prevention or reduction of their biosynthesis. A paper presented at the 3rd Annual Conference/workshop organized by Nigeria Mycotoxin Awareness and Study Network in Lagos, Nigeria. April 28-30, 2008. pp. 1-3.
Gumprecht L.A., Maracucci A., Weigel R.M., Vesonder R.F., Riley R.T., Showker J.L., Beasley V.R., Haschek W.M, 1995: Effects of intravenous fumonisin B1 in rabbit: Nephrotoxicity and sphingolipid alterations. Natural Toxins, 3: 395 - 403. https://doi.org/10.1002/nt.2620030512
Hafez E.S.E, 1970: Reproduction and breeding techniques for laboratory animals. Lea and Febiger, Philadelphia, pp. 334.
Harrison L.R. Colvin B.M., Greene J.T., Newman L.E., Cole J.R. Jr, 1990: Pulmonary oedema and hydrothorax in swine produced by fumonisin B1¬¬, a toxic metabolite of Fusarium moniliforme. Journal of Veterinary and Diagnostic Investigation, 2: 217 - 221. https://doi.org/10.1177/104063879000200312
Hendricks K, 1999: Fumonisins and neural tube defects in South Texas. Epidemiology, 10: 198 - 200.
Lebas F., Coudert P., Rouvier R, de Rochambeau H, 1986: The rabbit: Husbandry, health and production. FAO Animal Production and Health Series 21.
LaBorde J.B., Terry K.K., Howard P.C., Chen J.J., Collin, F.X., Shacklelford M.E, Hansen, D.K, 1997: Lack of embryotoxicity of fumonisin B1 in New Zealand white rabbits. Fundamental and Applied Toxicology, 40: 120 - 128. https://doi.org/10.1006/faat.1997.2380
Lang J, 1981: The nutrition of the commercial rabbit part 1: Physiology, digestibility and nutrient requirements part 2: Feeding and general aspects of nutrition. Nutrition Abstract Rev. Ser. B. Livestock Feeds, 51: 197 - 225; 287 - 302.
Nelson P.E., Ross, P.F, 1992: Fumonisin production by Fusarium species on solid substrates. 106th AOAC Annual Meeting, Cincinnati, OH, August 31 - September 2. Pp. 104
NRC, 1998: Nutrient requirements of Animals. National Research Council (10th edition). National Academy Press, Washington D.C.
Orgebin-Christ M.C, 1968: Gonadal and epididymal sperm reserves in the rabbit: Estimation of the daily sperm production. J. Reprod. and Fert. 15:15 - 25. https://doi.org/10.1530/jrf.0.0150015
Ortavant R, 1959: Spermatogenesis and morphology of the spermatozoa. In: H.H. Cole and P.T. Cupps (editors). Reproduction in Domestic Animals (1st edn). New York, pp. 50 .
Pestka J.J., Azcona-Olivera J.I., Plattner R.D., Minervini F., Doko M.B., and Visconti A, 1994. Comparative assessment of fumonisin in grain-based foods by ELISA, GC-MS and HPLC. Journal of Food Protection., 57: 169 - 172. https://doi.org/10.4315/0362-028X-57.2.169
Reddy R.V., Johnson G., Rottinghaus G.E., Casteel S.W., Reddy, C.S, 1996: Developmental effects of fumonisin B1 in mice. Mycopathologia, 134: 161 - 166. https://doi.org/10.1007/BF00436724
SAS, 1999: Institute Incorporation SAS/STAT. Statistical Analysis Software, Users' guide, version 8 for Windows. Cary, NC, USA.
Sanchis V., Abadias M. Oncins L., Sala N., Vinas I, Canela R, 1995: Fumonisins B1 and B2 and toxigenic Fusarium strains in feeds from the Spanish market. International Journal of Food Microbiology, 27: 37 - 44. https://doi.org/10.1016/0168-1605(94)00151-U
Somade B, 1985: Influence of heterospermic mating on the reproductive performance of rabbits. Journal of Animal Production Resources, 5 (1): 13 - 32.
Swiestra E.E, 1966: Structural composition of shorthorn bull testes and daily spermatozoa production as determined by quantitative testicular histology. Canadian Journal of Animal Science, 46: 107 - 119. https://doi.org/10.4141/cjas66-016
Voss K.A., Bacon C.W., Norred W.P., Chapin R.E., Chamberlain W.J., Plattner R.D, Meredith F.I, 1996: Studies on the reproductive effects of Fusarium moniliforme culture material in rats and the biodistribution of [14C] fumonisin B1 in pregnant rats. Natural Toxins, 4: 24 - 33. https://doi.org/10.1002/19960401NT4
WHO, 2000 Monographs on Environmental Health Criteria (219) for fumonisin B1. World Health Organization Programme on Chemical Safety, Geneva. Pp. 126. Available at: http.//www.inchem.org/documents/ehc/ehc/ehc219.htm. Accessed 22 May 2002.
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