Performance, haemato-biochemical indices and antioxidant status of growing rabbits fed on diets supplemented with Mucuna pruriens leaf meal
The effects of dietary Mucuna pruriens leaf meal (MLM) supplementation on rabbits’ performance, haemato-biochemical indices and antioxidant status outside their thermal neutrality zone (21 to 25°C) were evaluated. One hundred and twenty 35-d old crossbreed (Chinchilla×New Zealand) rabbits weighing 694±5 g were allotted to 4 treatments (30 rabbits/treatment; 3 rabbits/replicate). A basal diet (crude protein: 16.9%, crude fibre: 17.6%, digestible energy: 2671 kcal/kg) was divided into 4 equal portions i.e. diets 1, 2, 3 and 4, supplemented with 0, 4, 8 and 12 g MLM/kg, respectively, and pelleted. The average body weight in rabbits fed on diets 3 and 4 was higher compared to those fed on diet 1 (control) at 91 d of age (+228 and +262 g, respectively; P=0.01). Within 35 to 91 d, the average daily weight gain in rabbits fed on diets 3 and 4 was higher compared to those fed on the control diet (+4.1and +4.8 g/d, respectively; P=0.01). The dressing-out percentage of rabbits fed on diets 3 and 4 increased (P=0.05) compared to those fed the control diet. At 63 d and 91 d of age, the white blood cell level of rabbits fed on diet 4 increased significantly compared to those fed the control diet (+5.05×109 and +5.32×109/L, respectively). At 63 and 91 d of age, the cholesterol level of rabbits fed on diets 3 (–1.0 and –1.16 mmol/L, respectively) and 4 (–1.10 and –1.21 mmol/L, respectively), were significantly lower compared to those fed on the control diet. The aspartate aminotransferase (AST) concentration in rabbits fed on diet 4 was reduced compared to those on control diet at 63 d of age (–33.68 IU/L; P=0.02). At 63 d and 91 d of age, compared to control, the activities of glutathione peroxidase in rabbits fed on diets 3 (+35.77 and +49.09 mg protein, respectively) and 4 (+54.52 and +55.02 mg protein, respectively) increased significantly, while catalase activities in rabbits fed diet 4 (+217.7 and +209.5 mg/g, respectively) also increased significantly. It could be concluded that dietary MLM supplementation enhanced the rabbits’ performance, reduced serum AST and cholesterol and improved the antioxidant status.
Adekonla A.Y., Ayo J.O. 2009. Effect of road transportation on erythrocyte osmotic fragility of pigs administered ascorbic acid during the harmattan season in Zaria, Nigeria. J. Cell Anim. Biol., 3: 4-8.
Aebi H. 1974. Catalase estimation In (ed. HV Bergmeyer). Methods of Enzymatic Analysis. Verlag Chemic, New York Academic Press, New York, US. https://doi.org/10.1016/B978-0-12-091302-2.50032-3
Ansari J., Khan S.H., Hag A., Yousaf M. 2012. Effect of the level of Azadirachta indica dried leaf meal as phytogenic feed additive on growth performance and haemato-biochemical parameters in broiler chicks. J. Appl. Anim. Res., 40: 336-345. https://doi.org/10.1080/09712119.2012.692329
AOAC. 1990. Official methods of analysis of the Association of Official Analytical Chemists, 15th ed. AOAC, Washington, D.C. USA.
Attia Y., Bakhashwaina A.A., Bertu N.K. 2017. Thyme oil (Thyme vulgaris L) as a natural growth promoter for broiler chickens reared under hot climate. It. J. Anim. Sci., 16: 275-282. https://doi.org/10.1080/1828051X.2016.1245594
Avellini L., Silverstrelli M., Gaitti A. 1995. Training-induced modifications in some biochemical defenses against free radicals in equine erythrocytes. Vet. Res. Com., 19: 179-184. https://doi.org/10.1007/BF01839296
Ayodele S.O., Oloruntola O.D., Agbede J.O. 2016. Effect of Alchornea cordifolia leaf meal inclusion and enzyme supplementation on performance and digestibility of rabbits. World Rabbit Sci., 24: 201-2016. https://doi.org/10.4995/wrs.2016.3933
Blasco A., Ouhayoun J., Masoero G. 1993. Harmonization of criteria and terminology in rabbit meat research. World Rabbit Sci., 1: 3-10. https://doi.org/10.4995/wrs.1993.189
Brewer N.R., Cruise L.J. 1994. The Biology of the Laboratory Rabbit. 2nd Edition. In: Patrick J. Manning, Daniel H. Ringler, and Christian E. Newcomer (eds.). Elsevier Inc. pp: 483.
Burnett N., Mathura K., Metivier K.S., Holder R.B., Brown G., Campbell M. 2003. An investigation into haematological and serum chemistry parameters of rabbits in Trinidad. World Rabbit Sci., 14: 175-187. https://doi.org/10.4995/wrs.2006.556
Cardoso V.D.S., Lima C.A.R.D., Lima M.E.F.D., Dorneles L.E.G., Danelli M.D.G.M. 2012. Piperin as a phytogenic additive in broiler diets. Pesq. Agropec. Bras., 47: 489-496. https://doi.org/10.1590/S0100-204X2012000400003
de Blas C., Mateos G.G. 2010. Feed formulation. In: Nutrition of the Rabbit. 2nd Edition (eds. C.de Blas and J. Wiseman). CAB International. The United Kingdom. pp. 229. https://doi.org/10.1079/9781845936693.0222
Devi S.M., Park J.W., Kim I.H. 2015. Effect of plant extract on growth performance and insulin-like growth factor 1 secretion in growing pig. Rev. Bras. Zootec., 44: 355-360. https://doi.org/10.1590/S1806-92902015001000003
Duke A.T. 1995. Handbook of Medicinal Herbs. 3rd ed. CRS Press. London, pp. 220.
Edeoga G.O., Okwu D.E., Mbaebie B.O. 2005. Phytochemical constituents of some Nigerian medicinal plants. Afr. J. Biotechnol., 4: 685-688. https://doi.org/10.5897/AJB2005.000-3127
El-Desoky N.I., Hashem N.M., Elkomy A., Abo-Elezz Z.R. 2017. Physiological response and semen quality of rabbit buck supplemented with Moringa leaves ethanolic extract during summer season. Animal., 14: 1-9. https://doi.org/10.1017/S1751731117000088.
El-Gindy Y.M., Zeweil H.S. 2017. Effects of parsley supplementation on the seminal quality, blood lipid profile and oxidant status of young and old male rabbits. World Rabbit Sci., 25: 215-223. https://doi.org/10.4995/wrs.2017.6532
Ferguson NM. 1956. A textbook of pharmacology. McMillan Company. New Delhi. 191.
Fernández-Carmona J., Blas E., Pascual J.J., Maertens L., Gidenne T., Xiccato G., Garcia J. 2005. Recommendations and guidelines for applied nutrition experiments in rabbits. World Rabbit Sci., 13: 209-228. https://doi.org/10.4995/wrs.2005.516
Gyamfi M.A., Yonamine M., Aaniya Y. 1999. Free radical scavenging action of medicinal herbs from Ghana: Thonningia sanguine on experimentally induced liver injuries. General Pharmacol., 32: 661-667. https://doi.org/10.1016/S0306-3623(98)00238-9
Halliwell B.E., Gutteridge J.M.C. 1989. Lipid Peroxidation: a Radical Chain Reaction, Free Radical in Biology and Medicine, 2nd ed. Oxford University Press, New York, NY, 188-218.
Harborne J.B. 1973. Phytochemical methods, Chapman and Hall Ltd, London, 49-188 pp.
Jiang Z Y., Jiang SQ., Lin YC, Zi PB, Yu DQ, Wu TX. 2007. Effect of soybean isoflavone on growth performance, meat quality and antioxidation in male broiler. Poultry Sci., 86: 1356-1362. https://doi.org/10.1093/ps/86.7.1356
Jimoh A.O., Ewuola E.O., Balogun A.S. 2017. Oxidative stress marker in exotic breeds of rabbits during peak of heat stress in Ibadan, Nigeria. J. Adv. Bio. Biotech., 12: 1-9. https://doi.org/10.9734/JABB/2017/30437
Kamel C. 2000. Natural plant extracts: Clinical remedies bring modern animal production solutions. In: 3rd Conference on sow feed manufacturing in the Mediterranean Region. 31-38.
Konca Y., Kirkpinar F., Mert S., Yurtseven S. 2009. Effect of dietary ascorbic acid supplementation on growth performance, carcass, bone quality and blood parameters in broilers during natural summer temperature. Asian J. Anim.Vet. Advs., 4: 139-147. https://doi.org/10.3923/ajava.2009.139.147
Kone A.P., Cinq-Mars D., Desjardins Y., Guay F., Gosselin A., Saucier L. 2016. Effects of plant extracts and essential oils as feed supplements on quality and microbial traits of rabbit meat. World Rabbit Sci., 24: 107-119. https://doi.org/10.4995/wrs.2016.3665
Kumar A., Rajput G., Dhatwalia V.K., Srivastav G. 2009. Phytocontent screening of Mucuna seeds and exploit in opposition to pathogenic microbes. J. Biol. Environ. Sci., 3: 71-76.
Kumar D.S., Muthu A.K. 2010. Free radical scavenging activity of various extracts of whole plant of Mucuna pruriens (Linn): An in-vitro evaluation. J. Pharm. Res., 3: 718-721.
Kumar S., Kumar A.B.V., Meena K. 2011. Review: Effect of heat stress in tropical livestock and different strategies for its amelioration. J. Stress Physiol. Biochem., 7: 45-54.
Li S., Zhao M., Jiang T., Lv W., Gao S., Zhou Y., Miao Z. 2018. Growth performance and antioxidant status of growing rabbits fed on diets supplemented with Eucommia ulmoides leaves. World Rabbit Sci., 26: 35-41. https://doi.org/10.4995/wrs.2018.7864
Liu H.W., Dong X.F., Tong J.M., Qi Z. 2010. Alfalfa polysaccharides improve the growth performance and antioxidant status of heat-stressed rabbits. Livest. Sci., 131: 88-93. https://doi.org/10.1016/j.livsci.2010.03.004
Lording P.M., Friend S.C.E. 1991. Interpretation of laboratory results. Aust. Vet. Pract., 21: 188-192.
Marai I.F.M., Habeeb A.A.M., Gad A.E. 2002. Rabbit’s productive, reproductive and physiological performance traits as affected by heat stress: A review. Livest. Prod. Sci., 78: 71-90. https://doi.org/10.1016/S0301-6226(02)00091-X
Marco-Jiménez F., García-Diego F.J., Vicente J.S. 2017. Effect of gestational and lactational exposure to heat stress on performance in rabbits. World Rabbit Sci., 25: 17-25. https://doi.org/10.4995/wrs.2017.5728
Masella R., Benedetto R.D., Varì R., Filesi C., Giovannini C. 2005. Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. J. Nutr. Biochem., 16: 577-586. https://doi.org/10.1016/j.jnutbio.2005.05.013
Meineri G., Giacobini M., Forneris G. 2017. Evaluation of physiological parameters of the plasma oxidative status in rabbits. J. Appl. Anim. Res., 45: 315-319. https://doi.org/10.1080/09712119.2016.1190734
Mertens D.R. 2002. Gravimetric determination of amylase-treated neutral detergent fibre in feeds with refluxing in breakers of crucibles: Collaborative study. J. AOAC. Int., 85: 12187-1240.
Misra, H.P., Fridovich, I. 1972. The univalent reduction of oxygen by flavins and quinines. J. Biol. Chem., 247: 188-192.
Muanda F., Kone D., Dicko A., Soulimani R., Younos C. 2011. Phytochemical composition and antioxidant capacity of three Malian medicinal plant parts. J. Evid. Based Complementary Altern. Med., 2011: 21-28. https://doi.org/10.1093/ecam/nep109
Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
Oloruntola O.D., Ayodele S.O., Agbede J.O, Oloruntola D.A, Ogunsipe M.H., Omoniyi I.S. 2016a. Effect of Alchornea cordifolia leaf meal and enzyme supplementation on growth, haematological, immunostimulatory and serum biochemical response of rabbits. Asian J. Biol. Life Sci., 5: 190-195.
Oloruntola O.D., Ayodele S.O., Agbede J.O., Oloruntola D.A. 2016b. Effect of feeding broiler chicken with diets containing Alchornea cordifolia leaf meal and enzyme supplementation. Arch. Zootec., 65: 489-498.
Pulido R., Bravo L., Saura-Calixto F. 2002. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J. Agric Food Chem., 48: 3396-3402. https://doi.org/10.1021/jf9913458
Robertson J.B., Van Soest P.J. 1981. The detergent system of analysis. In: James W.P.T., Theander O. (eds.) The analysis of dietary fibre in food. Marcel Dekker, New York. 123-159.
Rotruck J.T., Pope A.L., Ganther H.E., Hafeman D.G., Hoekstra W.G. 1973. Selenium: Biochemical role as a component of glutathione peroxidase. Science, 179: 588-590. https://doi.org/10.1126/science.179.4073.588
Shad M.A., Nawaz H., Rehma T., Ikram M., 2013. Determination of biochemicals, phytochemicals and antioxidative properties of different part of Cichorium intybus L.: A comparative study. The J. Anim. Plant Sci., 23: 1060-1066.
Sies H. 1997. Oxidative stress: oxidants and antioxidants, Exp. Physiol., 82: 291-295. https://doi.org/10.1113/expphysiol.1997.sp004024
Suresh S., Prithiviraj E., Lakshmi N.V., Ganesh M.K., Ganesh L., Prakash S. 2013. Effect of Mucuna pruriens (Linn.) on mitochondrial dysfunction and DNA damage in epididymal sperm of streptozotocin induced diabetic rat. J. Ethnopharmacol., 145: 32-41. https://doi.org/10.1016/j.jep.2012.10.030
Tawfeek S.S., Hassanin K.M.A., Youssef I.M.I. 2014. The effect of dietary supplementation of some antioxidants on performance, oxidative stress, and blood parameters in broilers under natural summer conditions. J. World’s Poult. Res., 4: 10-19.
Valenzuela-Grijalva N.V., Pinelli-Saavedra A., Muhlia-Almazan A., Domínguez-Díaz D., González-Ríos H. 2017. Dietary inclusion effects of phytochemicals as growth promoters in animal production. J. Anim. Sci. Tech., 58: 8. https://doi.org/10.1186/s40781-017-0133-9
Van-Burden T.P., Robinson W.C., 1981. Formation of complexes between protein and Tannin acid. J. Agric. Food Chem. 1: 77.
Xiccato G., Trocino A. 2010. Energy and protein metabolism and requirements. In: C. de Blass, J. Wiseman (eds.).Nutrition of the rabbit, 2nd edition. CAB International, United Kingdom. pp. 83. https://doi.org/10.1079/9781845936693.0083
Yadav M.K., Upadhyay P., Purohit S., Pandey B.L. Shah H. 2017. Phytochemistry and pharmacological activity of Mucuna pruriens: A review. Int. J. Green Pharm., 11: 69-73. https://doi.org/10.22377/ijgp.v11i02.916
Yilkal T. 2015. Important anti-nutritional substances and inherent toxicants of feeds. Food Sci. Quality Man., 36: 40-47.
Yuan S., Chen D., Zhang K., Yu B. 2007. Effect of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidative enzymes of weaning pigs. Asian-Aust. J. Anim. Sci., 20: 1600-1605.
Zeweil H.S., Elgindy Y.M. 2016. Pomegranate peel as a natural antioxidant enhanced reproductive performance and milk yield of female rabbits. World Rabbit Sci., 24: 207-212. https://doi.org/10.4995/wrs.2016.4025
Metrics powered by PLOS ALM
Cited-By (articles included in Crossref)
This journal is a Crossref Cited-by Linking member. This list shows the references that citing the article automatically, if there are. For more information about the system please visit Crossref site
1. Effect of antioxidants in a liposomal form containing organic iodine of the blood serum biochemical composition and the structure of muscle tissue formation of young rabbits
D Zubochenko, V Pashtetsky, P Ostapchuk, T Kuevda, Ye Zyablitskaya, T Makalish, A Kopylova, V. Breskich, A. Zheltenkov, Y. Dreizis
E3S Web of Conferences vol: 224 first page: 04003 year: 2020
Official journal of the World Rabbit Science Association (WRSA)
e-ISSN: 1989-8886 ISSN: 1257-5011 https://doi.org/10.4995/wrs