The effect of different dietary zinc sources on mineral deposition and antioxidant indices in rabbit tissues


  • Klaudia Čobanová Slovak Academy of Sciences
  • Ľubica Chrastinová National Agricultural and Food Centre
  • Mária Chrenková National Agricultural and Food Centre
  • Mária Polačiková National Agricultural and Food Centre
  • Zuzana Formelová National Agricultural and Food Centre
  • Oksana Ivanišinová Slovak Academy of Sciences
  • Miroslav Ryzner Slovak Academy of Sciences
  • Ľubomíra Grešáková Slovak Academy of Sciences



zinc, rabbit, mineral concentration, antioxidant enzymes, lipid peroxidation


The purpose of this study was to compare the effect of dietary zinc from inorganic and organic sources on the concentration of Zn, Cu, Mn and Fe in plasma, tissues and faeces of rabbits. Simultaneously, the activities of total superoxide dismutase (SOD), specific Cu/Zn SOD, glutathione peroxidase (GPx), lipid peroxidation and total antioxidant capacity (TAC) in liver and kidney were also determined. Ninety-six 49-day-old broiler rabbits were allocated to 4 dietary treatments, each replicated 6 times with 4 animals per replicate. For the subsequent 6 wk, the rabbits were fed an identical basal diet (BD) supplemented with an equivalent dose of Zn (100 mg/kg) from different sources. Group 1 (control) received the unsupplemented BD, while the BD for groups 2, 3 and 4 was supplemented with Zn from Zn sulphate, Zn chelate of glycine hydrate (Zn-Gly) and Zn chelate of protein hydrolysate (Zn-Pro), respectively. The intake of dietary Zn sulphate resulted in an increase in Zn plasma concentration (1.85 vs. 1.48 mg/L; P<0.05) compared to the control group. Feeding the diets enriched with Zn increased the deposition of Zn in the liver (P<0.05), irrespective of the Zn source. The addition of Zn-Pro resulted in significantly higher Cu uptake in liver (P<0.05) than in the control and Zn sulphate group (56.0 vs. 35.0 and 36.7 mg/kg dry matter (DM), respectively). Neither Mn nor Fe concentration in plasma and tissues were affected by dietary Zn supplementation, with the exception of Fe deposition in muscle, which was significantly decreased (P<0.05) in rabbits supplemented with inorganic Zn sulphate compared to control and Zn-Gly group (9.8 vs. 13.3 and 12.2 mg/kg DM, respectively). Intake of organic Zn-Gly significantly increased the activities of total SOD (43.9 vs. 35.9 U/mg protein; P<0.05) and Cu/Zn SOD (31.1 vs. 23.8 U/mg protein; P<0.01) as well as TAC (37.8 vs. 31.2 μmol/g protein; P<0.05) in the kidney when compared to that of the control group. The presented results did not indicate any differences between dietary Zn sources in Zn deposition and measured antioxidant indices in rabbit tissues. Higher dietary Zn intake did not cause any interactions with respect to Mn, Cu and Fe deposition in liver and kidney tissues, but did increase the faecal mineral concentrations. Dietary organic Zn-Gly improved the antioxidant status in rabbit kidney.


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Author Biographies

Klaudia Čobanová, Slovak Academy of Sciences

Centre of Biosciences, Slovak Academy of Sciences, Institute of Animal Physiology

Ľubica Chrastinová, National Agricultural and Food Centre

Research Institute for Animal Production

Mária Chrenková, National Agricultural and Food Centre

Research Institute for Animal Production

Mária Polačiková, National Agricultural and Food Centre

Research Institute for Animal Production

Zuzana Formelová, National Agricultural and Food Centre

Research Institute for Animal Production

Oksana Ivanišinová, Slovak Academy of Sciences

Centre of Biosciences. Institute of Animal Physiology

Miroslav Ryzner, Slovak Academy of Sciences

Centre of Biosciences. Institute of Animal Physiology

Ľubomíra Grešáková, Slovak Academy of Sciences

Centre of Biosciences, Slovak Academy of Sciences. Institute of Animal Physiology


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