Identification and profiling of microRNA between back and belly Skin in Rex rabbits (Oryctolagus cuniculus)


  • Bohao Zhao Yangzhou University
  • Yang Chen Yangzhou University
  • Lin Mu Yangzhou University
  • Shuaishuai Hu Yangzhou University
  • Xinsheng Wu Yangzhou University



Rex rabbit, back skin, belly skin, miRNA, transcriptome


Skin is an important trait for Rex rabbits and skin development is influenced by many processes, including hair follicle cycling, keratinocyte differentiation and formation of coat colour and skin morphogenesis. We identified differentially expressed microRNAs (miRNAs) between the back and belly skin in Rex rabbits. In total, 211 miRNAs (90 upregulated miRNAs and 121 downregulated miRNAs) were identified with a |log2 (fold change)|>1 and P-value<0.05. Using target gene prediction for the miRNAs, differentially expressed predicted target genes were identified and the functional enrichment and signalling pathways of these target genes were processed to reveal their biological functions. A number of differentially expressed miRNAs were found to be involved in regulation of the cell cycle, skin epithelium differentiation, keratinocyte proliferation, hair follicle development and melanogenesis. In addition, target genes regulated by miRNAs play key roles in the activities of the Hedgehog signalling pathway, Wnt signalling pathway, Osteoclast differentiation and MAPK pathway, revealing mechanisms of skin development. Nine candidate miRNAs and 5 predicted target genes were selected for verification of their expression by quantitative reverse transcription polymerase chain reaction. A regulation network of miRNA and their target genes was constructed by analysing the GO enrichment and signalling pathways. Further studies should be carried out to validate the regulatory relationships between candidate miRNAs and their target genes.


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Author Biographies

Bohao Zhao, Yangzhou University

The Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology


Joint International Research Laboratory of Agriculture & Agri-Product Safety

Yang Chen, Yangzhou University

The Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology


Joint International Research Laboratory of Agriculture & Agri-Product Safety

Lin Mu, Yangzhou University

The Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology


Joint International Research Laboratory of Agriculture & Agri-Product Safety

Shuaishuai Hu, Yangzhou University

The Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology


Joint International Research Laboratory of Agriculture & Agri-Product Safety

Xinsheng Wu, Yangzhou University

The Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology


Joint International Research Laboratory of Agriculture & Agri-Product Safety


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