Quercetin directly promotes rabbit ovarian steroidogenesis





nutrition, quercetin, ovary, steroids, rabbits


The bioflavonoid quercetin is a component of food with numerous biological effects, but its function in reproductive processes remains to be investigated. This study aimed to examine the direct action of quercetin on steroid hormone release in rabbit ovaries. We analysed the effect of quercetin (0, 1, 10, and 100 ng/mL) on cultured rabbit ovarian fragments. The release of progesterone (P4), testosterone (T) and estradiol (E2) were analysed by enzyme immunoassay. Quercetin promoted P4, T, and E2 release by rabbit ovarian fragments. These observations indicate that quercetin can directly stimulate rabbit ovarian steroidogenesis – an essential regulator of reproduction and fecundity. The application of dietary quercetin for control of rabbit reproduction is discussed.


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Author Biographies

A.V. Sirotkin, Constantine the Philosopher University

Constantine the Philosopher University


Research Institute of Animal Production Nitra, National Agricultural and Food Centre

S. Alwasel, King Saud University

College of Science

A.H. Harrath, King Saud University

College of Science


Anand D.A.V., Arulmoli R., Parasuraman S. 2016. Overviews of biological importance of quercetin: a bioactive flavonoid. Pharmacogn. Rev., 10: 84-89. https://doi.org/10.4103/0973-7847.194044

Beazley K.E., Nurminskaya M. 2016. Effects of dietary quercetin on female fertility in mice: implication of transglutaminase 2. Reprod. Fertil. Dev., 28: 974-981. https://doi.org/10.1071/RD14155

Boots A.W., Haenen G.R.M.M., Bast A. 2008. Health effect of quercetin: from antioxidant to nutraceutical. Eur. J. Pharmacol., 585: 325-337. https://doi.org/10.1016/j.ejphar.2008.03.008

Chen C., Zhou, J.J., 2010a. Quercetin: A potential drug to reverse multidrug resistance. Life Sci., 87: 333-338. https://doi.org/10.1016/j.lfs.2010.07.004

Chen Z.G., Luo L.L., Xu J.J., Zhuang X.L., Kong X.X., Fu Y.C., 2010b. Effects of plant polyphenols on ovarian follicular reserve in ageing rats. Biochem. Cell. Biol., 88: 737-45. https://doi.org/10.1139/O10-012

Münster E. 1989. Entwicklung von enzymimmunologischen Messverfahren auf Mikrotitrationsplatten zur Bestimmung von Testosteron und Progesteron im Blutplasma. Doctoral Thesis. Institut for Animal Production and Breeding of the University of Hohemheim. 154.

Naseer Z., Ahmad E., Epikmen E.T., Uçan U., Boyacioğlu M., İpek E., Akosy M. 2017. Quercetin supplemented diet improves follicular development, oocyte quality, and reduces ovarian apoptosis in rabbits during summer heat stress. Theriogenology, 96: 136-141. https://doi.org/10.1016/j.theriogenology.2017.03.029

Nna V.U., Usman U.Z., Ofutet E.O., Owu D.U. 2017. Quercetin exerts preventive, ameliorative and prophylactic effects on cadmium chloride - induced oxidative stress in the uterus and ovaries of female Wistar rats. Food Chem. Toxicol., 102: 143-155. https://doi.org/10.1016/j.fct.2017.02.010

Prakash B.S., Meyer H.H., Schallenberger E., van de Wiel D.F. 1987. Development of a sensitive enzyme immunoassay (EIA) for progesterone determination in unextracted bovine plasma using the second antibody

technique. J. Steroid Biochem. Mol. Biol., 28: 623-627. https://doi.org/10.1016/0022-4731(87)90389-X

Rice S., Mason H.D., Whitehead S.A. 2006. Phytoestrogens and their low dose combinations inhibit mRNA expression and activity of aromatase in human granulosa-luteal cells. J. Steroid Biochem. Mol. Biol., 101: 216-225. https://doi.org/10.1016/j.jsbmb.2006.06.021

Santini S.E., Basini G., Bussolati S., Grasselli F. 2009. The phytoestrogen quercetin impairs steroidogenesis and angiogenesis in swine granulosa cells in vitro. J. Biomed. Biotechnol., 2009: 419891. https://doi.org/10.1155/2009/419891

Sharma A., Kashyap D., Sak K., Tuli H.S., Sharma A.K. 2018. Therapeutic charm of quercetin and its derivatives: a review of research and patents. Pharm. Pat. Anal., 7: 15-32. https://doi.org/10.4155/ppa-2017-0030

Shu X., Hu X.J., Zhou S.Y., Xu C.L., Qiu Q.Q., Nie S.P., Xie M.Y. 2011. [Effect of quercetin exposure during the prepubertal period on ovarian development and reproductive endocrinology of mice]. Yao Xue Xue Bao, 46: 1051-1057.

Sirotkin A.V. 2014. Regulators of ovarian functions. New York: Nova Science Publishers Inc. 194, ISBN 978-1-62948-574-4.

Sirotkin A.V., Harrath A.H. 2014. Phytoestrogens and their effects. Eur J Pharmacol., 741: 230-236. https://doi.org/10.1016/j. ejphar.2014.07.057

Sirotkin A.V., Chrenek P., Kolesarová A., Parillo F., Zerani M., Boiti C. 2014. Novel regulators of rabbit reproductive functions. Anim. Reprod. Sci., 148: 188-196. https://doi.org/10.1016/j.anireprosci.2014.06.001

Sirotkin A.V., Kadasi A., Stochmalova A., Balazi A., Földesiová M., Makovicky P., Chrenek P., Harrath A.H. 2017. Effect of turmeric on the viability, ovarian folliculogenesis, fecundity, ovarian hormones and response to luteinizing hormone of rabbits. Animal. 26: 1-8. https://doi.org/10.1017/S175173111700235X

van Duursen M.B.M. 2017. Modulation of estrogen synthesis and metabolism by phytoestrogens in vitro and the implications for women’s health. Toxicol Res (Camb)., 6: 772-794. https://doi.org/10.1039/C7TX00184C

Walgren R.A., Lin J.T., Kinne R.K., Walle T. 2000. Cellular uptake of dietary flavonoid quercetin 4’-beta-glucoside by sodiumdependent glucose transporter SGLT1. J. Pharmacol. Exp. Ther., 294: 837-843.

Whitehead S.A., Lacey M. 2003. Phytoestrogens inhibit aromatase but not 17beta-hydroxysteroid dehydrogenase (HSD) type 1 in human granulosa-luteal cells: evidence for FSH induction of 17beta-HSD. Hum. Reprod.,18: 487-494. https://doi.org/10.1093/humrep/deg125